FastQCFastQC Report
Mon 26 Jun 2023
ERR601608.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR601608.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26028360
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length49
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGAGCATGTCTGCCTCAGCGTCGGGTGGAATTCTCGGGTGCCAAGGA1905830.7322128631999865RNA PCR Primer, Index 1 (100% over 22bp)
GTGAATCATCGAATCTTTGAACGCATATTGCGCTCGTGGAATTCTCGGG1226890.47136661702850274No Hit
ACCACGTGCGAACCCTCAGTGACGATCTGTTGGAATTCTCGGGTGCCAA852070.32736215420410664No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGCGATGGAATTCTCGGGTG835950.3211689096047542No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGG812430.31213261227368916Illumina Small RNA Adapter 2 (100% over 21bp)
ACGGGGAGGTAGTGACAATAAATAACATGGAATTCTCGGGTGCCAAGGA806360.3098005406410546RNA PCR Primer, Index 1 (100% over 22bp)
GGTAACGGAGGCGTGCAAAGGTTCCCTTGGAATTCTCGGGTGCCAAGGA771950.2965803454385908RNA PCR Primer, Index 1 (100% over 22bp)
AGGTAACGGAGGCGTGCAAAGGTTCCCTTGGAATTCTCGGGTGCCAAGG671290.257907144360997Illumina Small RNA Adapter 2 (100% over 21bp)
TTGGGCGTAAAGCGTCTGTAGGTGGCTCGTGGAATTCTCGGGTGCCAAG653630.2511222374363963No Hit
GGGTCGTAAACTGCTTTTCTCAGAGAAGAAGTGGAATTCTCGGGTGCCA651120.2501579046855046No Hit
TGCTGGGCCGAAACTGACACTGAGAGACGTGGAATTCTCGGGTGCCAAG589280.22639920455994922No Hit
GAGATTCATCCCGTGTTGCTAAGATTTGTTGGAATTCTCGGGTGCCAAG575790.2212163962693001No Hit
TAATACCCCATACAGCTGAGGAGTGAAAGGTGTGGAATTCTCGGGTGCC569470.2187882755578915No Hit
AAACAGCAGGACGGTGGTCATGGAAGTTGGAATTCTCGGGTGCCAAGGA556840.21393587609822518RNA PCR Primer, Index 1 (100% over 22bp)
CGCCCATTAAAGTGGTACGTGAGCTGGGTTTGGAATTCTCGGGTGCCAA546760.21006317724205445No Hit
AATGTAGATAAGGGAAGTCGGCAAAATTGGAATTCTCGGGTGCCAAGGA525780.20200273855133402RNA PCR Primer, Index 1 (100% over 22bp)
AGGATGTTGACACAATGTGATTTCTGCCCATGGAATTCTCGGGTGCCAA498070.19135665866001544No Hit
TGAACTGTCTTGGGGTAAAGCTACTGTTTTGGAATTCTCGGGTGCCAAG490550.18846750237049126No Hit
AGCGGAGGAAAAGAAACTAACAAGGATTCCCCTGGAATTCTCGGGTGCC477940.1836227868371269No Hit
AACGCAGGTGTCCTAAGATGAGCTCAACGAGAACTGGAATTCTCGGGTG474120.18215515691345902No Hit
AGCGGCGAGCGAACCGGGAACAGCCTAAACCTGGAATTCTCGGGTGCCA466950.17940046933421852No Hit
AACCCTGCACCATCGACCGACCATGTGGAATTCTCGGGTGCCAAGGAAC466850.1793620497027089RNA PCR Primer, Index 1 (100% over 24bp)
AGGTGTCCTAAGATGAGCTCAACGAGAACTGGAATTCTCGGGTGCCAAG457420.17573907845135076No Hit
CTCTGGTGGAGGCTCGTAGATGTGCTGACTGGAATTCTCGGGTGCCAAG440200.16912321790539242No Hit
CAATGGGAAACGCTGGGTAGCTACGCATGGATTGGAATTCTCGGGTGCC435740.16740970234006292No Hit
AAGAGCATGTCTGCCTCAGCGTCGGGTGGAATTCTCGGGTGCCAAGGAA430410.16536193598059962RNA PCR Primer, Index 1 (100% over 23bp)
TCCACGAGTCGGGTTGTTTGGGAATGCAGCCCTTGGAATTCTCGGGTGC425140.16333722140004212No Hit
GGTGTCCTAAGATGAGCTCAACGAGAACTGGAATTCTCGGGTGCCAAGG413490.15886133432917018Illumina Small RNA Adapter 2 (100% over 21bp)
GCGGTGAATCATGATAACTTCACGAATCGTATGGAATTCTCGGGTGCCA410980.15789700157827846No Hit
