FastQCFastQC Report
Fri 23 Jun 2023
ERR4777061.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4777061.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10553078
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAAGATCGGAAGAGCACACGTCT164572115.594701375276484Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACAGATCGGAAGAGCACACGTC111008510.519063727189357No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG8866988.402268987303989Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTAGATCGGAAGAGCACACGTCT1518741.439144105634394Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AGGCACGTAGCTCAGTTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT1393071.3200603653265899Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAAGGCCGAGAGCTGATGACGAGTCAGATCGGAAGAGCACACGTCTGAA1190311.1279268475036381Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCAGGTGGCGGAATTGGTAGACGCACTAGATCGGAAGAGCACACGTCT1015470.9622500658101836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGAGATCGGAAGAGCACACGTCTG912970.865122005162854Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGGAGATCGGAAGAGCACAC691840.6555812436902295No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGTAGATCGGAAGAGCACACGTCTG644560.610779148983832Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTAGATCGGAAGAGCACACGTCT599420.5680048986655836Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTAAGATCGGAAGAGCACACGTC536390.5082782482987428No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAGATCGGAAGAGCACACGT516950.4898570824549956No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAAGATCGGAAGAGCACACGTCT476080.4511290450046896Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCATAGATCGGAAGAGCACACGTC464290.4399569490531578No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTGAGATCGGAAGAGCACACGTC435850.41300746568915725No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGAGATCGGAAGAGCACACGTC433100.410401590891302No Hit
GGTGAGGTGTCCGAGTGGTTGAAGGAGCAGATCGGAAGAGCACACGTCTG417530.39564760158126383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCAGGTGGCGGAATTGGTAGACGCACAGATCGGAAGAGCACACGTCTG409430.38797211581303576Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGGATTAGCTCAGTCGGTAGAGCAGCTAGATCGGAAGAGCACACGTCT398210.37734014663778664Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTG303730.2878117644918383Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGGGATTCCCGAGTGGCCAAAGGGATCAGATCGGAAGAGCACACGTCT283230.2683861523623724Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGAGATCGGAAGAGCACACGTC273120.25880600901462114No Hit
CTGGCCTTGACATGCTGAGAACTTTCCAGAGATGGAAGATCGGAAGAGCA272240.25797212907930744No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCAGATCGGAAGAGCACACGTCTG270000.2558495256076Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCAGGTGGCGGAATTGGTAGACGCACTAAGATCGGAAGAGCACACGTC252960.2397025777692537No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGAGATCGGAAGAGCACA240790.2281703973002No Hit
AACACATGCAAGTCGAGCGGATGAAGGAGATCGGAAGAGCACACGTCTGA230020.21796484400096353Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TCAAGGCCGAGAGCTGATGACGAGTCGTAGATCGGAAGAGCACACGTCTG223810.2120803049119887Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTGGTGCCTTCGGGAACTCAGACACAGGTAGATCGGAAGAGCACACGTCT221670.21005246052383958Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TTGGTGCCTTCGGGAACTCAGACACAGGAGATCGGAAGAGCACACGTCTG218700.207238115742156Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCTCGGTGGCGGAATCGGTAGACGCGGCAGATCGGAAGAGCACACGTCT210980.1999227144914498Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT205730.19494786260463537No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTAGATCGGAAGAGCACACGTCTG199900.18942340803318236Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGGTGGAGCAGTCTGGTAGCTCGTCGAGATCGGAAGAGCACACGTC193650.18350096531078422No Hit
