FastQCFastQC Report
Mon 26 Jun 2023
ERR5211760.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211760.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111339537
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA19503541.7517173616412651No Hit
ACGGAGACGAAGTGCATGAT16198351.4548605496715872No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA13949961.2529206044749406No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT9739650.8747701187225163No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA7760700.6970300226773891No Hit
ACGGAGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG7492810.6729693873255463No Hit
TGTGCCTGAGATGTGATGGGACTCTC6570460.5901281949825244No Hit
AGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG5910400.5308446720054171No Hit
TATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT5338640.47949184484214263No Hit
ACGGAGACGAAGTGC5262720.4726730631186296No Hit
ATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA4713760.4233680260409202No Hit
AGTTCTGACGGTACCTGATG4056230.36431173591102683No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGT3861750.3468444457425757No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG3623910.32548276179736585No Hit
AGGAGGGTAAGTCGAACTAGT3598560.3232059425574942No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT3581250.3216512387688481No Hit
AGGAGGGTAAGTCGAACTAG3534980.31749548231011593No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT3523360.31645182788931486No Hit
AGAAGTTCTGACGGTACCTGATG3467970.3114769553963566No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCT3184110.2859819688310721No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAG2901000.2605543437817601No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG2863420.25717908275476303No Hit
AGGAGGGTAAGTCGAACT2598770.23340944915192166No Hit
ATATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT2591680.23277265828759464No Hit
AATAGACCGAGGGCTTGACC2584590.2321358674232676No Hit
ACTAATAGACCGAGGGCTTGACC2473620.2221690575199715No Hit
AGTTTTTCACTGCCTTTCGGAGGGTAGAAGTAATGAATAATCGTAAACGT2213930.1988449080760952No Hit
ATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT2181200.19590525151905383No Hit
ATGGATGAAGTCGAGGAGTACTAATAGACCGAGGGCTTGAC2178520.19566454636864533No Hit
AGACCGAGGGCTTGACC2137250.19195786668306336No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG2135470.19179799535182188No Hit
AATGGATGAAGTCGAGGAGTACTAATAGACCGAGGGCTTGAC1925110.17290443735184566No Hit
TATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCC1806790.16227748459201874No Hit
AGGAGGGTAAGTCGAACTAGTTACT1722880.15474107818501168No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTC1707750.1533821718694591No Hit
AAGGAGGGTAAGTCGAACTAGT1694490.1521912202670647No Hit
GTGCCTGAGATGTGATGGGACTCTC1641410.14742382124330192No Hit
AAGTTCTGACGGTACCTGATG1641320.1474157378613852No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGGG1626390.14607479461675865No Hit
ACTAATAGACCGAGGGCTTGAC1602840.1439596430152211No Hit
AAGGAGGGTAAGTCGAACTAG1558070.139938609588434No Hit
AGTACTAATAGACCGAGGGCTTGACC1522360.13673130327459507No Hit
AGGAGGGTAAGTCGAACTAGTT1470190.13204563622354565No Hit
AAGATTGGGGTCGTGGGACAGC1452050.13041638569055664No Hit
ACGGAGACGAAGTGCATGAC1372600.12328055576519956No Hit
TCGAAGATTGGGGTCGTGGGACAGC1345780.12087170795402175No Hit
TATCCGGTGTAGGCGTAAGAGT1279210.11489269979629968No Hit
CGCGGGATAGAGCAGTCTGGT1267070.11380234139109094No Hit
AGACCGAGGGCTTGAC1261050.11326165295621807No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACTG1245150.11183358881760035No Hit
AGTACTAATAGACCGAGGGCTTGAC1229110.11039295052933443No Hit
TGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1227910.11028517210377838No Hit
TGCCTGAGATGTGATGGGACTCTC1220890.10965466831427545No Hit
GTACTAATAGACCGAGGGCTTGACC1199530.1077362123393777No Hit
TAATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA1171500.10521868794909754No Hit
AGGCGGGAGAGAGCAAACCTAGTGAACTG1153020.10355890019553432No Hit
ACTAAGTGGAGGTCCGAACCGAC1148480.1031511384855139No Hit
AAGGAGGGTAAGTCGAACT1141150.10249279193607569No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)