FastQCFastQC Report
Mon 26 Jun 2023
ERR5211764.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211764.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences72010500
Total Bases2.1 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT30609084.25064122593233No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA9189201.2760916810742877No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT7130390.9901875420945557No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT6781260.9417043347845105No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA6430500.892994771595809No Hit
ACGGAGACGAAGTGC5646940.7841828622214817No Hit
TGTGCCTGAGATGTGATGGGACTCTC5392050.7487866352823547No Hit
TAGGCGTGAACTCCGGGCTTCC5100160.7082522687663605No Hit
TGCCTGAGATGTGATGGGACTCTC4536210.6299373008102985No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT2513590.3490588178112914No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA2375230.3298449531665521No Hit
ACGGAGACGAAGTGCATGAC2160210.29998541879309265No Hit
GTGCCTGAGATGTGATGGGACTCTC1923650.2671346539740732No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1776360.24668069239902513No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG1715350.23820831684268268No Hit
AATAGACCGAGGGCTTGACC1704110.23664743336041272No Hit
AGGCGTGAACTCCGGGCTTCC1402290.1947341012768971No Hit
TAGGCGTGAACTCCGGGCTTC1398440.19419945702362848No Hit
ACTAATAGACCGAGGGCTTGACC1270210.17639233167385313No Hit
AGTTCTGACGGTACCTGATG1236400.17169718304969414No Hit
GAGGTTGTAGCTCAGACGGAT1204250.16723255636330814No Hit
TGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1166850.1620388693315558No Hit
AGACCGAGGGCTTGACC1125270.15626471139625472No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCT1122230.15584255073912834No Hit
ACGGAGACGAAGTGT1088200.15111685101478256No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1036740.1439706709438207No Hit
GAGGTTGTAGCTC906750.12591913679255107No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGGG883250.12265572381805431No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAG853040.11846050228786079No Hit
TACGGAGACGAAGTGCATGAT844340.11725234514411093No Hit
AGTACTAATAGACCGAGGGCTTGACC840650.11673991987279633No Hit
TAGGCGTGAACTCCGGGCTTCT794790.11037140417022517No Hit
AGCGGCGACGATACTCTGGGGG783930.10886329077009604No Hit
ACGGAGACGAAGTGCATGAG756700.10508189777879615No Hit
ATATCCGGTGTAGGCGT724330.10058671999222336No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)