FastQCFastQC Report
Sat 24 Jun 2023
ERR4777065.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4777065.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13601388
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAGATCGGAAGAGCACACGTCTG4291043.154854489850595Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGCAAGATCGGAAGAGCACACGTCT3783592.7817675666630493Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAGATCGGAAGAGCACACGT2728112.005758529938268No Hit
GGGTCTGTAGCTCAGTTGGTTAGAGCGCACAGATCGGAAGAGCACACGTC2285901.6806372996638286No Hit
AACACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCACACGT1540721.1327667440999403No Hit
GAACTGAAGAGTTTGATCATGGCTCAGAAGATCGGAAGAGCACACGTCTG1091950.8028224766472364Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATGAGATCGGAAGAGCACA970920.7138389111464212No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCAGATCGGAAGAGCACACGT791750.5821097082150734No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACAGATCGGAAGAGCACA786100.5779557204014767No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCATAGATCGGAAGAGCACAC767740.5644570980549927No Hit
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATG732150.5382906509247438TruSeq Adapter, Index 8 (100% over 49bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAAGATCGGAAGAGCACACG654090.4808994493797251No Hit
AGGCACGTAGCTCAGTTGGTTAGAGCACCAGATCGGAAGAGCACACGTCT646650.47542941940925443Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAGATCGGAAGAGCACAC580960.4271328779092251No Hit
GGAGAGGTGGCCGAGTGGCCGAAGGCGCTAGATCGGAAGAGCACACGTCT578620.42541246525722226Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GAACTGAAGAGTTTGATCATGGCTCAGATCGGAAGAGCACACGTCTGAAC575090.4228171418975769Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAAAGATCGGAAGAGCACAC572940.42123642087116403No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTAGATCGGAAGAGCACACGTC533860.39250405914455205No Hit
TCCGCGATAGCTCAGTCGGTAGAGCAAATGACTGAGATCGGAAGAGCACA458910.33739938894471655No Hit
GAACTGAAGAGTTTGATCATGAGATCGGAAGAGCACACGTCTGAACTCCA453150.3331645270320941Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGATCAGATCGGAAGAGCA449580.33053979490916663No Hit
GCGGGAATAGCTCAGTTGGTAGAGCACGAGATCGGAAGAGCACACGTCTG439940.32345228295818046Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGATAGATCGGAAGAGCACAC433380.318629245779916No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTGAAGATCGGAAGAGCACA384600.2827652589573946No Hit
TGCTTGACGATCATAGAGCGTTGGAACCACCTAGATCGGAAGAGCACACG359830.264553882294954No Hit
GCTCATGTAGCTCAGTTGGTAGAGCACACCCTTAGATCGGAAGAGCACAC322830.23735077625901121No Hit
GCACTCATAGCTCAGCTGGATAGAGTACTCGGCAGATCGGAAGAGCACAC314970.2315719542740785No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAAGATCGGAAGAGCACACGTCT277540.2040527040328531Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAGATCGGAAGAGCACACGT274980.2021705431827987No Hit
TGCTTGACGATCATAGAGCGTTGGAACCAGATCGGAAGAGCACACGTCTG261540.192289198720013Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACTGAAGAGTTTGATCATGGCTCAAGATCGGAAGAGCACACGTCTGAA260140.1912598920051395Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GCCCAGGTGGCGGAATTGGTAGACGCACTAGATCGGAAGAGCACACGTCT248430.1826504765543046Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCGTGTGTGAAGAAGGTCTTCGGATTGTAAAGCACAGATCGGAAGAGCAC247990.18232698015820148No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTAGATCGGAAGAGCACACGTCT242300.17814358358132273Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGAGATCGGAAGAGCACACGT242220.17808476605475854No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATAGATCGGAAGAGCACACGTCT241730.17772450870455278Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GGGTCTGTAGCTCAGTTGGTTAGAGCGTAGATCGGAAGAGCACACGTCTG238350.17523946820721534Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCCGAGGTGGTGGAATTGGTAGACACGCTAGATCGGAAGAGCACACGTCT237880.17489391523865067Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
GCTCATGTAGCTCAGTTGGTAGAGCACACCCTAGATCGGAAGAGCACACG226140.16626244321535422No Hit
GGAGAGATGCCGGAGTGGTCGAACGGGACGGATTAGATCGGAAGAGCACA222180.16335097565042628No Hit
GGAGAGGTGGCCGAGTGGCCGAAGGCGCAGATCGGAAGAGCACACGTCTG220790.16232902112637326Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TGCTTGACGATCATAGAGCGTTGGAACCACCTGATAGATCGGAAGAGCAC206200.15160217471922718No Hit
ACACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCACACGTC205470.15106546478932883No Hit
ATTGACGGGGGCCCGCACAAGCGGTGGAAGATCGGAAGAGCACACGTCTG199930.14699235107475797Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCAGATCGGAAGAGCA197890.14549250414737086No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGGAAGATCGGAAGAGCACACG197420.14514695117880616No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCTTGCAAGATCGGAAGAGCACACG190230.13986072597884863No Hit
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGATCGGAAGAGCACACGTCTG187590.13791974760223003Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GGTGGGCGTAGCTCAGTTGGTAGAGCACAGAGATCGGAAGAGCACACGTC180100.1324129566776567No Hit
AACACATGCAAGTCGAGCGGATGAAGGGAGATCGGAAGAGCACACGTCTG176250.12958236321175456Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GAACTGAAGAGTTTGATCATGGCTAGATCGGAAGAGCACACGTCTGAACT175510.1290383010910357Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CGGGGCGTAGCGCAGCCTGGTAGCGCACTTGCATGAGATCGGAAGAGCAC167640.12325212691528246No Hit
ACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCACACGTCTG163310.12006862828999512Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
TAACACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCACACG163150.11995099323686671No Hit
TCCAAGTTTAAGGTGGTAGGCTGAGATCGGAAGAGCACACGTCTGAACTC156850.11531911301993591Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
GGCCTAACACATGCAAGTCGAGCGGATGAAGGGAGCAGATCGGAAGAGCA148760.10937119064613113No Hit
GCGGACGTGGTGGAATTGGTAGACACACTGGATTAGATCGGAAGAGCACA147880.10872419785392491No Hit
GAACTGAAGAGTTTGATCATGGCTCAGATTGAACGAGATCGGAAGAGCAC147060.10812131820664185No Hit
GCACTCATAGCTCAGCTGGATAGAGTACTCGGAGATCGGAAGAGCACACG146870.10798162658105187No Hit
GAACTGAAGAGTTTGATCATGGCTCAGAGATCGGAAGAGCACACGTCTGA141330.10390851286648098Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
GCCCAGGTGGCGGAATTGGTAGACGCACAGATCGGAAGAGCACACGTCTG140480.10328357664673635Illumina Multiplexing PCR Primer 2.01 (100% over 22bp)
GCGGACGTGGTGGAATTGGTAGACACACTGGATAGATCGGAAGAGCACAC139200.10234249622170914No Hit
GGGGCTATAGCTCAGCTGGGAGAGCGCAGATCGGAAGAGCACACGTCTGA138110.1015411074222719Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)