FastQCFastQC Report
Thu 29 Jun 2023
ERR6109838_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6109838_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47487118
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGC2796410.5888775983415123RNA PCR Primer, Index 3 (100% over 50bp)
GGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGC1149880.2421456699056784RNA PCR Primer, Index 3 (100% over 50bp)
TCGGGTGCCAAGGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCT1030380.21698095049693267RNA PCR Primer, Index 3 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)