FastQCFastQC Report
Sat 5 Aug 2023
ERR6109833_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6109833_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66550236
Total Bases5 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATGC3980160.5980685027172556RNA PCR Primer, Index 16 (100% over 50bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATGTCGTATGC865030.12998150750359472RNA PCR Primer, Index 16 (98% over 50bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCATCTCGTATG820180.12324223763834587RNA PCR Primer, Index 16 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCCGTCCAGCTCGTATGC714920.10742561453876737RNA PCR Primer, Index 16 (98% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)