FastQCFastQC Report
Mon 26 Jun 2023
ERR6109836_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6109836_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74382591
Total Bases5.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AATAGATCGCAGCGAGGGAGCTGCTCTGCTTGGAATTCTCGGGTGCCAAG24857983.34190832368289No Hit
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC21497722.8901547675315586RNA PCR Primer, Index 1 (100% over 50bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGT16191512.1767875765446245No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCA13119851.763833421720951No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGCC11006351.47969435482558No Hit
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATG10318511.387221103927396RNA PCR Primer, Index 1 (100% over 49bp)
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCTGGAATTCTCGGGTGCC9957471.3386828646504125No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGC9654191.2979098832413622No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTG9603641.2911139382063204No Hit
AATGATAGGAAGAGCCGACATCGAAGGATCTGGAATTCTCGGGTGCCAAG7145460.9606360714162269No Hit
AAACGCTTCGGGCCCCGCGGGACACTCAGCTTGGAATTCTCGGGTGCCAA6684520.8986672701412082No Hit
AGGTTGAGATCGTTTCGGCCCCAAGACCTCTTGGAATTCTCGGGTGCCAA6074950.8167166427423859No Hit
AAAAAGCGACGTCGCTATGAACGCTTGGCCGCCACTGGAATTCTCGGGTG5505630.7401772277601892No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCCAA5462790.7344178155880587No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCAACTTGGAATTCTCGGGTGC4871480.6549220636855739No Hit
AGACAAATCGCTCCACCAACTAAGAACGGCCTGGAATTCTCGGGTGCCAA4592440.6174079093319027No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTACTTGGAATTCTCGGGTGC4525240.6083735372971882No Hit
AGGTCGGACGACCGATTTGCACGTCAGGACCGCTTGGAATTCTCGGGTGC3683460.49520458355638614No Hit
AAGATCAAGCGAGCTTTTGCCCTTCTGCTCCTGGAATTCTCGGGTGCCAA3467400.46615746418405885No Hit
ACCAACTAAGAACGGCCATGCACCACCACCCTGGAATTCTCGGGTGCCAA3382290.454715270674021No Hit
ATCCTGAGGGAAACTTCGGAGGGAACCAGCTTGGAATTCTCGGGTGCCAA3301000.4437866381933375No Hit
AACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCTGGAATTCTCGGGTG3260450.4383350937586995No Hit
ATCGGAATTAACCAGACAAATCGCTCCACCTGGAATTCTCGGGTGCCAAG3127850.42050834179734337No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCTGGAATTCTCGGGTGCCAA3096600.4163070899210811No Hit
AAGGCACGGGCCCCTCTCTCGGGGCGAACCCTGGAATTCTCGGGTGCCAA3081350.4142568790054651No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTGGAATTCTCGGGTG3063680.41188132314455134No Hit
AAACCCTTGTGTCGAGGGCTGACTTTCAATTGGAATTCTCGGGTGCCAAG3004230.40388886157515No Hit
AGCTATCCTGAGGGAAACTTCGGAGGGAACCTGGAATTCTCGGGTGCCAA2878790.38702470044368315No Hit
ACAATGATAGGAAGAGCCGACATCGAAGGATCTGGAATTCTCGGGTGCCA2783520.3742165959236349No Hit
AACCAGACAAATCGCTCCACCAACTAAGAACGGCCTGGAATTCTCGGGTG2762630.3714081430693911No Hit
AGATCGCAGCGAGGGAGCTGCTCTGCTACGTTGGAATTCTCGGGTGCCAA2507290.3370802181386771No Hit
AAACAGTCGGATTCCCCTGGTCCGCACCAGTTCTTGGAATTCTCGGGTGC2474620.33268806137715745No Hit
ACCGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCTGGAATTCTCGGGT2464780.33136517118636No Hit
CGTTGAACCCCATTCGTGATGGGGATCGGGGATTTGGAATTCTCGGGTGC2275750.30595196663692453No Hit
CCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG2235450.3005340322172967RNA PCR Primer, Index 1 (100% over 45bp)
