FastQCFastQC Report
Mon 3 Jul 2023
ERR4164645_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4164645_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82119792
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGANNNNN1238663715.083619549352974No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN73143048.906871074393369No Hit
AGAGGTGCACAATCGACCGATCCTGAAGANNNNNN28265893.4420313680287937No Hit
AAGAGGTGCACAATCGACCGATCCTGAGANNNNNN20552892.5027937235885838No Hit
CAAGAGGTGCACAATCGACCGATCCTGAAGANNNN19304402.3507609468859836No Hit
TTCCACGTTCTAGCATTCAAGGTCCCAAGANNNNN16671542.0301488342785867No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGAN14995051.8259970751021875No Hit
TTCCACGTTCTAGCATTCAAGGTCCCAGANNNNNN13622491.658855882148362No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAGANNNNN13568921.6523324852064896No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAGANNNN13230081.611070812259242No Hit
AAGAGGTGCACAATCGACCGATCCTGATAGANNNN6841940.8331657732401465No Hit
AACTATGCCGACTAGGGATCGGGTGGTGAGANNNN4862600.5921349630305932No Hit
AGAGGTGCACAATCGACCGATCCTGAGANNNNNNN4690530.5711814272495965No Hit
ACTATGCCGACTAGGGATCGGGTGGTGAGANNNNN4531820.5518547830710531No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGNNNNNN4190820.5103300797449657No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAGANNN3718940.4528676838343672No Hit
TTTCCACGTTCTAGCATTCAAGGTCCAGANNNNNN3542570.43139052276216194No Hit
ATGATTAATAGGGACGGTCGGGGGCATCAAGANNN3507010.4270602633771893No Hit
ATGATTAATAGGGACGGTCGGGGGCATAGANNNNN3305120.4024754470883219No Hit
AATGATTAATAGGGACGGTCGGGGGCATCAAGANN3287480.40032736566113075No Hit
ACTATGCCGACTAGGGATCGGGTGGTGTAGANNNN3102860.3778455746697459No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAGAN3032330.3692569021606874No Hit
ATGATTAATAGGGACGGTCGGGGGCATCAGANNNN2912030.35460757133919674No Hit
CAAGAGGTGCACAATCGACCGATCCTGAGANNNNN2907880.35410221204651854No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAGANNN2897280.3528114148170273No Hit
TTCCACGTTCTAGCATTCAAGGTCCAGANNNNNNN2886620.3515133111881238No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAGAN2882560.35101891149456393No Hit
AACTATGCCGACTAGGGATCGGGTGGTGTAGANNN2661970.3241569340555563No Hit
AATATAGATGGATACGAATAAGGCGTCCAGANNNN2549710.31048666075530246No Hit
CAATATAGATGGATACGAATAAGGCGTCCAGANNN2543550.3097365370823151No Hit
AATGATTAATAGGGACGGTCGGGGGCATCAGANNN2499390.30435902711492496No Hit
AATGATTAATAGGGACGGTCGGGGGCATAGANNNN2480040.3020027133044857No Hit
ACGTTCTAGCATTCAAGGTCCCAAGANNNNNNNNN2374050.2890959587428083No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCAGN2303290.28047927836933634No Hit
AATATAGATGGATACGAATAAGGCGTCAGANNNNN2291290.2790179984868934No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGAGAN2029380.24712434731934052No Hit
GAGGTGCACAATCGACCGATCCTGAAGANNNNNNN1946080.23698062946871565No Hit
ATAGATGGATACGAATAAGGCGTCCAGANNNNNNN1771030.215664209183579No Hit
AGATGGATACGAATAAGGCGTCCAGANNNNNNNNN1715140.20885829813110096No Hit
AGAGGTGCACAATCGACCGATCCTGATAGANNNNN1668470.20317513712163324No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGAGAN1668330.20315808885633807No Hit
TCCACGTTCTAGCATTCAAGGTCCCAAGANNNNNN1534450.18685507630121614No Hit
ATTAATAGGGACGGTCGGGGGCATCAAGANNNNNN1517160.18474961553726293No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCAAGN1481260.18037795322228775No Hit
TCCACGTTCTAGCATTCAAGGTCCCAGANNNNNNN1451470.17675032591412312No Hit
ACGTTCTAGCATTCAAGGTCCCAGANNNNNNNNNN1439900.17534140856080102No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTAGANN1428100.17390448334306544No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGCAGANN1421800.17313731140478292No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGN1408210.17148241193791625No Hit
AAGAGGTGCACAATCGACCGATCCTGATGAGANNN1344460.16371936256243805No Hit
TCGAAGAGTCGAGTTGTTTGGGAATGCAGANNNNN1334420.16249675839412744No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGAN1325430.1614020162155306No Hit
AAGAGGTGCACAATCGACCGATCCTGATGTAGANN1313780.15998335699632554No Hit
ATTACCCAATCCTAATTCAGGGAGGTAGTGAGANN1241790.15121689543490321No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGN1232740.15011484685689414No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGAN1232460.1500807503263038No Hit
CCGATATAGTGTAACGGCTATCACATCACGAGANN1176710.1432918875391209No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCTAGANNN1169730.14244190974083323No Hit
CAAGAGGTGCACAATCGACCGATCCTGATAGANNN1105080.13456926437417183No Hit
ATGATTAATAGGGACGGTCGGGGGCAAGANNNNNN1086430.1322981918902084No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGAGTAGN1080580.13158581794751745No Hit
ATAGATGGATACGAATAAGGCGTCAGANNNNNNNN1032390.1257175614862736No Hit
AGGGTGAGAATCCCGTGTGGCGAGGAGTGAGANNN1032270.1257029486874492No Hit
ATATAGATGGATACGAATAAGGCGTCAGANNNNNN1030750.12551785323567308No Hit
ATTAATAGGGACGGTCGGGGGCATCAGANNNNNNN1030590.12549836950390716No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGTAGANN1011250.12314327342670328No Hit
AGAGGTGCACAATCGACCGATCCTGAAGNNNNNNN996040.12129110117570684No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCAGANNNNN979890.11932446200058568No Hit
ACGGTGCACTTGGCGGAAAGGCCTTGGAGANNNNN957010.11653828835806113No Hit
ATGGGGTCTTATGGCTGGAAGAGGCCAGCAAGANN919470.11196691779248538No Hit
ATTAATAGGGACGGTCGGGGGCATAGANNNNNNNN918730.11187680553306809No Hit
TAATGATTAATAGGGACGGTCGGGGGCATCAAGAN866630.10553241537679492No Hit
TGGAAGAGATGTATTTATTAGATAAGANNNNNNNN862250.10499904821970323No Hit
CAATATAGATGGATACGAATAAGGCGTCAGANNNN832420.10136654997859712No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)