FastQCFastQC Report
Mon 26 Jun 2023
ERR5211761.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211761.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113014980
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT13638671.2068019655447446No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA6736350.5960581508752202No Hit
TGTGCCTGAGATGTGATGGGACTCTC6590780.5831775575237902No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT5050830.4469168600481104No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA3864220.3419210444491518No Hit
TAGGCGTGAACTCCGGGCTTCC3184400.281767956778827No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT3156590.27930722104273253No Hit
AGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG2964640.2623227469491213No Hit
AGAAGTTCTGACGGTACCTGATG2551080.22572936791211218No Hit
AGTTCTGACGGTACCTGATG2040100.18051589267192722No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT1834640.16233600182913804No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA1781640.15764635803147514No Hit
GAGGTTGTAGCTCAGACGGAT1721470.1523222850634491No Hit
AGACCGAGGGCTTGACC1654190.14636909195577436No Hit
CGCGGGATAGAGCAGTCTGGT1642270.14531436452052637No Hit
ACGGAGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG1613430.14276249042383585No Hit
GTGCCTGAGATGTGATGGGACTCTC1572820.1391691614686832No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAG1404060.12423662774616251No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1399630.12384464431175407No Hit
TATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT1345040.11901431120016125No Hit
ACGGAGACGAAGTGCATGAC1324540.11720039237276332No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG1269220.1123054660541461No Hit
ACGGAGACGAAGTGC1259690.11146221500901916No Hit
TTCTTGGTGTCTTTAGCGTCATGGAACCACTCCGATC1202780.10642659937647204No Hit
AGGCGTGAACTCCGGGCTTCC1160740.10270673852262771No Hit
TGCCTGAGATGTGATGGGACTCTC1150470.10179800943202397No Hit
ACTAATAGACCGAGGGCTTGACC1149860.10174403428642823No Hit
AAGTTCTGACGGTACCTGATG1136390.10055215689105992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)