FastQCFastQC Report
Mon 26 Jun 2023
ERR4164648_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4164648_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62562746
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGATATAGTGTAACGGCTATCACATCACGAGAN801415412.809786194487053No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN42642296.815923648875642No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAGANNN15346762.4530189259915156No Hit
AAGAGGTGCACAATCGACCGATCCTGAAGANNNNN11438441.8283148888637337No Hit
CCGATATAGTGTAACGGCTATCACATCACGAGANN9368181.497405500711238No Hit
GTCTAGTCGGTTATGGCATCTGCTAGANNNNNNNN9365861.4970346729985284No Hit
TCCGATGTAGTGTAACGGCTATCACATCACGAGAN8571691.370094912394031No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGAN6891781.1015788853002073No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGAGN5232050.8362884199488302No Hit
TCGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGAN4845210.7744560956451624No Hit
TCCGATATAGTGTAACGGCTATCACATCACGCAGN4713570.75341481973953No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAAGN4581590.7323191983932419No Hit
GTCTAGTCGGTTATGGCATCTGCAGANNNNNNNNN4140810.6618651297690802No Hit
CAAGAGGTGCACAATCGACCGATCCTGAAGANNNN3522990.563113070516438No Hit
TCCGATATAGTGTAACGGCTATCACATCACGAGNN3164280.5057770322293718No Hit
TCCGATATAGTGTAACGGCTATCACATCAGANNNN3028350.48405004473428964No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAAGAN2907500.4647334373718187No Hit
CCGTGATAGTTTAATGGTCAGAATGGGCAGANNNN2573840.41140137934482607No Hit
GTTTAGTGGTAAAATCCAACGTAGANNNNNNNNNN2403580.38418710073883267No Hit
TCCGATATAGTGTAACGGCTATCACATCACAGANN2351350.37583868201693066No Hit
TCCGTGATAGTTTAATGGTCAGAATGAGANNNNNN2277680.36406330374309337No Hit
GCCAGATCGGGGTTCAATTCCCCGTCGCGGAGANN2191550.3502963249087564No Hit
TCCTATAGTGTAGTGGTTATCACTTTCGGAGANNN2049350.3275671435521708No Hit
CGTTGGGCCCCCGGTTCGATTCCGGGCTTGCGCAN1796020.2870749950777416No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGAGANNNN1758080.281010683258692No Hit
AGAGGTGCACAATCGACCGATCCTGAAGANNNNNN1737970.2777963102834393No Hit
AAGAGGTGCACAATCGACCGATCCTGAGANNNNNN1640190.2621672009089882No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAGANNNNN1595260.2549856107658702No Hit
TGCCAGATCGGGGTTCAATTCCCCGTCGCGGAGAN1490290.2382072551610826No Hit
TGGGGACACGGGTTCGATTCTCGTACCGCAGANNN1437160.22971498086097436No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCGAGANN1370650.21908405363153335No Hit
AGATCGGGGTTCAATTCCCCGTCGCGGAAGANNNN1321280.2111927759692645No Hit
CCGATATAGTGTAACGGCTATCACATCACGCAGAN1266640.20245914397683246No Hit
GTCTAGTGGTATGATTCTCGCAGANNNNNNNNNNN1230780.1967272983829706No Hit
TTTCCACGTTCTAGCATTCAAGGTCCCAAGANNNN1082400.17301030872270215No Hit
AGTGTAACGGCTATCACATCACGAGANNNNNNNNN1064870.17020832173830733No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCAAGAN1032750.16507427599165803No Hit
TTTCCACGTTCTAGCATTCAAGGTCCAGANNNNNN1021910.16334161547192957No Hit
GTGGAGACCGGGGTTCGACTCCCCGTATCGGAAGN1019050.16288447441229642No Hit
CGATGAAACCCTGGTTCGATTCTAGGAGATGAGAN998660.15962534636826842No Hit
CGTGGAGACCGGGGTTCGACTCCCCGTATCGGAGN988350.1579774008001503No Hit
GTTTAGTGGTAAAATCCAACGAGANNNNNNNNNNN914460.14616685782941816No Hit
TCGGTGCCCGAGTTGTAATTTGGAGAGGGCAGANN869770.13902362917382174No Hit
CCGATGTAGTGTAACGGCTATCACATCACGAGANN850900.13600745721743096No Hit
ATAGTGTAACGGCTATCACATCACGAGANNNNNNN761390.1217002207671639No Hit
CGATGAAACCCTGGTTCGATTCTAGGAGAAGANNN759490.1213965256576174No Hit
TTCCACGTTCTAGCATTCAAGGTCCCAGANNNNNN738120.11798075487287595No Hit
TCCGGACAACCCCGGTTCGAATCCGGGTAGGAGAN710040.11349246083284133No Hit
AGTTTCTTTACTTATTCAATGAAGCGGAGAGANNN695430.11115720527996005No Hit
TTCCACGTTCTAGCATTCAAGGTCCCAAGANNNNN693320.1108199438688321No Hit
TGCCCGCGCTGGTTCAAATCCTGCTGGTGAGANNN690780.11041395145922783No Hit
ATATAGTGTAACGGCTATCACATCACGAGANNNNN684970.1094852837821409No Hit
TCCGATATAGTGTAACGGCTATCAGANNNNNNNNN677040.10821775629861259No Hit
TCCGTGATAGTTTAATGGTCAGAATGGGCAGNNNN653320.10442636261522154No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)