FastQCFastQC Report
Thu 29 Jun 2023
ERR5211762.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211762.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61141740
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT13658862.2339665177994608No Hit
TCTTTTCGAAGATTGGGGTCGTGGGACAG10517051.7201096992005789No Hit
TGTGCCTGAGATGTGATGGGACTCTC7093151.160115822676947No Hit
CTTTTCGAAGATTGGGGTCGTGGGACAG3767060.6161192010564305No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACC3742580.6121153895849218No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT2909970.47593836878047635No Hit
ACGGAGACGAAGTGC2241760.3666496897209664No Hit
TGCCTGAGATGTGATGGGACTCTC2168810.3547183969576267No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT1921600.31428611616221586No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA1835100.3001386614119912No Hit
GTGCCTGAGATGTGATGGGACTCTC1728730.2827413809289693No Hit
TCTTTTCGAAGATTGGGGTCGTGGGACAGC1713200.2802013812495359No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA1564600.2558971988693812No Hit
TAGGCGTGAACTCCGGGCTTCC1548660.2532901418899757No Hit
GCCCCCATCGTCTAGAGGCCTAGGACACCT1494830.24448600906680118No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACCGC1397710.22860160669290736No Hit
CGGCGAAAACGTCTTGAGCAGAGGTGTGATGGGGAGTCTCT1259110.20593296821451273No Hit
AATAGACCGAGGGCTTGACC1216870.19902443077347817No Hit
GGGTCTGTAGTTC1215640.19882325887356167No Hit
CGGCGAAAACGTCTTGAGC1211390.19812815271531364No Hit
ACGGAGACGAAGTGCATGAC1162540.19013852075521567No Hit
AGACCGAGGGCTTGACC1024670.16758927698164952No Hit
ACTAATAGACCGAGGGCTTGACC1014290.16589158241162258No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT1010860.16533059085331886No Hit
GAGGTTGTAGCTCAGACGGAT939850.15371659360692058No Hit
TCTTTTCGAAGATTGGGGTCGTGGGACAA908900.14865458523097314No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG896470.14662160416108536No Hit
GGGTCTGTAGTTCAATTGGTTAGAGCACT854300.13972451552736315No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCT838960.1372155911820632No Hit
CTACGGAGACGAAGTGCATGAT833370.1363013221409793No Hit
TGGGGTGTAGCCAAGCGGTAAGGCAGC821280.13432394956375138No Hit
GAAGATTGGGGTCGTGGGACAG808210.13218629368415097No Hit
TCTTTTCGAAGATTGGGGTCGTGGGAC802340.1312262294138178No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTA800200.13087622301884114No Hit
AGTACTAATAGACCGAGGGCTTGACC784630.1283296811638007No Hit
CTTTTCGAAGATTGGGGTCGTGGGACAGC779170.1274366741934397No Hit
AGTTCTGACGGTACCTGATG731920.11970872925762335No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGGG653810.1069334958409754No Hit
TGGGGTGTAGCCAAGCGGTAAGGCAGCG649720.10626455838515556No Hit
ACGGAGACGAAGTGT647050.1058278681633856No Hit
GGGCTTGTAGCTTAGTGGATTAGAGCGCGTG627570.10264182864275696No Hit
CGGCGAAAACGTCTTGAGCAGAGGTGTGATGGGGAGTCT623210.10192873150158957No Hit
CAACGTGGACTGAGTAGATTAGACGAAGCAGCT616380.10081165501668746No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG615520.1006709982411361No Hit
GTACTAATAGACCGAGGGCTTGACC612290.10014271756086758No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)