FastQCFastQC Report
Mon 26 Jun 2023
ERR5211769_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211769_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138479465
Total Bases3.5 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTTCAGATTGCAGGCTG9801020.7077598111748915No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT8303870.5996463085700108No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA8039860.5805813880057957No Hit
TAGCTGGTCTGAGAGGATG6319980.45638391222843044No Hit
AGCTGGTCTGAGAGGATG5959360.4303425060170473No Hit
ACGGAGACGAAGTGCATGAT5644960.40763877878933175No Hit
AGTTCTGACGGTACCTGATG4892190.3532790944852365No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT4305490.3109118019772823No Hit
AGAAGTTCTGACGGTACCTGATG3964370.2862785467867023No Hit
TCACATCGACGCGGAGGTTTGGCACC3913040.2825718600227117No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA3826600.2763297792925471No Hit
AGTAGCTGGTCTGAGAGGATG3628430.26201935427754575No Hit
AGCGGCGACGATACTCTGGGGG3493950.25230816713510557No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACTG3320310.23976912389140154No Hit
ACAGCGGCGACGATACTCTGGGGG3317000.23953009928222932No Hit
ATGTACACGGAGTCGACCCGCAACGCGA2842330.20525281492097042No Hit
AAGTGATGTACCGAAGCTGTGGAC2811000.20299038561421365No Hit
AGGCGGGAGAGAGCAAACCTAGTGAACTG2764310.19961876658030128No Hit
ATAGGCGGGAGAGAGCAAACCTAGTGAACTG2763200.19953861029142478No Hit
CGCGGGATAGAGCAGTCTGGT2473640.17862865082559354No Hit
TCACATCGACGCGGAGGTTTGGCACT2440980.1762701783979307No Hit
TCACATCGACGCGGAGGT2389960.1725858776245272No Hit
AAGTTCTGACGGTACCTGATG2345790.16939623503022633No Hit
TGACAGCGGCGACGATACTCTGGGGG2320810.1675923574661413No Hit
AATGGATGAAGTCGAGGAGT2226790.16080290315968507No Hit
TCTCGGAGAGAGGCTCGGCGAAATAGG2220480.16034723993192782No Hit
ATGAGCTGTGGTTAGGGG2183920.15770713730010438No Hit
AGTTCAGATTGCAGGCTA2001030.1445001249824297No Hit
ACGATGGATACTAGGCGTGGCTTGT1975460.14265364182335627No Hit
AGTGATGTACCGAAGCTGTGGAC1858970.13424156426369788No Hit
ATGGATGAAGTCGAGGAGT1733510.1251817372344701No Hit
ACATCGACGCGGAGGTTTGGCACC1663910.12015572128329642No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACT1620450.1170173498287273No Hit
ATGTGACAGCGGCGACGATACTCTGGGGG1514010.10933101164132891No Hit
TAGGCGTGAACTCCGGGCTTCC1508910.10896272598973429No Hit
AGGCGGGAGAGAGCAAACCTAGTGAACT1411350.10191763811334771No Hit
AGTAGCTGGTCTGAGAGG1399830.10108574581798102No Hit
CACAATGGAGAGTTTGATCCTGGCTCAGG1390930.10044305124951199No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)