FastQCFastQC Report
Mon 26 Jun 2023
ERR4342738_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4342738_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37412107
Total Bases2.8 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA29794667.963908581786104Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCAC8019702.143610890453189No Hit
CAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT4183931.1183358371128362Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC3699200.9887708275826326No Hit
GCGGTCGGCGTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT3620650.967774950499313Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACA3585530.9583876150038809No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGG3278090.8762110083775821No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAG3048100.8147362563674909No Hit
TTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGC2693180.7198685708880284No Hit
TCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2531970.6767782418669978Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC2425000.6481858934061104Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCAC2416440.645897864025675No Hit
CTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCAC2251110.6017062872187338No Hit
TCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACA2123600.5676237374174087No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCAC2022980.5407287004712138No Hit
AGTTATGGTTCCTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGA1855370.4959276952778949No Hit
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACCA1814090.48489383396663543No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCA1813730.48479760843194425No Hit
ACCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA1691610.4521557687194683No Hit
ATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCA1656900.4428780234163235No Hit
TGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAAC1490880.39850201433455756Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACC1486070.39721633427382214No Hit
ATGCATGTCTAAGTACGCACTGTAGGCACCATCAATAGATCGGAAGAGCA1464350.3914107270141187No Hit
ATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATA1399130.37397786764589336No Hit
TGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA1309750.3500872057272797Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
AGTTATGGTTCCTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAG1241750.33191127139671656No Hit
CCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCAC1144340.3058742454681849No Hit
ACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAGC1074420.28718510828593535No Hit
CGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACAC1019990.27263634202692727No Hit
GACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCACACG1009140.26973621132859477No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGCACTGTAGGCACCATCAATAG969020.25901241007356257No Hit
TTCTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCA940070.2512742733254772No Hit
AGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACACG926580.2476684887060758No Hit
TAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACAC917520.2452468127496802No Hit
AGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGA908160.24274494884770859No Hit
AATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA898210.2400853819861041Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG878340.2347742670574528Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTCA870970.2328043165278021Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGGC843330.22541633380873202No Hit
TCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGCTGTAGG827010.22105410956939686No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTACGCACTGTAGGCACCATCAAT805890.2154088782008455No Hit
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGG794230.2122922400494578No Hit
TTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA770610.20597877580110632Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATC741240.19812837592921456No Hit
ATTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGCTGTAGGCACC738380.19736391751472324No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGCTGTAGGCA734670.19637225992109988No Hit
TTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT727460.19444507629575633Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAG709620.18967656646550274No Hit
CTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG694960.18575804885835487Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC674680.18033734373741633Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGA670920.17933232148619696No Hit
TCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACCATCAATAGATCGGAA658550.176025905196946No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCTGTAGGCACCATCAATAGATCGGA656660.17552072113981712No Hit
CTAGAGCTAATACATGCCTGTAGGCACCATCAATAGATCGGAAGAGCACA656280.1754191497420875No Hit
TGGATAACTGTGGTAATTCTAGAGCTAATACATGCCCTGTAGGCACCATC647720.17313112036165193No Hit
TATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAAT647580.1730936993203831No Hit
ATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGC644570.17228914693310377No Hit
GTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGT642650.17177594408141728Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TCGACTGCATAATTTGTGGTAGTGGGGCTGTAGGCACCATCAATAGATCG636100.17002517393634098No Hit
CACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAGAG619340.165545340710161No Hit
GCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCAGTC615200.16443874706121203Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCTGTAGGCACC611970.1635753901805103No Hit
GCGTTTACTTTGAAAAAATTAGAGTGTTCAAAGCAGGCCCCTGTAGGCAC605650.1618860974603756No Hit
AAGCCATGCATGTCTAAGTACGCACTGTAGGCACCATCAATAGATCGGAA603760.1613809134032467No Hit
TCGACACAAGGGTTTGCTGTAGGCACCATCAATAGATCGGAAGAGCACAC602270.16098264660688583No Hit
AAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT594590.1589298352001399Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTAAACTCCAGTCA593910.15874807585683426Illumina Multiplexing PCR Primer 2.01 (96% over 33bp)
ATGCATGTCTAAGTACGCACGGCTGTAGGCACCATCAATAGATCGGAAGA593460.15862779393847026No Hit
AGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGC585700.15655359907957067No Hit
AAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG582080.15558599786962013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCA579850.15498993414083842No Hit
TCTAGAGCTAATACATGCCCTGTAGGCACCATCAATAGATCGGAAGAGCA576470.15408648328734867No Hit
CTTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT562910.15046198814731285Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAAGATTAAGCCATGCATGTCTAAGTACGCACGGCTGTAGGCACCATCAA559570.1495692290198999No Hit
TCCTGCGGAAGGATCATTACTGTAGGCACCATCAATAGATCGGAAGAGCA557780.14909077427796302No Hit
TTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGAAG555930.14859628194691094No Hit
GACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGC547640.14638042171749374No Hit
TTTGGTCGCTCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTG546770.1461478766753233Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCTGTAGG524940.14031286716890873No Hit
TGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGA518540.1386021909966204Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCGCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTA513340.13721226660663619No Hit
TTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAGATCGGA511220.1366456051245657No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAA502440.1342987712507077No Hit
ATGCATGTCTAAGTACGCCTGTAGGCACCATCAATAGATCGGAAGAGCAC476870.12746408535611214No Hit
TCGACGTACGCAGTTTTATCCGGTAAAGCGAATGATTAGAGGTCCTGTAG476430.1273464763692673No Hit
ATCTCAGCGCCGCAGCTAACGCATTAAGTACTCCGCCTGAGTACTGTAGG471170.12594051439016787No Hit
AGTTATGGTTCCTTTGGTCGCTCGCTGTAGGCACCATCAATAGATCGGAA468410.12520278529086853No Hit
CGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATCAATAGATCGGAAG467680.1250076612899669No Hit
TGGGGAAGGTGGCTCGGGGGGCTGTAGGCACCATCAATAGATCGGAAGAG465110.1243207178895324No Hit
CCTTTGGTCGCTCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCT456040.12189636900161757Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGTACGCACTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAA451320.12063474532455495Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAAT442570.11829593024525456No Hit
CCGGGGTCCGGTGCGGAGAGCCGTTCCTGTAGGCACCATCAATAGATCGG441030.11788429879129769No Hit
TCATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAAT438660.1172508140212472No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCTGTAGGCACCATCAATAGATCG436500.11667346081309989No Hit
TGGCCCGAGGTGGGATCCCGAGGCCTGTAGGCACCATCAATAGATCGGAA432490.1156016152739005No Hit
ATCGTTTTTTCACTGACCCGGTGAGGCGGGGCTGTAGGCACCATCAATAG429080.11469014562585314No Hit
TCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCTGTAGGCACCATC427980.11439612315874109No Hit
TCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTC427540.11427851417189629No Hit
CGGTAAAGCGAATGATTAGAGGTCCTGTAGGCACCATCAATAGATCGGAA425520.11373858200501778No Hit
CTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAAGAGCAC420020.11226846966945754No Hit
ACCCCGAGTGTTACAGCCCTCCGGCTGTAGGCACCATCAATAGATCGGAA418230.11179001492752066No Hit
TTGATCATCGACACTTCGAACGCACTTGCGGCCCCGGGTCTGTAGGCACC416270.11126612034975736No Hit
TGTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGG412770.11033059431803721No Hit
GCTGCGATCTATTGAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGC407150.10882840680424655No Hit
TCGGGCCGATCGCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACG405440.10837133551446328No Hit
TCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGATCGGAA404570.10813879047229284No Hit
TACTTGGATAACTGTGGTAATTCTAGAGCTAATACATGCCGACGGGCGCT401000.10718455391993827No Hit
AAGCCATGCATGTCTAAGTACGCACGGCTGTAGGCACCATCAATAGATCG399520.1067889600550966No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGACTGTAGGCACCATCAATA396650.10602182870908608No Hit
TAGAGTGTTCAAAGCAGGCCCCTGTAGGCACCATCAATAGATCGGAAGAG388090.10373379932865048No Hit
AAAGATTAAGCCATGCATGTCTAAGTACGCCTGTAGGCACCATCAATAGA384500.10277421691325753No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAACTGTAGGCACCATCAAT383320.10245881099399186No Hit
TATCCGGTAAAGCGAATGATTAGAGGTCCTGTAGGCACCATCAATAGATC381550.10198570211509338No Hit
GCACCTGTAGGCACCATCAATAGATCGGAAGAGCACACGTCTGAACTCCA377360.10086574380854839Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAAAGTCAGCCCTCGACACAAGGGTTTGTCTGTAGGCACCATCAATAGAT374150.10000773279088505No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)