AAGAGCTAATAGAAAGGCTAGGACCAAACCTTGGAATTCTCGGGTGCCAA2220160.29847844369927906No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATGGAATTCTCGGGTGCC2220070.2984663440938754No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGATGGAATTCTCGGGTGCC2205870.2965572952413018No Hit
ACCGGCCTCACACCGTCCACGGGCTGGGCCTCGATCTGGAATTCTCGGGT2197640.29545085354716943No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAG2129020.2862255766271976No Hit
ACCGGGGCCCGCGCGGCCAACCCCCGGGACGGGGACTGGAATTCTCGGGT2122570.2853584382399371No Hit
AAAGTAAACGCTTCGGGCCCCGCGGGACACTCTGGAATTCTCGGGTGCCA2020570.271645552115817No Hit
ATTGGAGCTGGAATTACCGCGGCTGCTGGCACCTGGAATTCTCGGGTGCC1974560.2654599649533585No Hit
ACGGAAACCTTGTTACGACTTTTACTTCCTCTTGGAATTCTCGGGTGCCA1962940.26389777145568916No Hit
AGTCGGATTCCCCTGGTCCGCACCAGTTCTTGGAATTCTCGGGTGCCAAG1936790.26038216388563284No Hit
AGGTAGGGACAGTGGGAATCTCGTTCATCCTGGAATTCTCGGGTGCCAAG1919540.25806307284993607No Hit
CCCGGGGCTACGCCTGTCTGAGCGTCGCTTGGAATTCTCGGGTGCCAAGG1908570.256588265391293Illumina Small RNA Adapter 2 (100% over 21bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTGGAATTCTCGGGTGC1810880.24345481592594698No Hit
ACGCTGAGCCAGTCAGTGTAGCGCGCGTGCTGGAATTCTCGGGTGCCAAG1763940.23714419950765092No Hit
ACTGGCAGGATCAACCAGGTAGGTAGGCTGGAATTCTCGGGTGCCAAGGA1760460.23667634809871035RNA PCR Primer, Index 1 (100% over 22bp)
ATCCAGGCGGCTCGGGCCTGCTTTGAACACTCTTGGAATTCTCGGGTGCC1757100.23622462949697462No Hit
ATGCTACTGGCAGGATCAACCAGGTAGGTAGGCTGGAATTCTCGGGTGCC1750050.23527682707368985No Hit
ACAAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGG1736910.23351028468475912Illumina Small RNA Adapter 2 (100% over 21bp)
ACCGCGGCTGCTGGCACCAGACTTGCCCTCCTGGAATTCTCGGGTGCCAA1719780.23120732645626718No Hit
AGGGCAGACGTTCGAATGGGTCGTCGCCGCCTGGAATTCTCGGGTGCCAA1661200.22333182773910093No Hit
ATCCGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTTGGAATTCTCGGGTG1549800.20835520505060115No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCTGGAATTCTCGGGTGCCAA1519430.2042722604271744No Hit
ACGTACGAAACCCCGACCCAGAAGCAGGTCGTCTTGGAATTCTCGGGTGC1516390.2038635626446516No Hit
ACGACGGTCTAAACCCAGCTCACGTTCCCTTGGAATTCTCGGGTGCCAAG1486470.19984111604824306No Hit
AGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCTGGAATTCTCGGGTGC1480280.19900893207659304No Hit
CGACATCGAAGGATCAAAAAGCGACGTCGCTTGGAATTCTCGGGTGCCAA1454630.19556054453655694No Hit
TCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCT1451900.19519352317264668RNA PCR Primer, Index 1 (100% over 50bp)
AGGTTGAGATCGTTTCGGCCCCAAGACCTCTAATCTGGAATTCTCGGGTG1379250.18542645281071213No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1369100.18406188620130212No Hit
AAGTAGGAGAGGAGCGAGCGACCAAAGGAACCTGGAATTCTCGGGTGCCA1334160.1793645505034908No Hit
ATACCCAGGTCGGACGACCGATTTGCACGTCTGGAATTCTCGGGTGCCAA1322200.1777566473854077No Hit
CGGCCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1297810.1744776543210225RNA PCR Primer, Index 1 (100% over 45bp)
CCCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC1265570.17014330678532025RNA PCR Primer, Index 1 (100% over 44bp)
TCGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCTGGAATTCTCGGGTGCC1243590.16718831426563238No Hit
ACAAAGGGCAGGGACTTAATCAACGCAAGCTTTGGAATTCTCGGGTGCCA1231190.16552125752113153No Hit
GTTGAACCCCATTCGTGATGGGGATCGGGGATTGGAATTCTCGGGTGCCA1208030.16240762573059603No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCCTGGAATTCTCGGGTGCCA1196390.16084274343172586No Hit
CCGGTTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCG1124420.15116709231061876RNA PCR Primer, Index 1 (100% over 45bp)
AGTTATCCAAGTAGGAGAGGAGCGAGCGACCTGGAATTCTCGGGTGCCAA1094120.14709355849139485No Hit
ACGAAACCCCGACCCAGAAGCAGGTCGTCTTGGAATTCTCGGGTGCCAAG1088300.14631111734195978No Hit
ACGGTCTGTATTCGTACTGAAAATCAAGATCTGGAATTCTCGGGTGCCAA1073540.14432678205576355No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTTTGGAATTCTCGGGTGCCAA1058340.14228329314314958No Hit
AAATCGCTCCACCAACTAAGAACGGCCTGGAATTCTCGGGTGCCAAGGAA1050750.141262893087443RNA PCR Primer, Index 1 (100% over 23bp)
AGGTTCCCCACGAACGTGCGGTGCGTGACGGGCTGGAATTCTCGGGTGCC1049630.14111232022019776No Hit
AAAGGGCAGGGACTTAATCAACGCAAGCTTTGGAATTCTCGGGTGCCAAG1045450.14055036076922892No Hit
ACACTCCTTAGCGGATTCCGACTTCCATGGCCTGGAATTCTCGGGTGCCA1044430.1404132319079877No Hit
ACTCCTTAGCGGATTCCGACTTCCATGGCCTGGAATTCTCGGGTGCCAAG1043380.1402720698449453No Hit
AGGTTCCCCACGAACGTGCGGTGCGTGACTGGAATTCTCGGGTGCCAAGG1006660.13533543084026206Illumina Small RNA Adapter 2 (100% over 21bp)
TTTGGTGACTCTAGATAACCTCGGGCCGATCGCACTGGAATTCTCGGGTG1003240.13487564583492392No Hit
AGGACCGACTGACCCATGTTCAACTGCTGTTCTGGAATTCTCGGGTGCCA995890.13388751139362703No Hit
CGGACGCCGCCGGAACCGCGACGCTTTCCAAGGCTGGAATTCTCGGGTGC994100.13364686368615475No Hit
ATGTCCCGCGCCCCGCCGCGGGGCGGGGATTCGGCGCTGGAATTCTCGGG991760.13333227394566022No Hit
ACGGCGACTACCATCGAAAGTTGATAGGGCTGGAATTCTCGGGTGCCAAG945710.12714130918080013No Hit
AAAGTAAACGCTTCGGGCCCCGCGGGACACTCAGCTTGGAATTCTCGGGT933720.1255293728609158No Hit
ACTTTGACATTCAGAGCACTGGGCAGAAATCTGGAATTCTCGGGTGCCAA928940.12488674937392273No Hit
ACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCTGGAATTCTCGGGTGCCA927840.1247388653078783No Hit
CGTGGGGGGCCCAAGTCCTTCTGATCGAGGCCCTGGAATTCTCGGGTGCC923340.12413388503769653No Hit
AAGCTTGGGCTGCAGGTCGACCCGTGCGGAGGAGCTGGAATTCTCGGGTG915180.12303685414776692No Hit
AATAGATCGCAGCGAGGGAGCTGCTCTGCTACGTTGGAATTCTCGGGTGC911820.1225851355460312No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCTGGAATTCTCGGGTGCCAA910280.12237809785356898No Hit
AAATCGCTCCACCAACTAAGAACGGCCATGCACCTGGAATTCTCGGGTGC909600.12228667861274153No Hit
CGAGGCCAACCGAGGCTCCGCGGCGCTGCCGTTGGAATTCTCGGGTGCCA904960.1216628767341541No Hit
AATTAAACAGTCGGATTCCCCTGGTCCGCACCTGGAATTCTCGGGTGCCA896110.12047308220279661No Hit
AGTAGGGTAAAACTAACCTGTCTCACGACGGTCTTGGAATTCTCGGGTGC890900.11977264948998616No Hit
AGAAGGACTTGGGCCCCCCACGAGCGGCGCTGGAATTCTCGGGTGCCAAG880110.11832204124215032No Hit
ACGGATCCGGCTTGCCGACTTCCCTTACCTTGGAATTCTCGGGTGCCAAG877500.11797115268544492No Hit
AGCGCCAGGTTCCCCACGAACGTGCGGTGCGTGACTGGAATTCTCGGGTG856490.11514656702399624No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGGAATTCTCGGGTGCCAAG824190.11080415308469156No Hit
ACGGATCCGGCTTGCCGACTTCCCTTACCTACTGGAATTCTCGGGTGCCA800780.10765691127914594No Hit
ACCGGCTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTC783500.10533378704164796RNA PCR Primer, Index 1 (100% over 44bp)
AAGCTTGGGCTGCAGGTCGACCCGTGCTGGAATTCTCGGGTGCCAAGGAA782970.10526253380982654RNA PCR Primer, Index 1 (100% over 23bp)
AAGAGCTAATAGAAAGGCTAGGACCAAACCTCTGGAATTCTCGGGTGCCA773600.10400283044724806No Hit
CGGACGCCGCCGGAACCGCGACGCTTTCCTGGAATTCTCGGGTGCCAAGG746110.10030707319673766Illumina Small RNA Adapter 2 (100% over 21bp)
AGAGTCTCGTTCGTTATCGGAATTAACCAGACTGGAATTCTCGGGTGCCA746030.10029631799193443No Hit
ATGCCAGAGTCTCGTTCGTTATCGGAATTAACCTGGAATTCTCGGGTGCC745610.10023985316671748No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)