FastQCFastQC Report
Sat 24 Jun 2023
ERR6109839_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6109839_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50335174
Total Bases3.8 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGC2678070.5320474306893227RNA PCR Primer, Index 1 (100% over 50bp)
TCGGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCT1177350.23390204233723322RNA PCR Primer, Index 1 (100% over 50bp)
GGGTGCCAAGGAACTCCAGTCACATCACGATCTCGTATGCCGTCTTCTGC1151040.2286750811668993RNA PCR Primer, Index 1 (100% over 50bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)