FastQCFastQC Report
Mon 26 Jun 2023
ERR5211768.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211768.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115682707
Total Bases2.9 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGGAGACGAAGTGCATGAT37602853.250516086211572No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA31929192.7600659448607128No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT21308221.84195378484703No Hit
AGGAGGGTAAGTCGAACT19695661.70255870654894No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA16563291.4317861700798546No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT13054261.1284538837771145No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT8899590.7693103170554264No Hit
TAGGCGTGAACTCCGGGCTTCC8256290.7137013140607092No Hit
AGGAGGGTAAGTCGAACTAG6801310.5879279778610298No Hit
AAGGAGGGTAAGTCGAACT6574760.5683442383484335No Hit
AGTTCTGACGGTACCTGATG5531250.4781397447762007No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCT5326800.46046640315911697No Hit
AGTTCAGATTGCAGGCTG4651370.4020799755316929No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAG4390040.3794897365256157No Hit
ACAGCGGCGACGATACTCTGGGGG4059830.3509452800062848No Hit
AGCTGGTCTGAGAGGATG3921180.33895991040389467No Hit
ACGGAGACGAAGTGCATGAC3727620.3222279367995771No Hit
AGGAAGCTTGACTGTGAGACTG3664940.3168096680171912No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG3645640.315141311484006No Hit
AATAGACCGAGGGCTTGACC3525480.3047542793064135No Hit
TAGCTGGTCTGAGAGGATG3419880.29562586221292353No Hit
AGGCGTGAACTCCGGGCTTCC3387770.2928501664470905No Hit
AGAAGTTCTGACGGTACCTGATG3359880.29043926159162237No Hit
AGTAGCTGGTCTGAGAGGATG3341760.2888729082039894No Hit
AAGTGATGTACCGAAGCTGTGGAC3125180.27015100882796594No Hit
AGACCGAGGGCTTGACC3110630.2688932581773004No Hit
ATAGACCGAGGGCTTGACC3032390.2621299309671237No Hit
AAGCTTGACTGTGAGACTG2897340.25045575740201165No Hit
TGACAGCGGCGACGATACTCTGGGGG2626880.22707629066805982No Hit
AGCGGCGACGATACTCTGGGGG2612930.22587040602360733No Hit
AAGGAGGGTAAGTCGAACTAG2555790.22093103336525483No Hit
AGTACTAATAGACCGAGGGCTTGACC2552820.22067429663450042No Hit
ATCAGTAGCTGGTCTGAGAGGATG2485120.21482208226679894No Hit
ACGGAGACGAAGTGC2482880.21462844917693705No Hit
AGGAGGGTAAGTCGAACC2348190.20298539521555284No Hit
AAGTGATGTACCGAAGCTGTGGACTC2293420.19825089328174175No Hit
TCACATCGACGCGGAGGTTTGGCACC2210530.1910856045234142No Hit
ACTAATAGACCGAGGGCTTGACC2176220.18811973340146682No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAC2156320.1863995108620686No Hit
GTACTAATAGACCGAGGGCTTGACC2085680.18029315306392338No Hit
AAGTTCTGACGGTACCTGATG2069050.17885560025838607No Hit
AGGAGGGTAAGTCGAACTAGTTACT2052540.17742842065409137No Hit
AAAGGAGGGTAAGTCGAACT1947130.1683164277959021No Hit
ACATCGACGCGGAGGTTTGGCACC1881130.16261116711247084No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACTG1865920.16129636385497098No Hit
TAGGCGTGAACTCCGGGCTTC1859960.1607811615265884No Hit
AGGCGTAACGATCTGGGCAC1838430.15892003633697818No Hit
AGGACAATTTCAGGTGGGC1818730.15721710246631762No Hit
AGTGATGTACCGAAGCTGTGGAC1767690.15280503420446412No Hit
GAGGTTGTAGCTC1761630.15228118754171271No Hit
ATGTGACAGCGGCGACGATACTCTGGGGG1626990.14064245574751288No Hit
ACGATGGATACTAGGCGTGGCTTGT1594630.1378451491457578No Hit
TAGGCGTGAACTCCGGGCTTCT1510950.13061157014591646No Hit
TGGGGTGTAGCCAAGCGGTAAGGCAGCGGG1493140.1290720141948269No Hit
GCGGAGACGAAGTGCATGAT1409400.12183324859436424No Hit
AGGAGTACTAATAGACCGAGGGCTTGACC1385200.1197413196771061No Hit
AAACTGACACTGAGGGACG1380780.11935924009800358No Hit
AGGAGGGTAAGTCGAACTAGT1365490.11803752137300869No Hit
ACGATGGATACTAGGCGTGGCTTG1331710.11511746522321611No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAC1326170.11463856909918264No Hit
ACGAGTGACCGTGTGCCTG1287970.11133643337028758No Hit
AGTGATGTACCGAAGCTGTGGACTC1278260.11049706850307366No Hit
TGTGACAGCGGCGACGATACTCTGGGGG1218410.10532343438332575No Hit
TCAGTAGCTGGTCTGAGAGGATG1203780.10405876826516518No Hit
AAGGAAGCTTGACTGTGAGACTG1196210.10340439215344432No Hit
ATCGACGCGGAGGTTTGGCACC1192210.10305861877869094No Hit
ACTATAACGGTCCTAAGGTAGCG1180320.10203080742223641No Hit
AGGACGTGTCAAGGAGAGAAGCTGATG1174710.10154586026414475No Hit
ATAGGCGGGAGAGAGCAAACCTAGTGAACTG1160970.10035812872186678No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)