FastQCFastQC Report
Mon 26 Jun 2023
ERR601610_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR601610_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38543800
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA3396040.881085933405632No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG2957500.7673088797679523No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC2779770.7211977023542048No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGC2516900.6529973692266979No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTGC2439880.6330149077153784No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA2286810.5933016464385972No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTGGAATTCTCGGGTGCC2213140.5741883260083334No Hit
AGGTCGGTTTCTATCTATTTACGATTTCTCTGGAATTCTCGGGTGCCAAG1932430.5013594923178306No Hit
TGGAATGAGTCCACTTTAAATCCTTTAACGATGGAATTCTCGGGTGCCAA1705950.4426003663364796No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAA1369360.3552737405237678No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAAG1130460.2932923064150395No Hit
CAGTGCGCCCCGGGCGTCGTCGCGCCGTCGGGTCTGGAATTCTCGGGTGC972710.2523648420757683No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA922720.23939518158562467No Hit
TTGGATAACTGTGGTAATTCTAGAGCTAATACATGCTGGAATTCTCGGGT871340.22606489240811753No Hit
CCTCGGTTGGCCCCGGATAGCCGGGTCCCCGTGGAATTCTCGGGTGCCAA849740.22046087827354852No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAG794570.20614729217150357No Hit
ATGTACACGGAGTCGACCCGCAACGCGATGGAATTCTCGGGTGCCAAGGA758210.19671386837831245RNA PCR Primer, Index 1 (100% over 22bp)
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA753070.1953803205703641No Hit
CGCTGCGATCTATTGAAAGTCAGCCCTCGACACATGGAATTCTCGGGTGC749550.19446707382250844No Hit
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGC708550.18382982477078025No Hit
AGCCCGTGGACGGTGTGAGGCCGGTAGCGGCCCCCGTGGAATTCTCGGGT695340.1804025550153332No Hit
GCCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGA691790.17948152491451283RNA PCR Primer, Index 1 (100% over 22bp)
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG674770.17506576933255152No Hit
GAGGTGGAGCACGAGCGTACGCGTTAGGACCCGATGGAATTCTCGGGTGC631210.16376434082783742No Hit
AACGATTAAAGTCCTACGTGATCTGAGTTTGGAATTCTCGGGTGCCAAGG615730.15974813069806298Illumina Small RNA Adapter 2 (100% over 21bp)
TCGTTTTTTCACTGACCCGGTGAGGCGGGGTGGAATTCTCGGGTGCCAAG610500.15839123283122059No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAAGG600320.15575008172520613Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTGGAATTCTCGGGTGCCAAG578640.15012531198273132No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGC576740.14963236629496832No Hit
TTGGTGACTCTAGATAACCTCGGGCCGATCGCATGGAATTCTCGGGTGCC572660.14857383029177196No Hit
ACGGTGGCCATGGAAGTCGGAATCCGCTTGGAATTCTCGGGTGCCAAGGA558670.14494419335924325RNA PCR Primer, Index 1 (100% over 22bp)
ACGTACGCAGTTTTATCCGGTAAAGCGAATGTGGAATTCTCGGGTGCCAA525010.13621127133287325No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA505730.13120916982757277No Hit
TACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCTGGAATTCTCGGG484480.1256959614775917No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTCTGGAATTCTCGGGTGC473910.12295362678303645No Hit
CGGCGGCGTCCGGTGAGCTCTCGCTGGCCCTTGGAATTCTCGGGTGCCAA467620.12132171711144206No Hit
GGTCGGTTTCTATCTATTTACGATTTCTCTGGAATTCTCGGGTGCCAAGG462830.12007897508808162Illumina Small RNA Adapter 2 (100% over 21bp)
TGCCCTCGGCCGATCGAAAGGGAGTCGGGTTTGGAATTCTCGGGTGCCAA454780.11799044204255937No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG453420.11763759670816058Illumina Small RNA Adapter 2 (100% over 21bp)
TAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCAA449910.11672694441129312No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAATTCTCGGGTGCCAAGGAA445580.1156035471333911RNA PCR Primer, Index 1 (100% over 23bp)
CAGTCCGCCCGGAGGATTCAACCCGGCGGCGCGTGGAATTCTCGGGTGCC438560.11378224253965619No Hit
ATTCCCCATGAACGAGGAATTCCCAGTAAGTGCGGGAATTCTCGGGTGCC430770.11176116521982783No Hit
TACCCGTCGCCGCGCTCTCCCTCTCCCCTGGAATTCTCGGGTGCCAAGGA408190.10590289488841267RNA PCR Primer, Index 1 (100% over 22bp)
CAAAAAGTAAGTTTAGATTATAGCCAAAAGTGGAATTCTCGGGTGCCAAG407690.10577317233900134No Hit
CAAAAAGTAAGTTTAGATTATAGCCAAAAGAGGTGGAATTCTCGGGTGCC405810.10528541555321479No Hit
CTGGGTCGGGGTTTCGTACGTAGCAGAGCAGCTTGGAATTCTCGGGTGCC400560.10392332878439592No Hit
ATAATCCACCTATAACTTCTCTGTTAACCCAATGGAATTCTCGGGTGCCA398160.10330066054722159No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)