FastQCFastQC Report
Mon 26 Jun 2023
ERR5211767_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211767_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences103060320
Total Bases3.3 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT31205233.0278607712454217No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT21598272.0956921150642653No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA16475501.5986269012166856No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA12874771.2492460725912746No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA9795910.9505025794602616No Hit
TGTGCCTGAGATGTGATGGGACTCTC6813670.6611341785082756No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT4436090.4304362726605157No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG4131130.4008458347499794No Hit
TATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT4097290.39756232078456577No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCT3878460.3763291245359999No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT3696890.3587112867493522No Hit
AGTTCTGACGGTACCTGATG3679540.357027806628196No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAG3089820.2998069480087001No Hit
TAGGCGTGAACTCCGGGCTTCC3031260.2941248387352184No Hit
AACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG2943250.285585179630725No Hit
ATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGTA2622080.2544218764312007No Hit
AATAGACCGAGGGCTTGACC2306960.22384560808660403No Hit
ATGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG2244580.2177928420948043No Hit
AATCCTGAAGCTAGCGACTGAAGCCCCGGTGAACGGCGGCCGT2190060.21250273626163785No Hit
CGCGGGATAGAGCAGTCTGGT2151070.20871951494037666No Hit
ACGGAGACGAAGTGCATGAC2048440.1987612691286035No Hit
AGACCGAGGGCTTGACC1933220.1875814086352536No Hit
TGCCTGAGATGTGATGGGACTCTC1920560.1863530018148595No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGGG1914370.18575238268229713No Hit
ACGGAGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG1904200.18476558194269144No Hit
AGTACTAATAGACCGAGGGCTTGACC1880710.1824863342166995No Hit
ACTAATAGACCGAGGGCTTGACC1860630.1805379606816668No Hit
AGAAGTTCTGACGGTACCTGATG1853170.1798141127448469No Hit
AGACGAAGTGCATGATGTCAAGCTTCCAAGAAAAGCCCGAACCACG1835800.17812869201259998No Hit
ACGGAGACGAAGTGC1783040.1730093599554125No Hit
GTGCCTGAGATGTGATGGGACTCTC1671530.1621894828193819No Hit
ATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT1641150.15924169457265414No Hit
AAGATTGGGGTCGTGGGACAGC1635120.15865660032881715No Hit
GAGGTTGTAGCTCAGACGGAT1627170.1578852074202758No Hit
ATATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCCT1568500.15219242478579537No Hit
CGGGGCGTAGCGCAGCTTGGTAGCGCACCACTTTGG1519990.14748547258537523No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAC1436470.1393814806707373No Hit
ATAGACCGAGGGCTTGACC1431040.1388546047596204No Hit
TCGAAGATTGGGGTCGTGGGACAGC1364470.13239528074432527No Hit
GTACTAATAGACCGAGGGCTTGACC1360060.13196737599883251No Hit
TGAACGGGGCTAAGTGATGTACCGAAGCTGTGGACTCAG1320720.12815019398348462No Hit
TATCCGGTGTAGGCGTAAGAGTATTGAGAGGAGACTTCC1212340.11763402248314385No Hit
ATGGATGAAGTCGAGGAGTACTAATAGACCGAGGGCTTGAC1192990.11575648125292062No Hit
AAGTTCTGACGGTACCTGATG1139170.11053429680792763No Hit
GAGGTTGTAGCTC1108790.10758650856119989No Hit
TTCTTGGTGTCTTTAGCGTCATGGAACCACTCCGATC1063140.10315706374674559No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACTG1061320.10298046813749463No Hit
ACTAAGTGGAGGTCCGAACCGAC1059200.10277476336188361No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)