FastQCFastQC Report
Fri 23 Jun 2023
ERR4777064.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4777064.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4266280
Total Bases426.6 Mbp
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAAGATCGGAAGAGCACACGTCT3846949.01708279812858Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG3030997.104526660228583Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACAGATCGGAAGAGCACACGTC2016464.7265064646483586No Hit
AGGCACGTAGCTCAGTTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT1203272.8204196630319625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAGATCGGAAGAGCACACGT1180412.766836682074313No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT492291.1539092605267354No Hit
GAACTGAAGAGTTTGATCATGGCTCAGAAGATCGGAAGAGCACACGTCTG411970.9656421988242684Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TCAAGGCCGAGAGCTGATGACGAGTCAGATCGGAAGAGCACACGTCTGAA269090.6307368480268525Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GAACTGAAGAGTTTGATCATGGCTCAGATCGGAAGAGCACACGTCTGAAC243800.5714580383847286Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GCTCATGTAGCTCAGTTGGTAGAGCACACCCTAGATCGGAAGAGCACACG241800.5667701135415396No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTAGATCGGAAGAGCACACGTC226300.5304386960068256No Hit
GCACTCATAGCTCAGCTGGATAGAGTACTCGAGATCGGAAGAGCACACGT226090.5299464638982908No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGAGATCGGAAGAGCACACGTCTG224280.5257038919152048Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGTAGATCGGAAGAGCACACGTCTG197900.4638701632335431Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAAGATCGGAAGAGCACACG171670.40238802891512043No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACAGATCGGAAGAGCACA168630.39526238315347334No Hit
GAACTGAAGAGTTTGATCATGGCTAGATCGGAAGAGCACACGTCTGAACT148000.3469064383959796Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
AACACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCACACGT147950.34678924027489993No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAAGATCGGAAGAGCACA144630.33900728503520633No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAAAGATCGGAAGAGCACAC141690.3321160355157186No Hit
GAACTGAAGAGTTTGATCATGGCTCAAGATCGGAAGAGCACACGTCTGAA140240.32871729000440664Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGAGATCGGAAGAGCACACGTC140220.32867041075597475No Hit
GGAGAGATGCCGGAGTGGTCGAACGGGACGGATTAGATCGGAAGAGCACA134110.31434880036003265No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAAGATCGGAAGAGCACACG126940.2975425897972004No Hit
GAACTGAAGAGTTTGATCATGAGATCGGAAGAGCACACGTCTGAACTCCA123230.288846489213085Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTAGATCGGAAGAGCACACGTCT121730.28533054558069326Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCATGTAGCTCAGTTGGTAGAGCACACCCTTAGATCGGAAGAGCACAC119380.27982223388994626No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTGATCAGATCGGAAGAGCA109900.2576014701332308No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCAGATCGGAAGAGCACACGT109810.25739051351528736No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATG97840.22933328332880165TruSeq Adapter, Index 7 (100% over 49bp)
GTCCCCTTCGTCTAGTGGCCTAGGACACCAGATCGGAAGAGCACACGTCT97690.22898168896556248Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTG96190.22546574533317082Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGAGATCGGAAGAGCACA94330.22110597522900516No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAGATCGGAAGAGCACAC94180.22075438086576596No Hit
GGGGCTATAGCTCAGCTGGGAGAGCAGATCGGAAGAGCACACGTCTGAAC92690.21726187685759019Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATAGATCGGAAGAGCACAC88250.2068546837057108No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGATAGATCGGAAGAGCACAC88010.20629213272452818No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCAGATCGGAAGAGCA87980.20622181385188035No Hit
GCCGAGGTGGTGGAATTGGTAGACACGCAGATCGGAAGAGCACACGTCTG84460.19797106612786786Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
