FastQCFastQC Report
Thu 29 Jun 2023
ERR3949776_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR3949776_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192788031
Total Bases5.7 Gbp
Sequences flagged as poor quality0
Sequence length15-51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGGTGTAGTTTAATGGTAGAACATGAG1998758010.367645696843079No Hit
CGGGTGTAGTTTAATGGTAGAACATGAG131357526.813572363317514No Hit
GAAGTGGATGATCCTATGCTGTCGAGAAAAGCC116611786.048704340986812No Hit
CGGGACGTGGCGCAGCTTGGTAGCGCACTTGAC53812992.791303470493975No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG52960352.7470766585089503No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCG46705592.422639505042717No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTGG46566362.415417583677692No Hit
TGGATGATCCTATGCTGTCGAGAAAAGCC41378602.1463261897207717No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG35529001.8429048637360688No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCC27296741.4158939151155083No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCC23846781.2369429718383296No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGG20250801.050417906908339No Hit
CGCGGGGTGGAGCAGCTCGGTAGCTCGCTGG19237300.9978472159404959No Hit
CGGGTGTAGTTTAATGGTAGAACATGAGC17391190.9020886779013786No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGC14810610.7682328577752838No Hit
TGGCCGCAGAGACCAGCGAGAAGCGACTGTTTAC14570550.7557808399422887No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCG14473430.7507431828068206No Hit
CGGGGTGGAGCAGCTCGGTAGCTCGCTGGG13170820.6831762289226347No Hit
GCGGGTGTAGTTTAATGGTAGAACATGAGC12517310.6492783776602812No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCAC10034310.5204840750720671No Hit
TCGGGTGTAGTTTAATGGTAGAACATGAG8806860.45681570346034606No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAGCCGG8654720.4489241347145663No Hit
GCCGTGGATGATCCTATGCTGTCGAGAAAAGCC7922950.4109669028156629No Hit
AGGGTGTAGTTTAATGGTAGAACATGAG7845550.4069521307575365No Hit
GGGTGTAGTTTAATGGTAGAACATGAG7680670.3983997326058068No Hit
AGGGTCGTAGCTCAATTGGTAGAGCACTG6911850.3585207009038855No Hit
GAGGGTGTAGTTTAATGGTAGAACATGAG6178290.3204706209173328No Hit
GCCCTCATCGTCTAGTGGCCCAGGACGCCGC5818700.301818529387854No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGGT5514360.28603227966989303No Hit
GCGGACGTGGCTCAGTTGGTAGAGCATCACC5500930.28533565966032404No Hit
CGGGTGTAGTTTAATGGTAGAACAT5474250.2839517563203911No Hit
GCACTCGTAGCTTAACGGATAGAGCATCT5469020.2836804739190474No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCC5171070.268225676312862No Hit
GCACTCGTAGCTTAACGGATAGAGCATCTG5044950.2616837764165971No Hit
GACGTGGATGATCCTATGCTGTCGAGAAAAGCC4348580.22556275809466614No Hit
CCCCTGTAGCTCAATTGGCAGAGCAGCCGG4289580.22250240213304529No Hit
GCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG4143680.21493450493303706No Hit
CGCTGAGAACTTAAGCGCCAGTAAACGGCGGTGG4107500.2130578324128431No Hit
TGGCCTATGGTGTAATTGGCAGCACGACT3947230.20474455698964011No Hit
GCGGGTGTAGTTGAATGGTAGAACATGAG3864220.2004387917629596No Hit
CGGGTCGTAGCTCAATTGGTAGAGCACTGG3833320.1988359951661107No Hit
AGGGTCGTAGCTCAATTGGTAGAGCAC3602190.18684718036256098No Hit
CCTCATCGTCTAGTGGCCCAGGACGCCG3397360.17622255813173382No Hit
CGGGGTGTAGTTTAATGGTAGAACATGAG3377600.17519759823679096No Hit
CCCTGTAGCTCAATTGGCAGAGCAGCCGG3339030.17319695536493132No Hit
CACTCGTAGCTTAACGGATAGAGCATCT3313090.1718514361506187No Hit
GCGGGTGTAGTTTAATGGTAGAACAT3306060.17148678695722558No Hit
CCCTCATCGTCTAGTGGCCCAGGACGCCG3287060.1705012485967036No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCGGTG3229390.16750988032031927No Hit
AGGGACGTGGCGCAGCTTGGTAGCGCACTTGAC3182030.16505329628061816No Hit
AGCGGGGTGGAGCAGCTCGGTAGCTCGCTGGG3132950.16250749508406984No Hit
GAAGTGGATGATCCTATGCTGTCGAGACAAGCC3112610.16145245033391104No Hit
CTGGTGTAGTTTAATGGTAGAACATGAG3071440.15931694431798No Hit
CGGACGTGGCTCAGTTGGTAGAGCATCACC3055540.15849220432154318No Hit
CGGACGTGGCTCAGTTGGTAGAGCATCAC2932450.1521074718585616No Hit
ACGGGTGTAGTTTAATGGTAGAACATGAG2825950.14658326999563576No Hit
TAGGGTGTAGTTTAATGGTAGAACATGAG2817290.14613407198499787No Hit
ATGGTGTAGTTTAATGGTAGAACATGAG2796060.14503286254321462No Hit
AGCTGAGAACTTAAGCGCCAGTAAACGGCG2730740.14164468540062014No Hit
CACTCGTAGCTTAACGGATAGAGCATCTG2693390.13970732446559403No Hit
GGGGCGGTAGCTCAGCCGGTTAGAGCAGCGG2681040.13906672453125474No Hit
GCGGTGTAGTTTAATGGTAGAACATGAG2500750.12971500289870175No Hit
CCGGGTGTAGTTTAATGGTAGAACATGAG2427400.12591030612268664No Hit
GCCCCAATAGCTCAGTCGGTAGAGCGTCTC2425210.12579670985902647No Hit
CGCTGAGAACTTAAGCGCCAGTAAACGGCGG2353080.12205529501984487No Hit
GCAGTGGATGATCCTATGCTGTCGAGAAAAGCC2352440.1220220979382273No Hit
GGGGGTGTAGTTTAATGGTAGAACATGAG2343580.1215625258395839No Hit
GGGTCGTAGCTCAATTGGTAGAGCACTGG2256780.11706017164519929No Hit
GCCCTCATCGTCTAGTGGCCCAGGACG2160370.11205934252215066No Hit
GGGATGATCCTATGCTGTCGAGAAAAGCC2076270.10769703851584024No Hit
TGGGTGTAGTTTAATGGTAGAACATGAG2068550.10729659871882814No Hit
GCGGGTGTAGTTTACTGGTAGAACATGAG2033520.10547957720466578No Hit
TCCCCTGTAGCTCAATTGGCAGAGCAG2027210.10515227472809242No Hit
ACTCGTAGCTTAACGGATAGAGCATCT2017080.10462682717061413No Hit
ACTCGTAGCTTAACGGATAGAGCATCTG1968150.10208880654006991No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)