FastQCFastQC Report
Sat 24 Jun 2023
ERR4164644_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4164644_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91809487
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77952258.490653041117636No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGANNNNN7349630.8005305595488187No Hit
AGATGGATACGAATAAGGCGTCCAGANNNNNNNNN4862020.5295770795451672No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGAAGANNNN3923280.4273283870979477No Hit
AAGAGGTGCACAATCGACCGATCCTGAGANNNNNN3212040.3498592689010451No Hit
AGAGGTGCACAATCGACCGATCCTGAAGANNNNNN2863050.31184685739503154No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCAN2706080.29474949576834036No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAAN2393550.2607083514147073No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGAN2245300.24456078269994036No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGAN2156080.23484283274559634No Hit
AGATGGATACGAATAAGGCGTCAGANNNNNNNNNN1970550.2146346814899423No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTAGANNNN1738290.189336642301465No Hit
TGCGAGAGGCCCTGGGTTCAGANNNNNNNNNNNNN1717280.18704820777399617No Hit
CAAGAGGTGCACAATCGACCGATCCTGAAGANNNN1695350.1846595657374711No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAGANNN1592580.17346573344865765No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTAGANN1544950.16827781643088802No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGAN1506920.164135542985879No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCAN1447430.15765582047092802No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGN1432130.1559893260268408No Hit
ATGATGCCCTGACGAGTAGGAGANNNNNNNNNNNN1389460.15134165818833079No Hit
CCGATATAGTGTAACGGCTATCACATCACGAGANN1372970.14954554750970345No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGN1364200.1485903085375044No Hit
ATAGATGGATACGAATAAGGCGTCCAGANNNNNNN1354870.14757407369022768No Hit
ATAGATGGATACGAATAAGGCGTCAGANNNNNNNN1349110.1469466875465713No Hit
AGAGGTGCACAATCGACCGATCCTGAGANNNNNNN1340740.14603501705657063No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCAGANNNNNN1283660.13981779464686475No Hit
AAGAGGTGCACAATCGACCGATAGANNNNNNNNNN1196070.13027738625747903No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGAGN1147830.1250230273043569No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCAGANNNNN1140770.12425404359355585No Hit
ATGTTCCTTGGAACAGGACGTCAGANNNNNNNNNN1100720.11989174931344514No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTTAGANNN1059640.11541726619167363No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTAGANNNNNN1051780.11456114551647587No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGAGANNNNN1043090.11361462024071653No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGN1041570.11344906000836275No Hit
ATGATGCCCTGACGAGTAGGCAGANNNNNNNNNNN1028650.11204179803335575No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTAGANNNNN998840.10879485689752302No Hit
CGGAAGGGCACCACCAGGAGTGGAGCCTGCGGAGN960040.10456871412428217No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)