FastQCFastQC Report
Thu 29 Jun 2023
ERR5211770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR5211770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148112090
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length6-70
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGGAACTGGCGGAATTGGTAGACGCGCTAGA34205932.309462380822524No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAT28041941.8932917630154298No Hit
AGTTCAGATTGCAGGCTG25809151.7425417465920574No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAT15010421.0134500161330515No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGA10834950.7315371756620274No Hit
AGTTCTGACGGTACCTGATG10593100.7152083263425694No Hit
TAGCTGGTCTGAGAGGATG9875780.6667774386277312No Hit
AGCTGGTCTGAGAGGATG9386700.6337565015793106No Hit
AGAAGTTCTGACGGTACCTGATG8653350.584243325443588No Hit
AGTAGCTGGTCTGAGAGGATG7774490.5249058331429932No Hit
ACAGCGGCGACGATACTCTGGGGG6100060.4118542922458254No Hit
ACGGAGACGAAGTGCATGAT5886700.3974489861023499No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACTG5698680.3847545463709276No Hit
AGCGGCGACGATACTCTGGGGG5327340.35968299414315197No Hit
AAGTTCTGACGGTACCTGATG5242340.3539440973387115No Hit
AGGCGGGAGAGAGCAAACCTAGTGAACTG5025960.3393348915675959No Hit
ATAGGCGGGAGAGAGCAAACCTAGTGAACTG4863350.3283560443985363No Hit
TCACATCGACGCGGAGGTTTGGCACC4277870.28882652320955027No Hit
AAGTGATGTACCGAAGCTGTGGAC4168190.28142132083883226No Hit
TGACAGCGGCGACGATACTCTGGGGG3848930.25986602444135387No Hit
ATGAGCTGTGGTTAGGGG3633230.24530272984467374No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAC3541810.2391303775404155No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAG3398580.22945999884276835No Hit
ATCAGTAGCTGGTCTGAGAGGATG3397970.22941881381864238No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCT3321440.22425178120165612No Hit
AGGAAGCTTGACTGTGAGACTG3297100.22260843122259635No Hit
TCTCGGAGAGAGGCTCGGCGAAATAGG3295560.2225044559157865No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAG3162810.21354164943591034No Hit
AGTTCAGATTGCAGGCTA3150600.21271727378906072No Hit
ATAAAAGTTACTCTAGGGA3116620.21042306539594438No Hit
TCACATCGACGCGGAGGT2837820.19159948387737963No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGG2637420.1780691907054988No Hit
AGTGATGTACCGAAGCTGTGGAC2557050.17264289498581784No Hit
CGCGGGATAGAGCAGTCTGGT2536670.17126691008141198No Hit
AGCTTGACTGTGAGACTG2523010.17034463560672192No Hit
ATGGATGAAGTCGAGGAGT2415330.16307446610199075No Hit
AATGGATGAAGTCGAGGAGT2412370.16287461746033022No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCT2407520.16254716276031214No Hit
ACGATGGATACTAGGCGTGGCTTGT2406130.16245331491845127No Hit
AAGGAGGTGGGAGTGCATTGACAACC2360480.1593711897523018No Hit
ATGTGACAGCGGCGACGATACTCTGGGGG2287520.15444519080110206No Hit
ATAAAAGTTACTCTAGGG2231780.15068182482604897No Hit
TCTCGGAGAGAGGCTCGGCG2140970.14455065754591673No Hit
AGTAGCTGGTCTGAGAGG2116970.14293026315407473No Hit
AAACTGACACTGAGGGACG2023050.13658911976733296No Hit
AAGCTTGACTGTGAGACTG2015970.13611110342173957No Hit
ATACGGAGGATGCAAGCG2002000.13516789885282152No Hit
AGTACAGTAGGGGTAGCAGG1999100.13497210119714062No Hit
AAGTGATGTACCGAAGCTGTGGACTC1991490.13445830114206073No Hit
GCGGAACTGGCGGAATTGGTAGACGCGCTAGAC1891550.1277107088286986No Hit
ACATCGACGCGGAGGTTTGGCACC1878840.12685257496535227No Hit
TGTGACAGCGGCGACGATACTCTGGGGG1750840.11821047154219483No Hit
AGGCGGGAGAGAGCAAACCTAGTGAACT1694970.11443832843085261No Hit
TAGGCGGGAGAGAGCAAACCTAGTGAACT1687880.11395963692092928No Hit
AGGAGGGTAAGTCGAACTAG1647520.11123467368531496No Hit
TCGGAGAGAGGCTCGGCGAAATAGG1596870.10781496635419835No Hit
GCGGACGTGGCGGAATTGGTAGACGCGCTAGAG1578200.10655443455021127No Hit
TCAGTAGCTGGTCTGAGAGGATG1574840.10632757933535338No Hit
ATGTACACGGAGTCGACCCGCAACGCGA1533190.10351551990117755No Hit
AACTGACACTGAGGGACG1527380.1031232494254858No Hit
AATACGGAGGATGCAAGCG1519080.10256286303164043No Hit
AACGATGGATACTAGGCGTGGCTTGT1515460.10231845354420425No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)