FastQCFastQC Report
Mon 26 Jun 2023
ERR4164643_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4164643_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44308022
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length35-51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGANNNNNNNN1130002325.503334362341878No Hit
CAAGAGGTGCACAATCGACCGATCCTGANNNNNNN20695714.670872015004416No Hit
CAATATAGATGGATACGAATAAGGCGTCNNNNNNN19032334.295459183440867No Hit
CAATATAGATGGATACGAATAAGGCGTCCNNNNNN10632312.3996354429904363No Hit
AATATAGATGGATACGAATAAGGCGTCNNNNNNNN10484232.3662148583387452No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTNNN10073832.2735905475536686No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8613241.9439459518188378No Hit
AGAGGTGCACAATCGACCGATCCTGANNNNNNNNN7784761.7569640098129409No Hit
AATATAGATGGATACGAATAAGGCGTCCNNNNNNN5220791.1782945309542368No Hit
AAGAGGTGCACAATCGACCGATCCTGNNNNNNNNN5096501.1502431771835808No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCANNNN4918881.1101556282516967No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGNNNNNN4063290.9170551553847291No Hit
CAAGAGGTGCACAATCGACCGATCCTGNNNNNNNN3995170.901680964228103No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCANNN3959270.8935785939620595No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTNNNN3754630.847392826517961No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCNNNNN3552220.8017103539399705No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCNNNN3113290.7026470285674228No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTNNN3009220.6791591825064996No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTNNNNN2596000.5858984181239235No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTNN2566090.5791479475206543No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCANNNNNNN2534790.5720837639739369No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATNN1908340.4306985312953036No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCNNNNNNNN1854150.4184682403561143No Hit
TGAATGGCTTAGTGAGGCCTCAGGATCTGCTNNNN1771660.39985084416542No Hit
ATATAGATGGATACGAATAAGGCGTCNNNNNNNNN1629260.3677121944193311No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAANNN1586980.35816990431213563No Hit
GTAATGATTAATAGGGACGGTCGGGGGCATNNNNN1547320.34921892924942577No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTC1531670.345686837476067No Hit
TTCCACGTTCTAGCATTCAAGGTCCCANNNNNNNN1514230.3417507556532314No Hit
TCCGATATAGTGTAACGGCTATCACATCACGNNNN1364350.30792392402441254No Hit
AAGAGGTGCACAATCGACCGATCCTGATNNNNNNN1319160.2977248679708609No Hit
ATATAGATGGATACGAATAAGGCGTCCNNNNNNNN1216770.27461618575525665No Hit
AACTATGCCGACTAGGGATCGGGTGGTGNNNNNNN1187270.26795824918566663No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTNNNN1137900.25681579737411886No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTNNNNNN1062130.23971505656470066No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCNNNNN1058180.23882357014267078No Hit
AATGATTAATAGGGACGGTCGGGGGCATNNNNNNN1034370.23344982540633388No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTNNNNNN994920.22454624582428886No Hit
CCACACAATATAGATGGATACGAATAAGGCGTCNN966850.21821104990875015No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTNNNNNNN935520.21114009557908048No Hit
ATGATTAATAGGGACGGTCGGGGGCATNNNNNNNN917680.2071137366502165No Hit
ATAGATGGATACGAATAAGGCGTCNNNNNNNNNNN910450.20548197795875428No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCNN899300.2029655036282143No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGNNNN895360.2020762741338352No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTNNNNNNN859850.19406192404616934No Hit
ACAATATAGATGGATACGAATAAGGCGTCNNNNNN841400.18989789253061218No Hit
GAATGGCTTAGTGAGGCCTCAGGATCTGCTNNNNN838420.18922532809070106No Hit
ATCGAATCTTTGAACGCACATTGCGCCCCTNNNNN834910.18843314648530235No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTTNNNNN796800.17983199520845233No Hit
TTGCTCGAATATATTAGCATGGAATAATNNNNNNN780690.17619608476316095No Hit
ATAGATGGATACGAATAAGGCGTCCNNNNNNNNNN769710.173717978202683No Hit
AGCGGAGAATTTGGACAAACTTGGTCATTNNNNNN737400.1664258449632439No Hit
CGGGCGTGCTTGTGGACTGCTTGGTGGGGCNNNNN731610.1651190838534837No Hit
AATGATTAATAGGGACGGTCGGGGGCATCANNNNN719940.16248524928510688No Hit
GCAAGAGGTGCACAATCGACCGATCCTGANNNNNN717370.1619052188788748No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTNNNNNNN714160.16118074510299737No Hit
ATGATTAATAGGGACGGTCGGGGGCATCANNNNNN712460.16079706740237693No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATNNN705600.1592488150339909No Hit
AATGATTAATAGGGACGGTCGGGGGCATCNNNNNN676020.1525728230431952No Hit
ATGATTAATAGGGACGGTCGGGGGCATCNNNNNNN643960.14533711299502378No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTNNNNN639580.14434857868401346No Hit
CGTAATGATTAATAGGGACGGTCGGGGGCATNNNN638810.14417479525490892No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCAANNN628850.14192689531480326No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCNNNNNNN623900.14080971612770257No Hit
AAAGAGGTGCACAATCGACCGATCCTGANNNNNNN609970.13766581591026564No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGANN609010.13744915085579762No Hit
AGATGGATACGAATAAGGCGTCCNNNNNNNNNNNN596040.1345219156928287No Hit
TGCTCCACACAATATAGATGGATACGAATAAGGCGTC592880.13380872655520484No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCNNNNNN590060.13317227295770506No Hit
TTGAATGGCTTAGTGAGGCCTCAGGATCTGCTNNN584180.13184519949908843No Hit
TGAAAAAATTAGAGTGTTCAAAGCAGGCGTNNNNN566800.1279226592421571No Hit
TGCCAAGGACGTTTTCATTAATCAAGAACGANNNN553880.12500670871744174No Hit
GTTACGATCTGCTGAGATTAAGCCTTTGTTGTCTGATTTGT549230.12395723735986229No Hit
TTGCTCCACACAATATAGATGGATACGAATAAGGCGTCC545170.12304092473367463No Hit
TTGCTCGAATATATTAGCATGGAATAATAGANNNN537420.12129180580437557No Hit
ACACAATATAGATGGATACGAATAAGGCGTCNNNN532550.12019268203848053No Hit
GTAATGATTAATAGGGACGGTCGGGGGCANNNNNN531040.11985188596322353No Hit
GGGCGTGCTTGTGGACTGCTTGGTGGGGCTNNNNN529370.11947497904555524No Hit
GTGGCGTCGCTGAACCATAGCAGGCTAGCANNNNN527670.11909130134493479No Hit
GGGCGTGCTTGTGGACTGCTTGGTGGGGCNNNNNN519960.11735121012623854No Hit
GTTTCTTTACTTATTCAATGAAGCGGAGNNNNNNN493770.111440316609033No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTNNNNN480730.10849728295250914No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGANNNN479550.10823096548972554No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGNNN475020.10720857726395461No Hit
CCGATATAGTGTAACGGCTATCACATCACGNNNNN466250.10522925171428325No Hit
CAATATAGATGGATACGAATAAGGCGTNNNNNNNN448950.10132476687855757No Hit
GTGGCGTCGCTGAACCATAGCAGGCTAGCNNNNNN447950.10109907411348673No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)