FastQCFastQC Report
Mon 26 Jun 2023
ERR5211766.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211766.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences83471777
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT32531863.897348441497777No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA10736241.286211985159966No Hit
ACGGAGACGAAGTGC6377350.7640127273197982No Hit
TGCCTGAGATGTGATGGGACTCTC5676030.6799939097977991No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA4007830.4801419286904603No Hit
TAGGCGTGAACTCCGGGCTTCC3891670.46622584780961357No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA3290170.39416556328973323No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT2928590.35084792791700126No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG2497090.2991538085980846No Hit
TGTGCCTGAGATGTGATGGGACTCTC2469140.2958053714371026No Hit
ATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA2219630.26591383097067645No Hit
ACGGAGACGAAGTGCATGAC2180250.2611960687023591No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGT1970510.23606901288324078No Hit
GTGCCTGAGATGTGATGGGACTCTC1906290.2283753944761473No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT1820680.21811923328288554No Hit
TGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1780460.21330083819828108No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1763390.21125583561016079No Hit
AATAGACCGAGGGCTTGACC1576190.18882909369474665No Hit
ACTAATAGACCGAGGGCTTGACC1250270.1497835609753462No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTC1230590.14742587785090522No Hit
AGACCGAGGGCTTGACC1144010.1370535097150262No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT1117040.13382247750637918No Hit
ACGGAGACGAAGTGT1097980.13153907098443585No Hit
TAATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA1095590.13125274666190465No Hit
ATGGATGAAGTCGAGGAGTACTAATAGACCGAGGGCTTGAC1083530.12980794694235395No Hit
TATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT1050640.1258676929808263No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG1029350.12331713029183505No Hit
AGGCGTGAACTCCGGGCTTCC976010.11692694645760326No Hit
AGTACTAATAGACCGAGGGCTTGACC958870.11487355780145905No Hit
GAGGTTGTAGCTCAGACGGAT946450.11338562973207099No Hit
TAGGCGTGAACTCCGGGCTTC844690.10119468284471769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)