FastQCFastQC Report
Sun 2 Jul 2023
ERR5211763.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211763.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125744450
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA18546371.4749255334927307No Hit
ACGGAGACGAAGTGCATGAT13430561.0680837205936327No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT9746000.7750640286708479No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA6620880.5265345707106756No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT6499760.5169023364450677No Hit
TGTGCCTGAGATGTGATGGGACTCTC5224480.4154839438241608No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAG4890620.38893326902300657No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT3845260.3057995800212256No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG2744520.21826172049740564No Hit
ACGGAGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG2668010.21217715771948584No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA2438670.19393857939654593No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG2382230.18945011091940836No Hit
GCGGATGTGGCGGAATTGGTATACGCGCACGTTTGAGGGGCGTGTGGC2151370.17109065250991196No Hit
TAGGCGTGAACTCCGGGCTTCC2113210.16805592612636186No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1965350.15629715665383243No Hit
TATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT1523480.1211568383336203No Hit
CGCGGGATAGAGCAGTCTGGT1485610.11814517459816318No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCT1472000.11706282066524605No Hit
GAGGTTGTAGCTCAGACGGAT1397270.11111981483079372No Hit
AGTTCTGACGGTACCTGATG1358290.10801987682160129No Hit
AGAAGTTCTGACGGTACCTGATG1296480.10310435172287923No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGG1291650.10272023934257138No Hit
TGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1287720.10240770069772462No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)