FastQCFastQC Report
Thu 29 Jun 2023
ERR4342737_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4342737_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69720356
Total Bases5.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAG30447254.367053145855997No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC18751082.689469916074439No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC15251322.187498870487695No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTA9596931.3764889553920234No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA6252810.8968413758529862No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAG5759500.8260858564749727No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCA5199380.7457477698478763No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTACGCACTGTAGGCACCATCAAT5185490.743755525287335No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC4709260.6754497925971578No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGG4380370.6282770558429163No Hit
ATCTCAGCGCCGCAGCTAACGCATTAAGTACTCCGCCTGAGTACTGTAGG4355090.6246511420567044No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA4212270.6041664503262146No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGG4070300.5838036742095809No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAG3818170.5476406345372075No Hit
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA3726510.5344938284595104Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAT3518640.5046790065156868No Hit
ATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA3485940.4999888411355788No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT3435310.4927269734537787No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC3375890.4842043548945734No Hit
ATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC3278440.470227088341316No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCG3024360.43378435990774344No Hit
TCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2929860.4202302122496334No Hit
TCGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGAAAATCCGGGGGAGAG2677360.38401410342769904No Hit
TCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCGGAA2608340.37411455558259055No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA2469870.3542537849347757No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAAT2260490.32422238348869015No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCTGTAGGCACCATC2131400.30570698749730996No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACTGTAGGC2093800.3003140144608556No Hit
TCCTGGGCTACACACGTGCTACAATGGCTGGTACAAAGCTGTAGGCACCA2042610.29297182590404447No Hit
ATACCCTAGTAGTCCACGCCGTAAACGATGAGTACTAGGTGTTGGGGTCT1902890.2729317675887943No Hit
TATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCA1887950.27078892138760735No Hit
TAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA1868720.26803075991178243No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAG1865660.26759186370190075No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGCACTGTAGGCACCATCAATAG1847500.26498717246940046No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACC1799530.2581068289439027No Hit
TGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG1799530.2581068289439027No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGG1723060.24713872660087968No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1721410.24690206687986502No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1704310.2444494115893499No Hit
ATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAAT1696740.2433636454753616No Hit
AAAAGATTAAGCCATGCATGTCTAAGTACGCACTGTAGGCACCATCAATA1667350.23914823383862238No Hit
ATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC1638680.23503609189832594No Hit
TTGCCCTCGGCCGATCGAAAGGGAGTCGGGTTCAGATCCCCGAATCCGGA1635950.2346445276326472No Hit
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA1609230.23081207445354981No Hit
ATGCATGTCTAAGTACGCACTGTAGGCACCATCAATAGATCGGAAGAGCA1524690.21868649092956438No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACTGTAG1455130.20870949081212378No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCTGTAGGCACCAT1424640.2043363060280415No Hit
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAG1411480.20244876546528248No Hit
CTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTA1393340.19984694283546114No Hit
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC1267430.1817876546700364No Hit
AGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAG1228740.1762383427875784No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACC1213210.17401087280736202No Hit
TCTTCCCGGAGTCGGGTTGCTTGGGAATGCAGCCCAAAGCGGGTGGTACT1194120.17127279155028985No Hit
TCCACGCCGTAAACGATGAGTACTAGGTGTTGGGGTCTGTAGGCACCATC1165250.16713196358320373No Hit
TCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGCTGTAGGCAC1157400.16600603703170994No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGG1155030.16566610761425257No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAG1154070.16552841468566226No Hit
AGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCA1128420.1618494317498895No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAA1079350.15481131507704865No Hit
ACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCAT1063450.1525307759472714No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGC1043840.14971811102054614No Hit
TTTTGGTGACTCTAGATAACCTCGGGCCGATCGCACCTGTAGGCACCATC1043820.14971524241786716No Hit
ATTAAATCAGTTATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATA1041150.14933228396022533No Hit
CAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1020500.14637045169419388Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT1004110.144019631798782Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA998820.1432608863901957No Hit
CCCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTA994290.14261114788341012No Hit
TCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAA984220.1411668064345512No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA975940.13997920492545965No Hit
ATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGCACCATCAATAGATCG970070.13913727003918339No Hit
TTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAG965840.13853056057258226No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTTGGGG960290.13773452332916947No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGCACCATCAATAGATC959700.13764989955013998No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTACGCCTGTAGGCACCATCAATA957250.13729849572196678No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG912580.13089147163849824No Hit
ACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCG910460.13058739975452793No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC898940.12893508461144404No Hit
TCGTCGCGGCGTGGGAAATGTGGCGTACGGAAGACCCACTCCCCGGCGCC870350.12483441708186344No Hit
ACTACTCTCATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCAC840800.12059605662369251No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT820710.11771454523267207No Hit
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC813050.11661587040662845No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA811990.11646383446464328No Hit
ATCTCAGCGCCGCAGCTAACGCATTAAGTACTCCGCCTGAGTAGCTGTAG807660.11584278198464736No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC806020.11560755656497221Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TAGCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGC799890.11472832984386941No Hit
TCAGGATAGCTGGCGCTCTCGCTCTTTTTCTGTAGGCACCATCAATAGAT798510.11453039625902081No Hit
TTTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC791270.11349196208923545No Hit
TTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGG788440.1130860548101619No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGCACGGCTGTAGGCACCATCAA774450.11107946723622582No Hit
TAGAGCTAATACATGCCGACGGGCGCTGACCCCCTGTAGGCACCATCAAT766940.11000230693027442No Hit
TAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAG753430.1080645658206335No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTCTGTAGGCACCATC752840.10797994204160404No Hit
TTTTGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC750330.10761993240539391No Hit
TGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG738850.10597335446766797No Hit
TTGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGA734930.10541110834259078No Hit
TTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATA733820.10525190089390823No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCAC718820.10310044888468442No Hit
TCTACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGG716350.10274617645383222No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGCACCATCAATAG707260.10144239653624258No Hit
AAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCTGTAGGCACCATCA704360.10102644914779266No Hit
TCGACGAAGCCGAGCGCACGGGGTCGGCGGCGACTGTAGGCACCATCAAT699520.10033224729948309No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)