FastQCFastQC Report
Fri 23 Jun 2023
ERR4777062.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4777062.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4982539
Total Bases498.2 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACAGATCGGAAGAGCACACGTC57685511.577531053946592No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG52084410.453385312187221Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAAGATCGGAAGAGCACACGTCT4685539.403900300629859Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAAGGCCGAGAGCTGATGACGAGTCAGATCGGAAGAGCACACGTCTGAA1469302.9488981420918132Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGAGGTGGTGGAATTGGTAGACACGCAGATCGGAAGAGCACACGTCTG1054932.1172538739787083Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTAGATCGGAAGAGCACACGTCT848451.7028466811800167Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGATTAGCTCAGTCGGTAGAGCAGCTAGATCGGAAGAGCACACGTCT449810.9027726626926552Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGAGGTGTCCGAGTGGTTGAAGGAGCAGATCGGAAGAGCACACGTCTG394480.7917248615615453Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAAGATCGGAAGAGCACACGTCT321350.644952302430548Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAAGGCCGAGAGCTGATGACGAGTCGTAGATCGGAAGAGCACACGTCTG321000.6442498493238086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCACGTAGCTCAGTTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT307970.6180985236643406Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGAATAGCTCAGTTGGTAGAGCACGAGATCGGAAGAGCACACGTCTG304060.6102511189576239Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGTAGATCGGAAGAGCACACGTCTG288610.5792428318172723Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTG240070.48182262095690565Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTAGATCGGAAGAGCACACGTCT213750.42899814733010616Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGGATTCCCGAGTGGCCAAAGGGATCAGATCGGAAGAGCACACGTCT204880.4111959785964545Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGAGGCGTCCGAGTGGTTGAAGGAGCAGATCGGAAGAGCACACGTCTG183660.36860725023928564Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATG179350.35995704198200956TruSeq Adapter, Index 11 (100% over 49bp)
GGAGAGATGCCGGAGTGGTCGAACGGGAAGATCGGAAGAGCACACGTCTG176330.35389587517528714Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGAGATCGGAAGAGCACACGTC169900.340990808100047No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCATAGATCGGAAGAGCACACGTC165930.33302298286074633No Hit
TCAAGGCCGAGAGCTGATGACGAGTCGTCAGATCGGAAGAGCACACGTCT163580.32830651200121064Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTAAGATCGGAAGAGCACACGTC161530.32419214380459443No Hit
GGAGAGATGCCGGAGTGGTCGAACGGGACAGATCGGAAGAGCACACGTCT157890.31688663149450513Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGAACGCCGGTTCGATTCCGACCTCAGCCTCCAAGATCGGAAGAGCACAC134900.2707454974261115No Hit
GCCCAGGTGGCGGAATTGGTAGACGCACAGATCGGAAGAGCACACGTCTG129150.25920519638682205Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCAGGTGGCGGAATTGGTAGACGCACTAGATCGGAAGAGCACACGTCT128420.2577400799070514Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGAGGTGGTGGAATTGGTAGACACAGATCGGAAGAGCACACGTCTGAA126930.25474963668121814Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GTCCCCTTCGTCTAGTGGCCTAGGACACCAGATCGGAAGAGCACACGTCT125800.2524817166508882Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GTCCCCTTCGTCTAGTGGCCTAGGACACCGAGATCGGAAGAGCACACGTC125740.2523612961183043No Hit
GCCGAGGTGGTGGAATTGGTAGACACGAGATCGGAAGAGCACACGTCTGA119120.23907489735654855Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGAGATCGGAAGAGCACACGTC114590.2299831471464649No Hit
TCAAGGCCGAGAGCTGATGACGAGTCGAGATCGGAAGAGCACACGTCTGA111880.22454415309142586Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGAGAGGTGGCCGAGTGGCCGAAGGCGCTAGATCGGAAGAGCACACGTCT106330.2134052538274161Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGAGGGACTCCCGAGTGGCCAAAGGGATCAGATCGGAAGAGCACACGTCT105000.21073593202180657Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGAATAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTGA104550.20983277802742736Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCGGACGTGGTGGAATTGGTAGACACACTAGATCGGAAGAGCACACGTCT101660.20403252237463668Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTAGATCGGAAGAGCACACG101310.20333006926789737No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT101110.20292866749261773No Hit
AGGGGCGTCGCCAAGCGGTAAGGCAGCAGATCGGAAGAGCACACGTCTGA95720.19211088964883163Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAGATCGGAAGAGCACACGT94400.1894616379319861No Hit
TGTGGGTGCTTGTGATGTAAGACTGGTGATCAGATCGGAAGAGCACACGT90060.1807512194084181No Hit
AACACATGCAAGTCGAGCGGATGAAGGAGATCGGAAGAGCACACGTCTGA85170.17093694600283107Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTGATTAGCTCAGCTGGGAGAGCATCAGATCGGAAGAGCACACGTCTG82930.1664412461196992Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCAGATCGGAAGAGCACACGTCTGAA80900.16236701810061097Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACCAGATCGGAAGAGCACACGT78260.15706851466691982No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGAAGATCGGAAGAGCACACGTCTG77870.15628578120512454Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAGATCGGAAGAGCACAC76240.15301435673659552No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGAGATCGGAAGAGCACACGT71930.14436414847931947No Hit
GCATCCGTAGCTCAGCTGGATAGAGTACTGAGATCGGAAGAGCACACGTC67600.13567380004451546No Hit
TTCAAGGCCGAGAGCTGATGACGAGTCAGATCGGAAGAGCACACGTCTGA66630.13372700143440924Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGAGATCGGAAGAGCACACGTCTGA65270.13099746936250775Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCGAGGTGGTGGAATTGGTAGACACGTAGATCGGAAGAGCACACGTCTG63870.12818765693555031Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGAGATCGGAAGAGCACA63790.12802709622543848No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTAGATCGGAAGAGCACACGTCTG62520.1254781949524128Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCACTGTTTCGGCTAGGGGGAGATCGGAAGAGCACACGTCTGAACTCCAG62130.12469546149061754Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GCCGAGGTGGTGGAATTGGTAGACACGCCAGATCGGAAGAGCACACGTCT58180.11676777642884481Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCCGGATTAGCTCAGTCGGTAGAGCAGCAGATCGGAAGAGCACACGTCTG56810.11401817426817934Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTGAACGCCGGTTCGATTCCGACCTCAGCCTCCAAGATCGGAAGAGCACA54740.10986366589403515No Hit
GCCGAGGTGGTGGAATTGGTAGACAGATCGGAAGAGCACACGTCTGAACT53410.10719434408842561Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
TGTGGGTGCTTGTGATGTAAGACTGGTGATAGATCGGAAGAGCACACGTC52660.10568908743112698No Hit
CGCGGGGTGGAGCAGTCTGGTAGCTCGTCGAGATCGGAAGAGCACACGTC52380.1051271249457355No Hit
GGAGAGATGCCGGAGTGGTCGAACGGGACGGATTAGATCGGAAGAGCACA51820.10400319997495253No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCAGATCGGAAGAGCACACGTCTGAA51350.1030599058030454Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)