FastQCFastQC Report
Thu 29 Jun 2023
ERR4164647_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4164647_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85150130
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGGTGCACAATCGACCGATCCTGAAGANNNNN1479524417.375480225338467No Hit
AGAGGTGCACAATCGACCGATCCTGAAGANNNNNN40191304.720051513720531No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN28696323.370085283486943No Hit
AATATAGATGGATACGAATAAGGCGTCAGANNNNN22634782.658220251689575No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAATAN21178342.4871764728955785No Hit
AGATGGATACGAATAAGGCGTCAGANNNNNNNNNN20413662.3973727344867237No Hit
AAGAGGTGCACAATCGACCGATCCTGAGANNNNNN18402872.1612262952505183No Hit
CAATATAGATGGATACGAATAAGGCGTCAGANNNN16554651.9441720171184709No Hit
ATAGATGGATACGAATAAGGCGTCAGANNNNNNNN13166981.5463252962737697No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAN6523660.7661362349065116No Hit
TTGCTCGAATATATTAGCATGGAATAGANNNNNNN6317080.7418755555628629No Hit
ATATAGATGGATACGAATAAGGCGTCAGANNNNNN6061880.7119049612725195No Hit
AGATGGATACGAATAAGGCGTCCAGANNNNNNNNN5251450.6167283596631032No Hit
CAAGAGGTGCACAATCGACCGATCCTGAAGANNNN5086600.5973684361961632No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGNNNNNN4871890.5721529726378574No Hit
AGAGGTGCACAATCGACCGATCCTGAGANNNNNNN3319620.38985495383271873No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGAN2938250.3450670010721064No Hit
AAGAGGTGCACAATCGACCGATAGANNNNNNNNNN2785170.3270893420832123No Hit
TTGCTCGAATATATTAGCATGGAATAATAGAAAGN2617670.30741820358935446No Hit
CAATATAGATGGATACGAATAAGGCGTCCAGANNN2461750.2891070160433108No Hit
CAATATAGATGGATACGAATAGANNNNNNNNNNNN2419460.2841404939722347No Hit
AATATAGATGGATACGAATAAGGCGTCCAGANNNN2349290.27589975493871827No Hit
TTGCTCGAATATATTAGCATGGAATAATAGANNNN2268140.2663695287370671No Hit
ATAGATGGATACGAATAAGGCGTCCAGANNNNNNN1949650.2289661800868654No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTTAGN1870220.21963795005362882No Hit
TATAGATGGATACGAATAAGGCGTCAGANNNNNNN1858990.21831910297729434No Hit
AAGAGGTGCACAATCGACCGATCCTGATAGANNNN1765720.2073655084261175No Hit
CAAGAGGTGCACAATCGACCGATCCTGAGANNNNN1745480.2049885302582627No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAGANNN1465500.17210778186715628No Hit
TGCTCGAATATATTAGCATGGAATAATAGAATAGN1439760.16908488571890612No Hit
AATATAGATGGATACGAATAGANNNNNNNNNNNNN1408670.1654336875351805No Hit
AGAGGTGCACAATCGACCGATCCTGAAGNNNNNNN1364820.16028395963693773No Hit
GAGGTGCACAATCGACCGATCCTGAAGANNNNNNN1326350.15576605696315438No Hit
AAGAGGTGCACAATCGACCGATCAGANNNNNNNNN1188580.1395863987524153No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCAGN1163430.13663279198751663No Hit
ATATTAGCATGGAATAATAGAATAGANNNNNNNNN1072620.1259680989330257No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAGAN969470.11385420080979325No Hit
ACAATATAGATGGATACGAATAAGGCGTCAGANNN951630.11175907776065638No Hit
ATTAGCATGGAATAATAGAATAGANNNNNNNNNNN911070.10699572625432281No Hit
AGAGGTGCACAATCGACCGATCCTGATAGANNNNN905850.10638269137111123No Hit
AATATAGATGGATACGAATAAGGCGTCAGNNNNNN903850.10614781210551293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)