FastQCFastQC Report
Mon 26 Jun 2023
ERR4777063.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4777063.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23541701
Total Bases2.3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACAGATCGGAAGAGCACACGTC2583621.097465302103701No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAAGATCGGAAGAGCACACGTCT2477801.0525152791635575Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG2254000.9574499310818705Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATG1774990.7539769534920183TruSeq Adapter, Index 12 (100% over 49bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAGATCGGAAGAGCACAC1583990.6728443284535811No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAGATCGGAAGAGCACACGT1553980.6600967364252905No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTAGATCGGAAGAGCACACG1205910.5122442086916319No Hit
ATTGACGGGGGCCCGCACAAGCGGTGGAAGATCGGAAGAGCACACGTCTG1131170.48049629039125086Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TTACTGCGATGGAGGGACGGAGAAGGAGATCGGAAGAGCACACGTCTGAA1085100.46092676140946653Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAACTGAAGAGTTTGATCATGAGATCGGAAGAGCACACGTCTGAACTCCA951660.40424436619936677Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAACTGAAGAGTTTGATCATGGCTCAGAAGATCGGAAGAGCACACGTCTG843610.3583470880035389Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
ATTGACGGGGGCCCGCACAAGCGGTGGAGCAGATCGGAAGAGCACACGTC840060.35683912560099207No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGTAGATCGGAAGAGCACACGTCTG802540.34090144972956715Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GTTACTGCGATGGAGGGACGGAGAAGGAGATCGGAAGAGCACACGTCTGA719460.3056108817285548Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGATAGATCGGAAGAGCACAC648310.2753879169563831No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCATAGATCGGAAGAGCACACGTC632230.26855748444005806No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCTAGATCGGAAGAGCACACGTCT576130.24472743069840194Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAAGATCGGAAGAGCACA557620.2368647872980801No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATCGGAAGAGCACACGTCTGAAC544460.23127470695511765Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
AACACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCACACGT535050.22727754464301453No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGAAGATCGGAAGAGCACACG490430.20832394396649587No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAAGATCGGAAGAGCACACG488070.2073214675524084No Hit
CGCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGATCGGAAGAGCACACGT473530.20114519337408968No Hit
TCCGCGATAGCTCAGTCGGTAGAGCAAATGACTAGATCGGAAGAGCACAC440310.1870340635113835No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTAGATCGGAAGAGCACACGTC400430.17009391122587106No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACAGATCGGAAGAGCACA390670.16594807656422106No Hit
ATTGACGGGGGCCCGCACAAGCGGTGGAGATCGGAAGAGCACACGTCTGA378200.16065109313893675Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TTACTGCGATGGAGGGACGGAGATCGGAAGAGCACACGTCTGAACTCCAG367380.15605499364723052Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAAAGATCGGAAGAGCACAC359090.1525335828536774No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGAGATCGGAAGAGCACACGT351000.14909712768843678No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAAGATCGGAAGAGCACACGTCT328460.13952262837761809Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACTGAAGAGTTTGATCATGGCTCAAGATCGGAAGAGCACACGTCTGAA305330.1296975099632775Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCGGATTAGCTCAGTCGGTAGAGCAGCTAGATCGGAAGAGCACACGTCT293520.1246808801114244Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
ATTGACGGGGGCCCGCACAAGCGGTGGAGAGATCGGAAGAGCACACGTCT291820.12395875727076815Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TCAAGGCCGAGAGCTGATGACGAGTCAGATCGGAAGAGCACACGTCTGAA291400.1237803504513119Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCAGATCGGAAGAGCACACGT281580.11960902910116816No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTG273310.11609611387044631Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AACACATGCAAGTCGAGCGGATGAAGGGAGCTAGATCGGAAGAGCACACG271610.11537399102979008No Hit
GAACTGAAGAGTTTGATCATGGCTAGATCGGAAGAGCACACGTCTGAACT270680.11497894735813695Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
ATTGACGGGGGCCCGCACAAGCGGTGGAGTAGATCGGAAGAGCACACGTC270570.11493222176256507No Hit
GCGGACGTGGTGGAATTGGTAGACACACTGGATAGATCGGAAGAGCACAC264020.11214992493533071No Hit
TTACTGCGATGGAGGGACGGAAGATCGGAAGAGCACACGTCTGAACTCCA262850.11165293450970258Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GAGGATGATCAGTCACACTGGAACTGAAGATCGGAAGAGCACACGTCTGA254490.10810178924624012Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)