FastQCFastQC Report
Sat 24 Jun 2023
ERR4777060.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4777060.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11345850
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAAGATCGGAAGAGCACACGTCT156636713.805638184886984Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG139652412.308676740834755Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACAGATCGGAAGAGCACACGTC129953911.453870798573929No Hit
AGGCACGTAGCTCAGTTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT1803991.5899998677930696Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTAGATCGGAAGAGCACACGTCT1367341.2051454937267811Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGAGATCGGAAGAGCACA1060120.9343680729077151No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGAGATCGGAAGAGCACACGTCTG1007240.8877607230837707Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAAGGCCGAGAGCTGATGACGAGTCAGATCGGAAGAGCACACGTCTGAA908620.8008390733175565Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAAGATCGGAAGAGCACACGTCT872050.7686070237135164Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGAGATCGGAAGAGCACACGTC647480.5706756214827448No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT645550.568974558979715No Hit
GCCCAGGTGGCGGAATTGGTAGACGCACTAGATCGGAAGAGCACACGTCT642300.5661100754901572Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAGATCGGAAGAGCACACGT635260.5599051635620073No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTG592320.5220587263184336Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGTAGATCGGAAGAGCACACGTCTG575850.5075424053728896Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGTGGTGGAATTGGTAGACACGCAGATCGGAAGAGCACACGTCTG569230.5017076728495441Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTAAGATCGGAAGAGCACACGTC560590.49409255366499644No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTAGATCGGAAGAGCACACGTCT532410.4692552783616917Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCAGGTGGCGGAATTGGTAGACGCACAGATCGGAAGAGCACACGTCTG497890.43883005680491105Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAGATCGGAAGAGCACAC496310.4374374771392183No Hit
GGAGAGGTGGCCGAGTGGCCGAAGGCGCTAGATCGGAAGAGCACACGTCT492330.433929586588929Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATAGATCGGAAGAGCACAC469080.41343751239439974No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT401830.3541647386489333No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTAGATCGGAAGAGCACACG398900.351582296610655No Hit
GAACTGAAGAGTTTGATCATGGCTCAGAAGATCGGAAGAGCACACGTCTG342680.30203113913898033Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCCGCGATAGCTCAGTCGGTAGAGCAAATGACTGAGATCGGAAGAGCACA322580.2843154104804841No Hit
GCCTCGGTGGCGGAATCGGTAGACGCGGCAGATCGGAAGAGCACACGTCT309780.27303375242930233Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGATTAGCTCAGTCGGTAGAGCAGCTAGATCGGAAGAGCACACGTCT302950.26701393020355463Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGATTAGCTCAGTCGGTAGAGCAGCAGATCGGAAGAGCACACGTCTG292890.258147252078954Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAAGATCGGAAGAGCACACG292380.2576977485159772No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGAGATCGGAAGAGCACACGTC289600.2552475134079862No Hit
GGTGAGGTGTCCGAGTGGTTGAAGGAGCAGATCGGAAGAGCACACGTCTG286900.252867788662815Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCTCATGTAGCTCAGTTGGTAGAGCACACAGATCGGAAGAGCACACGTCT282980.24941278088464067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCATAGATCGGAAGAGCACACGTC273210.2408017028252621No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTGAGATCGGAAGAGCACACGTC265040.23360083202228127No Hit
GTCCCCTTCGTCTAGTGGCCTAGGACACCGAGATCGGAAGAGCACACGTC248430.21896111794180254No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTGA245310.21621121379182695Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGGCAGATCGGAAGAGCA240290.21178668852487914No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAAGATCGGAAGAGCACA229650.20240881026983437No Hit
GCTCATGTAGCTCAGTTGGTAGAGCACACCAGATCGGAAGAGCACACGTC211350.1862795647747855No Hit
TCAAGGCCGAGAGCTGATGACGAGTCGTAGATCGGAAGAGCACACGTCTG206300.1818285981217802Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGATCAGATCGGAAGAGCA196690.17335854078804144No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCAGATCGGAAGAGCACACGT193980.17097000224751782No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAAGATCGGAAGAGCACACG189530.16704786331566168No Hit
GGGTCTGCAGCTCAGTTGGTTAGAGCGCACAGATCGGAAGAGCACACGTC181360.15984699251268084No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTGATCCAGATCGGAAGAGC177520.15646249509732635No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGAAGATCGGAAGAGCACACGTCTG173130.1525932389375851Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCAGATCGGAAGAGCACACGTCTGAA165080.1454981336788341Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCCCTTCGTCTAGTGGCCTAGGACACCAGATCGGAAGAGCACACGTCT164930.14536592674854681Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGAGGCGGCCGAGTGGCCGAAGGCGCTAGATCGGAAGAGCACACGTCT163100.14375300219904194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACGCCGGTTCGATTCCGACCTCAGCCTCCAAGATCGGAAGAGCACAC162850.1435326573152298No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACAGATCGGAAGAGCACA162380.14311840893366298No Hit
GGTGAGGCGTCCGAGTGGTTGAAGGAGCAGATCGGAAGAGCACACGTCTG161560.14239567771475914Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGAGATGCCGGAGTGGTCGAACGGGACGGATTAGATCGGAAGAGCACA153990.13572363463292747No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGAGATCGGAAGAGCACACGT152360.13428698599047229No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATCGGAAGAGCACACGTCTGAAC146210.12886650184869358Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTACAGCTCAGCTGGGAGAGCGCTTGCATGAGATCGGAAGAGCACA146200.1288576880533411No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAAAGATCGGAAGAGCACAC144470.12733290145736106No Hit
CCGTGTCGGCGGTTCGATTCCGTCCCTGGGCACCAAGATCGGAAGAGCAC142240.12536742509375676No Hit
GGAGAGGTGGCCGAGTGGCCGAAGGCGCAGATCGGAAGAGCACACGTCTG142190.12532335611699433Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGCAGCTCAGTTGGTTAGAGCGCAAGATCGGAAGAGCACACGTCT140860.12415112133511372Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGAAGATCGGAAGAGCACACG140470.12380738331636677No Hit
GCTCATGTAGCTCAGTTGGTAGAGCACACCCTAGATCGGAAGAGCACACG138510.12207987942727958No Hit
AGGCACGTAGCTCAGTTGGTTAGAGCACCAAGATCGGAAGAGCACACGTC136880.1206432307848244No Hit
CGCGGGGTGGAGCAGTCTGGTAGCTCGTCGAGATCGGAAGAGCACACGTC136300.12013203065438023No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGAGATCGGAAGAGCACACGTCTGA131500.1159014088851871Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGGGATTCCCGAGTGGCCAAAGGGATCAGATCGGAAGAGCACACGTCT131110.11555767086644014Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGCAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG131010.1154695329129153Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTAGATCGGAAGAGCACACGTCTG130630.11513460868952084Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGAGATGCCGGAGTGGTCGAACGGGAAGATCGGAAGAGCACACGTCTG129660.11427967054032971Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGATAGATCGGAAGAGCACAC129110.113794911795943No Hit
GCGGACGTGGTGGAATTGGTAGACACACTGAGATCGGAAGAGCACACGTC127750.11259623562800496No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTAGATCGGAAGAGCACACGTC122010.10753711709567816No Hit
GCGGACGTGGTGGAATTGGTAGACACACTAGATCGGAAGAGCACACGTCT117270.10335937809859994Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCCAGGTGGCGGAATTGGTAGACGCACTAAGATCGGAAGAGCACACGTC117130.10323598496366512No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTGATAGATCGGAAGAGCAC114510.10092677058131387No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)