FastQCFastQC Report
Sat 30 Sept 2023
ERR4882133_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR4882133_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41788367
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2396900.5735806809584112No Hit
GGGCAACCAGCTATCACCAAGCTCGATAGGCTTTTCACCTCTACCTAAAA1106160.2647052467975119No Hit
GTGGAATTAGTGTGTGTAAGTATGTATGTTGAGCTTGAACGCTTTCTTTA1103420.26404956192712675No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1088400.260455260192388No Hit
GGCAGATGTAAAGTAGGCTCGGGTGTCTACATCTAGGCCTACTGTGAATA931960.22301900430806496No Hit
GGGGGTTCGAATCCTTCCTTTCTTATTTAACTTTTACGTAGGAAGGTTCT848260.20298950662513324No Hit
CGAAGAATCAGAATAGGTGTTGATAGAGAATTGGGTCTCCACCTCCAGCG740690.17724789293632842No Hit
GCTCGTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTT727580.17411065620247856No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT595880.1425947082354283No Hit
GTACACTTCTGGGTGGCCGAAGAATCAGAATAGGTGTTGATAGAGAATTG595400.14247984373258712No Hit
GTCTGATATTGGGTTATAGCAGGGGGTTTTATATTAATGATAGTGGTGAT590280.14125462236894779No Hit
GGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTAGTTAATTCAT589890.14116129496038934No Hit
GTTGGGTTAGTACCTATGATTCGATAATTGACAATGGTTATCCGGGTTGT589730.14112300679277562No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA562940.13471213172795193No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT562230.134542227984166No Hit
GTTTGGTTTCGGGGTTCTTAGCTTAAATTCTTTTTGTTAAGGATTTTCTA558090.1335515216471608No Hit
ATGAAATTGGCTTGAAACCAGTTGTAGGGGGTTCGAATCCTTCCTTTCTT555830.1330107012796169No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA547910.13111543698273734No Hit
GGAGAATTGGTTCTTGTTACTCATATTAACAGTATTTCATCTATGGGTCT524920.12561390589873972No Hit
GTGACGATGACATTATAGATTTGGTCATCTCCTAGGAGTGCGCCTGGCTG487680.11670233488664442No Hit
GTCGAAGAAAGTAGTATTTAGATTTCGGTCTGTAAGGAGTATAGTGATAC478760.11456776954217905No Hit
GTAAGCATCTGGATAATCAGAGTAACGACGAGGTATCCCCGCTAATCCTA470930.11269404233958222No Hit
GCACGTTTTACGCCGAAAATAATTAGTTTGGGTTAATCGTATGACCGCGG469130.11226330045392777No Hit
GGCGAATACTGCTCCTATAGATAAGACATAGTGGAAGTGAGCTACTACAT465140.11130848927406042No Hit
GTGGAAGTGAGCTACTACATAGTATGTATCATGAAGTACAATGTCAAGGG458420.10970038623428383No Hit
GGGGTATACTGTTCATCCTGTTCCAGCTCCAGCTTCTACTATGGAGGATG454080.10866181968776144No Hit
GTGGGCTTTTGCTCATGTGTCATTTAGGGTATAGCCTGAGAATAGTGGGA447750.10714704405654331No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG423580.10136313773639444No Hit
GCCGTAAGTGAGATGAATGAGCCTATAGAGGAGACTGTATTTCATGTGGT420960.10073616899171964No Hit
GGTTGATTCATGAAATTGTTTTTAGGTAGCTCGTTTGGTTTCGGGGTTCT420460.10061651846792674No Hit
GTTGTAGTTGTTTATTCACTATTTAAGGTTTTTTCCTTTTCCTAAAGAGC419670.10042747064033396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)