GTCCCCTTCGTCTAGTGGCCTAGGACACCGAGATCGGAAGAGCACACGTC187760.17791965528919618No Hit
GGAGAGATGCCGGAGTGGTCGAACGGGAAGATCGGAAGAGCACACGTCTG186400.17663093175280237Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ACGGCGGTAACAGGGGTTCGAGTCCCCTAGGAGATCGGAAGAGCACACGT171270.16229388241042092No Hit
AACACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCACACGT170640.16169690018400318No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTGA163430.15486477025944467Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCAGCGGTAGTTCAGTCGGTTAGAATAAGATCGGAAGAGCACACGTCTGA158100.14981411110578355Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTCAAGGCCGAGAGCTGATGACGAGTCAGATCGGAAGAGCACACGTCTGA156230.148042116243242Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
CCGTGTCGGCGGTTCGATTCCGTCCCTGGGCACCAAGATCGGAAGAGCAC152580.1445834096933615No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTAGATCGGAAGAGCACACG151570.14362634294942195No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCAGATCGGAAGAGCA151490.14355053568257525No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGAAGATCGGAAGAGCACACGTCTG150840.14293460163944588Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCAGATCGGAAGAGCACACGTCTGA147440.13971279279846127Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGACGTGGTGGAATTGGTAGACACACTAGATCGGAAGAGCACACGTCT146180.1385188283456258Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTATAGCTCAGCTGGGAGAGCAGATCGGAAGAGCACACGTCTGAAC144690.13710691800060607Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGAGAGATGCCGGAGTGGTCGAACGGGACAGATCGGAAGAGCACACGTCT144370.1368036889332193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGACAGAACCCGGCTTATAGATCGACTCTCAGATCGGAAGAGCACAC144260.1366994539413051No Hit
GCGGACGTGGTGGAATTGGTAGACACACTGAGATCGGAAGAGCACACGTC143890.13634884533213912No Hit
GCTCATGTAGCTCAGTTGGTAGAGCACACAGATCGGAAGAGCACACGTCT141050.1336576873590814Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGGACTCCCGAGTGGCCAAAGGGATCAGATCGGAAGAGCACACGTCT137490.1302842639844034Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGAGGCGTCCGAGTGGTTGAAGGAGCAGATCGGAAGAGCACACGTCTG137460.1302558362593359Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGAGAGATGCCGGAGTGGTCGAACGGGACGGATTAGATCGGAAGAGCACA136650.1294882876825131No Hit
GCCGGATTAGCTCAGTCGGTAGAGCAGCAGATCGGAAGAGCACACGTCTG136170.1290334440814329Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATAGATCGGAAGAGCACAC133530.12653180427549196No Hit
CGCGGGGTGGAGCAGTCTGGTAGCTCGTCAGATCGGAAGAGCACACGTCT132910.12594429795743006Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
CCTCGGTGGCGGAATCGGTAGACGCGGCAGATCGGAAGAGCACACGTCTG127870.12116844014608819Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCAGATCGGAAGAGCACACGTCTGAA127680.12098839788732728Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAGATCGGAAGAGCACAC126330.11970915025928928No Hit
CAAGGCCGAGAGCTGATGACGAGTCAGATCGGAAGAGCACACGTCTGAAC125640.11905531258273652Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
ACGGCGGTAACAGGGGTTCGAGTCCCCTAGGGAGATCGGAAGAGCACACG124660.11812667356386448No Hit
GCTCATGTAGCTCAGTTGGTAGAGCACACCAGATCGGAAGAGCACACGTC122850.116411534151458No Hit
GGCCTAACACATGCAAGTCGAGCGGATGAAGGAGATCGGAAGAGCACACG121170.11481958154767737No Hit
AGGCACGTAGCTCAGTTGGTTAGAGCACTAGATCGGAAGAGCACACGTCT118830.1126022189924115Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCCCTTCGTCTAGTGGCCTAGGACACCAGATCGGAAGAGCACACGTCT118070.11188204995736789Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCGAGATCGGAAGAGCACACGTCT117540.11137982681450852Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCAGATCGGAAGAGCACACGT117420.11126611591423849No Hit
ACCTCAAAAGGGTGTCGGGGGTTCGAATCCAGATCGGAAGAGCACACGTC107180.10156278575786136No Hit
TGGCCTTGACATGCTGAGAACTTTCCAGAGATGGAAGATCGGAAGAGCAC106510.10092789989802027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)