FastQCFastQC Report
Thu 29 Jun 2023
ERR6109837_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6109837_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77326257
Total Bases5.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATG11595171.4995126429047252RNA PCR Primer, Index 3 (100% over 49bp)
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC5112120.6611104944598573RNA PCR Primer, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)