FastQCFastQC Report
Fri 23 Jun 2023
ERR4164646_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4164646_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11474890
Total Bases402.6 Mbp
Sequences flagged as poor quality0
Sequence length35-51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGANNNNNNNN255141322.234749091276694No Hit
CAAGAGGTGCACAATCGACCGATCCTGANNNNNNN9169667.991065709562357No Hit
AATATAGATGGATACGAATAAGGCGTCNNNNNNNN2200561.917717729756015No Hit
CAATATAGATGGATACGAATAAGGCGTCCNNNNNN2171521.8924102976150534No Hit
CAAGAGGTGCACAATCGACCGATCCTGNNNNNNNN2151241.874736925582729No Hit
AGAGGTGCACAATCGACCGATCCTGANNNNNNNNN2006161.748304341043792No Hit
AATATAGATGGATACGAATAAGGCGTCCNNNNNNN1993231.7370362591711117No Hit
CAATATAGATGGATACGAATAAGGCGTCNNNNNNN1934581.6859246581012979No Hit
AAGAGGTGCACAATCGACCGATCCTGNNNNNNNNN1590201.3858084914103752No Hit
ATAGATGGATACGAATAAGGCGTCCNNNNNNNNNN1395501.2161336622834729No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCANNNNNNN1369221.1932314819575611No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1321411.151566594538161No Hit
AGATGGATACGAATAAGGCGTCCNNNNNNNNNNNN1271321.107914759967198No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCNNNNNNNN945770.8242083366376498No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTGNNNN827760.7213663921832802No Hit
ATAGATGGATACGAATAAGGCGTCNNNNNNNNNNN787680.6864379527821182No Hit
TCCGATATAGTGTAACGGCTATCACATCACGNNNN710540.6192129074875663No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTNNN630110.5491207323120308No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCANNN627360.5467241951774701No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTAGCNNNN597530.5207283032778527No Hit
AAGAGGTGCACAATCGACCGATCCTGATNNNNNNN587040.5115866034445646No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCNNNNN579440.5049634462726876No Hit
ATATAGATGGATACGAATAAGGCGTCCNNNNNNNN548410.4779217927143528No Hit
TGTGGCGTCGCTGAACCATAGCAGGCTAGCANNNN485290.42291472946581626No Hit
TTCCACGTTCTAGCATTCAAGGTCCCANNNNNNNN420800.3667137549902439No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCNNNNNN412360.3593585646572647No Hit
ATATAGATGGATACGAATAAGGCGTCNNNNNNNNN405690.35354587277089367No Hit
AGATGGATACGAATAAGGCGTCNNNNNNNNNNNNN402460.3507310309728459No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGNNN337000.29368473248981036No Hit
CCGATATAGTGTAACGGCTATCACATCACGNNNNN304240.26513543920682464No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGNNNNNN239730.20891703537027367No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTTNNNNN238810.20811528476525704No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTTNNNN226780.1976315241366148No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCNNN218250.19019790167923178No Hit
GTTCCTTGTCTATGTTCCTTGGAACAGGACGTCNN211890.18465536488802942No Hit
CAAGAGGTGCACAATCGACCGATCCTGATNNNNNN202300.17629798629877935No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGAGTNNNNN194290.16931752722684051No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCNN188880.16460288508212279No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGNNNNN187730.16360069682585193No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGANNNN176280.15362238766558983No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGANN165360.14410595657126127No Hit
GCTCTGCTAGGCGGACTACTTGCGTGCCTNNNNNN148240.1291864235735593No Hit
TTTTCCACGTTCTAGCATTCAAGGTCCCANNNNNN148210.12916027953209136No Hit
AAAGAGGTGCACAATCGACCGATCCTGANNNNNNN145950.12719076174150687No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGNNN142580.1242539144166088No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCNNNNNNN140750.1226591278870647No Hit
CGGGCGTGCTTGTGGACTGCTTGGTGGGGCNNNNN139340.12143035793807173No Hit
CAATATAGATGGATACGAATAAGGCGNNNNNNNNN137650.11995757693537804No Hit
ACTGCCAGCTGGGACTGAGGACTGCGACGTNNNNN136620.1190599648449789No Hit
CAATATAGATGGATACGAATAAGGCGTNNNNNNNN130420.11365686294160554No Hit
TGCTCTGCTAGGCGGACTACTTGCGTGCCTNNNNN129500.11285511233658885No Hit
GGGCGTGCTTGTGGACTGCTTGGTGGGGCNNNNNN128560.11203593237059353No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTTNNNNNNN122190.10648468089890187No Hit
TTGTGGCGTCGCTGAACCATAGCAGGCTNNNNNNN121020.10546506328165237No Hit
AGAGGTGCACAATCGACCGATCCTGATNNNNNNNN120960.1054127751987165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)