ATGGATCGTTCATACCTTCATGAAAACTCCCCTGGAATTCTCGGGTGCC410840.157843214094165No Hit
AGGAAGCTAAACTCAAGATGAGTGCTCTCTGGAATTCTCGGGTGCCAAG410790.15782400427841017No Hit
TGGCGAACGGGTGCGTAACGCGTAAGAACCTTGGAATTCTCGGGTGCCA407970.1567405706698386No Hit
CGCAGGTGTCCTAAGATGAGCTCAACGAGAACTGGAATTCTCGGGTGCC393700.15125808925341436No Hit
GACATTGTCAGGTGGGGAGTTTGGCTGGGTGGAATTCTCGGGTGCCAAG382310.14688209322446746No Hit
ATTAAGGCGTACGGTGGAGACCTAGGCACTCTGGAATTCTCGGGTGCCA372550.14313233718912755No Hit
AGCTGAGGAGTGAAAGGTGTGGAATTCTCGGGTGCCAAGGAACTCCAGT360630.13855271711317962RNA PCR Primer, Index 1 (100% over 30bp)
TGTGCGGATGCTGGAGCATATGGAATTCTCGGGTGCCAAGGAACTCCAG354770.1363013267067153RNA PCR Primer, Index 1 (100% over 29bp)
ATCAGGTCTCCAAGGTGAACAGCCTCTTGGAATTCTCGGGTGCCAAGGA354360.1361438062175258RNA PCR Primer, Index 1 (100% over 22bp)
TTAGTGACGCGCATGAATGGATTTGGAATTCTCGGGTGCCAAGGAACTC352130.13528704843486103RNA PCR Primer, Index 1 (100% over 26bp)
ACGCTCTGAATACATTAGCATGGAATAACACGATGGAATTCTCGGGTGC345480.13273214293947064No Hit
TTGGACAAGGCGGCTGGCGGTAACGCAAGCGAGCCCTGGAATTCTCGGG343460.1319560663829761No Hit
CGCCCATTAAAGTGGTACGTGAGCTGGGTTGGAATTCTCGGGTGCCAAG340340.13075737387987563No Hit
AAGACTGAAGTGGAGAAAGGTTCCATGTGGAATTCTCGGGTGCCAAGGA330160.12684625539219527RNA PCR Primer, Index 1 (100% over 22bp)
GTCGTCATCGTATAGTGGTCAGTATCCCTGCCTTGGAATTCTCGGGTGC325340.1249944291534311No Hit
AAGGCTAAATACTGCCGAGAGACCGATAGCGAACTGGAATTCTCGGGTG322990.1240915678129548No Hit
AAAACAAAGCATTGTGATGGTCCTAAAGGATGTTTGGAATTCTCGGGTG319120.1226047280735321No Hit
TGAGATTCATCCCGTGTTGCTAAGATTTGTTGGAATTCTCGGGTGCCAA317760.12208222108500114No Hit
GACACAATGTGATTTCTGCCCAGTGCTCTGTGGAATTCTCGGGTGCCAA304760.11708766898874919No Hit
TGACCTGTACCTGAAACCGACACAGGTAGGTTGTGGAATTCTCGGGTGC303550.11662279144748268No Hit
ATGAAGCTTGACTGTATCTTGGAATTGGTGGAATTCTCGGGTGCCAAGG299270.11497843121887048Illumina Small RNA Adapter 2 (100% over 21bp)
AAAGGCAAAAGGGAGCTTGACTGCAAGACCTTGGAATTCTCGGGTGCCA297680.11436755907786736No Hit
TCCACGAGTCGGGTTGTTTGGGAATGCTGGAATTCTCGGGTGCCAAGGA289400.11118641358886998RNA PCR Primer, Index 1 (100% over 22bp)
AAGATTTGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCA289230.11112110021530362RNA PCR Primer, Index 3 (100% over 40bp)
ACCACGTGCGAACCCTCAGTGACGATCTGTGGAATTCTCGGGTGCCAAG281370.10810131717864668No Hit
CAAGAGCATGTCTGCCTCAGCGTCGGGTTGGAATTCTCGGGTGCCAAGG273650.10513532162610321Illumina Small RNA Adapter 2 (100% over 21bp)
AAACAGCAGGACGGTGGTCATGGAAGTCTGGAATTCTCGGGTGCCAAGG272690.10476649316361077Illumina Small RNA Adapter 2 (100% over 21bp)
CCTAATGGGGATCCTTGAATCAGGACCCTTGACTGGAATTCTCGGGTGC270030.1037445309654546No Hit
AAGGCGTACGGTGGAGACCTAGGCACTCTGGAATTCTCGGGTGCCAAGG267490.10276867232511Illumina Small RNA Adapter 2 (100% over 21bp)
ACCCAATGATGTCACCCGACTAAGGATACATCCGCCTGGAATTCTCGGG265930.10216932607355976No Hit
AGGAGGGCGTGGGTGTCGAGACTTGGAATTCTCGGGTGCCAAGGAACTC264620.1016660289007836RNA PCR Primer, Index 1 (100% over 26bp)
AAGTAGGAAGCTAAACTCAAGATGAGTGCTCTCTGGAATTCTCGGGTGC261860.10060564707111781No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)