AGGCACGTAGCTCAGTTGGTTAGAGCACTAGATCGGAAGAGCACACGTCT82340.1930018657940876Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGGGAATAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTGA81900.19197052232858602Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTAGATCGGAAGAGCACACGTCTG81470.1909626184873004Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACTGAAGAGTTTGATCATGGCTCAGATAGATCGGAAGAGCACACGTCT81110.1901187920155264Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCATGTAGCTCAGTTGGTAGAGCACACAGATCGGAAGAGCACACGTCT80510.18871241456256974Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACAGATCGGAAGAGCAC79200.185641823790281No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAAGATCGGAAGAGCACACGTCT74340.17425016642133193Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AACACATGCAAGTCGAGCGGATGAAGGAGATCGGAAGAGCACACGTCTGA72580.1701247925593257Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCAGGTGGCGGAATTGGTAGACGCACAGATCGGAAGAGCACACGTCTG71780.16824962262205012Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
CGCGGGGTGGAGCAGTCTGGTAGCTCGTCGAGATCGGAAGAGCACACGTC70110.16433520537798738No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTAGATCGGAAGAGCACACG68490.16053798625500437No Hit
GGGGCTATAGCTCAGCTGGGAGAAGATCGGAAGAGCACACGTCTGAACTC66570.156037578405543Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGATAGATCGGAAGAGCAC66510.15589694066024734No Hit
GCCCAGGTGGCGGAATTGGTAGACGCACTAGATCGGAAGAGCACACGTCT64540.15127933468970625Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTAGATCGGAAGAGC63100.14790402880261025No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGAAGATCGGAAGAGCACACGTCTG62400.14626325510749413Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCATAGATCGGAAGAGCACACGTC62060.145466307884152No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCAGATCGGAAGAGCACACGTCTGAA61660.14452872291551422Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCTTGACGATCATAGAGCGTTGGAACCAGATCGGAAGAGCACACGTCTG61060.14312234546255753Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCCAGGTGGCGGAATTGGTAGACGCACTAAGATCGGAAGAGCACACGTC59790.1401455131871326No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGGAGATCGGAAGAGCAC59110.13855161874044836No Hit
GTCCCCTTCGTCTAGTGGCCTAGGACACCGAGATCGGAAGAGCACACGTC58640.13744995640229896No Hit
GCACTCATAGCTCAGCTGGATAGAGTACTCGGAGATCGGAAGAGCACACG57760.13538726947129584No Hit
GAACTGAAGAGTTTGATCATGGCAGATCGGAAGAGCACACGTCTGAACTC57500.13477783924168127Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GCCTCGGTGGCGGAATCGGTAGACGCGGCAGATCGGAAGAGCACACGTCT56060.13140253335458527Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCAGATCGGAAGAGCACACGTCTGA55340.12971488041103726Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GAACTGAAGAGTTTGATCATGGCTCAGAGATCGGAAGAGCACACGTCTGA54370.12744123686209063Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GGGTGATTAGCTCAGCTGGGAGAGCATCTGCAGATCGGAAGAGCACACGT54240.12713652174728335No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGAGATCGGAAGAGCAC51280.12019839297936374No Hit
GCCTCGGTGGCGGAATCGGTAGACGCGAGATCGGAAGAGCACACGTCTGA50660.1187451362779752Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCTCATGTAGCTCAGTTGGTAGAGCACACCCAGATCGGAAGAGCACACGT50530.11844042116316791No Hit
GGGTCGTTAGCTCAGTCGGTAGAGCAGTTGGAGATCGGAAGAGCACACGT50420.11818258529679253No Hit
GGCCTAACACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCA49950.11708092295864313No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCAAGATCGGAAGAGCACACGTCTGA49490.11600270024470967Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCTCGGTGGCGGAATCGGTAGACGCGGCGGATTAGATCGGAAGAGCACA49270.11548702851195888No Hit
GGGGCTACAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT49100.11508855490028784No Hit
ATCTGCCGTGGACGTTTGAGATTTGAGAGGGGCAGATCGGAAGAGCACAC48460.11358841895046738No Hit
AACACATGCAAGTCGAGCGGATGAAGGGAGCTAGATCGGAAGAGCACACG47500.11133821502573671No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGAGATCGGAAGAGCACACGT47210.11065846592347431No Hit
GCTCATGTAGCTCAGTTGGTAGAGCACACCAGATCGGAAGAGCACACGTC45900.10758787515118558No Hit
AGGCACGTAGCTCAGTTGGTTAGAGCAGATCGGAAGAGCACACGTCTGAA45830.10742379778167398Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCACTCATAGCTCAGCTGGATAGAGTACTCAGATCGGAAGAGCACACGTC45380.10636901469195646No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGAGATCGGAAGAG44290.1038140956524185No Hit
TCCGCGATAGCTCAGTCGGTAGAGCAAATGACAGATCGGAAGAGCACACG43330.10156389172768783No Hit
TGCTTGACGATCATAGAGCGTTGAGATCGGAAGAGCACACGTCTGAACTC43190.1012357369886646Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)