FastQCFastQC Report
Mon 26 Jun 2023
ERR3949775_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949775_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138367948
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGATGATCCTATGCTGTCGAGAAAAGCC5382788538.90198978740366No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC110613467.994153385869391No Hit
GGATGATCCTATGCTGTCGAGAAAAGCC18991491.372535350455584No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAG10696960.7730807715671262No Hit
TGGATGATCCTATGCTGTCGAGAAAAGC9642220.6968535805705524No Hit
ACCCTGTGGGAGAGTAGGACGCCGCCGA8622130.6231305822357068No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGA7385290.5337428289389679No Hit
AAGTGGATGATCCTATGCTGTCGAGAAAAGCC6901510.49877952949045684No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG6096800.44062227474819526No Hit
GGGACGGACGAACCTCTGGTGTGCCAGT5857670.42334009318400817No Hit
CGGGTGTAGTTTAATGGTAGAACATGAG5280410.3816208938792675No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC4957180.35826071511879326No Hit
TGGGTGATCCTATGCTGTCGAGAAAAGCC4579810.3309877804937889No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGG4344880.31400913743405373No Hit
TGGATGATCCTATGCTGTCGAGAAAGGCC4261350.3079723347490851No Hit
CGGGACGGACGAACCTCTGGTGTGCCAGT4240480.30646403746624906No Hit
GGGGTGTTGGGGACTCACGGGAGACCGCCGGGG4184990.30245371565385937No Hit
AATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA3826530.2765474270096135No Hit
TAGATGATCCTATGCTGTCGAGAAAAGCC3355190.24248317970286007No Hit
AGGATGATCCTATGCTGTCGAGAAAAGCC3300260.238513329691064No Hit
CGGATGATCCTATGCTGTCGAGAAAAGCC3032420.21915624563573063No Hit
AGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATC2844220.20555482979338538No Hit
TAGCGGGGCTCAAGCGTACCGCCGAAGTCGTGTCAATC2745000.19838409398107137No Hit
TGAATGATCCTATGCTGTCGAGAAAAGCC2741990.19816655805288086No Hit
TGGATGATCCTATGCTGTCGAGAAGAGCC2557580.18483904957526726No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCC2474100.17880586044392305No Hit
GAGGCGTTCGGGTGAACTATGGTTAAGGA2374230.1715881484344915No Hit
TCGATGATCCTATGCTGTCGAGAAAAGCC2273760.16432707378156683No Hit
CCCTGTGGGAGAGTAGGACGCCGCCGA2255390.16299945417995212No Hit
TGGATGATCCTATGCTGTCGGGAAAAGCC2117000.1529978604582616No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGC2097620.15159724707343353No Hit
TGGATGGTCCTATGCTGTCGAGAAAAGCC2051130.14823736491344078No Hit
TGGTTGATCCTATGCTGTCGAGAAAAGCC2012080.1454151795327629No Hit
TGCATGATCCTATGCTGTCGAGAAAAGCC2003310.1447813622270383No Hit
CTTGGGCTGCACACGTGCTACAATGGCCGGTACAA1969900.1423667856951958No Hit
TGGATGACCCTATGCTGTCGAGAAAAGCC1939100.14014083666254848No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCC1918600.1386592796765332No Hit
TGGACGATCCTATGCTGTCGAGAAAAGCC1895000.13695368236580338No Hit
ATGTCGGCTCGTCGCATCCTGGGGCT1890620.13663713506830352No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCC1850730.13375424198673525No Hit
TGGATGATCCTATGCCGTCGAGAAAAGCC1848340.13358151412348762No Hit
TGGATGATCCTATGCTGCCGAGAAAAGCC1740430.12578274269124812No Hit
ATGTACACGGAGTCGACCCAACGCG1731740.12515470707132262No Hit
ATACCGAGGCGTTCGGGTGAACTATGGTTAAGGA1714400.12390152667437115No Hit
TGGATGATCCTATGCTGTCGAGGAAAGCC1683450.12166473698085052No Hit
TGGATGATCCTGTGCTGTCGAGAAAAGCC1587280.1147144279396266No Hit
CGGGACGGACGAACCTCTGGTGTGCCAG1572660.11365782485984399No Hit
AGGCGTTCGGGTGAACTATGGTTAAGGAAC1521030.10992646938726013No Hit
GACCCTGTGGGAGAGTAGGACGCCGCCGA1467050.10602527689432814No Hit
TGGATGATCCTACGCTGTCGAGAAAAGCC1456290.10524764015435135No Hit
TAAGTGGATGATCCTATGCTGTCGAGAAAAGCC1399550.101146979501351No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)