FastQCFastQC Report
Mon 26 Jun 2023
ERR5211765.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211765.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108201536
Total Bases3.7 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT19868081.836210532168416No Hit
TGTGCCTGAGATGTGATGGGACTCTC5095770.47095172475185565No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA4796770.4433181059462964No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT4628790.4277933725450995No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA4443220.41064296906099373No Hit
AGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG4144140.38300195664505166No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA3404180.31461475740972844No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT3321630.3069854756960197No Hit
TAGGCGTGAACTCCGGGCTTCC2899860.2680054375568199No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG2493950.23049118267600197No Hit
CGCGGGATAGAGCAGTCTGGT2485060.22966956772221792No Hit
AGTTCTGACGGTACCTGATG2298030.21238423084862676No Hit
ACGGAGACGAAGTGCATGAC1728760.1597722235662163No Hit
GAGGTTGTAGCTCAGACGGAT1711580.15818444573651894No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1696720.1568110826079216No Hit
TATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT1668550.1542076075518928No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT1564700.14460977707377462No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGT1480930.1368677428017288No Hit
ACGGAGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG1476500.13645832162678356No Hit
AGACCGAGGGCTTGACC1461110.1350359758294004No Hit
TGCCTGAGATGTGATGGGACTCTC1402570.129625701431817No Hit
TGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1256630.11613790769107013No Hit
AGAAGTTCTGACGGTACCTGATG1256180.1160963186326671No Hit
TTCTTGGTGTCTTTAGCGTCATGGAACCACTCCGATC1242790.11485881309485292No Hit
ACGGAGACGAAGTGC1219710.11272575649942715No Hit
ATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA1192080.11017218831348198No Hit
AATAGACCGAGGGCTTGACC1170460.10817406510754154No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG1119530.10346710789761802No Hit
GTGCCTGAGATGTGATGGGACTCTC1108990.1024929997296896No Hit
ATGGATGAAGTCGAGGAGTACTAATAGACCGAGGGCTTGAC1094710.10117324027636725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)