FastQCFastQC Report
Fri 23 Jun 2023
ERR4342736_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4342736_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22863725
Total Bases1.7 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC7449303.2581305102296323No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA6218412.719771165897071Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGG5305252.3203786784524394No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAG4145301.8130466492227315No Hit
CCTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3702581.619412409832606Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC3045991.33223698238148No Hit
CCTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC2202440.9632901025532804No Hit
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2072840.9066064256808547No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA1524820.6669166988318833No Hit
TAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1447820.6332388969863835No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA1326160.5800279700704938No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1250450.5469143807494186No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1172090.5126417501959982No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC1156240.5057093715044245No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA1089230.4764009364178409No Hit
ATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1030150.4505608775472938No Hit
TCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA970390.4244234043227864No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGC959220.41953793618493923No Hit
CAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT950440.41569779202645235Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA942550.41224691077241354No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGG892090.39017701621236256No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACC840370.36755603034938533No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC822440.35971391363393324No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGC810860.35464912213561No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCATC759440.3321593484876152No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCA749910.32799117379167214No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAG740090.32369616062124607No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGG732920.3205601886831651No Hit
TTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA728390.3185788842369299Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC705570.3085980084172636No Hit
CTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC693010.30310459034999765No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGCACTGTAGGCACCATCAATAG651520.2849579410179225No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACC615110.26903315185955046No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCA603400.2639115017347348No Hit
ATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA564750.2470069947044937No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAAT548200.23976845417796092No Hit
TAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC531660.2325342873919276No Hit
CCTTTGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT526610.23032554843972272No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT510680.22335817982415376No Hit
AGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAG509460.2228245834832251No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA502260.21967549032364586No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA491180.21482938585029343No Hit
TCCTGGGCTACACACGTGCTACAATGGCTGGTACAAAGCTGTAGGCACCA485750.21245444475911077No Hit
TTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG475600.20801509815220395Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG471550.20624373324994066Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGCA461780.2019705887820117No Hit
TCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCGGAA452120.19774555545957626No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTACGCACTGTAGGCACCATCAAT445580.19488512917295847No Hit
GTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCTC441230.1929825520557127No Hit
ACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCAC434440.19001278225660956No Hit
TCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA433070.1894135798081896No Hit
AGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG432890.18933485247920012No Hit
GACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG425640.18616389061712385No Hit
CGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC424590.1857046478646852No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATC412230.18029870460740757No Hit
TGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC411570.18001003773444615Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATCTGTAGGCACCATCAA410490.17953767376050928No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT401440.1755794386085382No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT397090.17367686149129244No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA393450.17208481994950517No Hit
ATACCCTAGTAGTCCACGCCGTAAACGATGAGTACTAGGTGTTGGGGTCT391630.17128879917861153No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC390440.17076832405918108No Hit
TTAAATCAGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGAT387580.16951743427634824No Hit
ATGCATGTCTAAGTACGCACTGTAGGCACCATCAATAGATCGGAAGAGCA386170.16890073686593063No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT383030.16752738234911416No Hit
CTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCAT382950.16749239242511882No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG380180.16628086630678074No Hit
TGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA378280.16544985561189177Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGC370190.16191149954786457No Hit
AAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG367400.16069122594852764Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGA366530.16031071052507848No Hit
GCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC365170.15971588181715798No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAGG360440.15764710256093442No Hit
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGAG345570.15114335043830346No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC328200.14354616319081864Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATCTCAGCGCCGCAGCTAACGCATTAAGTACTCCGCCTGAGTACTGTAGG319610.13978912010182068No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATCGGA314920.1377378358075948No Hit
CTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT314120.13738793656764153Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT303810.1328786101127441No Hit
TCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAA291380.12744205067197056No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATCA290930.12724523234949686No Hit
AGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGC290480.12704841402702316No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGG287520.12575378683919614No Hit
AGGAATAATGGAATCTGTAGGCACCATCAATAGATCGGAAGAGCACACGT287430.1257144231747014No Hit
CTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA279790.12237288543314792No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGCCTGTAGGCACCATCAATAGA274610.12010728785445066No Hit
CGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGC273950.11981862098148924No Hit
TTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAG271530.11876017578063068No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT271390.11869894341363886No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA267900.11717250797934281No Hit
TCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC262160.11466198093267829No Hit
GTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCG258820.1132011516058735No Hit
TTTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAG256190.11205085785452722No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAG255560.11177531220306404No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGCACCATCAATAGATCG252980.1106468871542148No Hit
AAAGCGAATGATTAGAGGTCTGTAGGCACCATCAATAGATCGGAAGAGCA252870.11059877600872124No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG252800.11056815982522533No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATCTGTAGGCACCATCA249650.10919043156790943No Hit
TTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTACTGT249580.10915981538441352No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGG248500.10868745141047664No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGCACCATCAATAGATC245500.10737532926065198No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATCAATAGATCGG245230.10725723826716775No Hit
AATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGA244100.1067630055907338No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA241770.10574392405436996No Hit
AAAGCGAATGATTAGAGGTCCTGTAGGCACCATCAATAGATCGGAAGAGC239900.10492603458097927No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATCAATAGATCG238580.1043487008350564No Hit
TGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATC237580.10391132678511485No Hit
AGCTTTCGCCGAATCCCGGGGCCGAGGAAGCCAGACTGTAGGCACCATCA231060.1010596479794959No Hit
AACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAG230000.10059603148655785No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)