FastQCFastQC Report
Thu 29 Jun 2023
ERR6109859_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6109859_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56347022
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGC4308790.7646881498014216RNA PCR Primer, Index 11 (100% over 50bp)
GGATACACAATTCTGAGGGATCATAGCATCTCCATTTTGGAAAAAAGTTA3915480.6948867679289245No Hit
TACACATGGCATGGATGAACTATACAAACCCGGGATTTTGGAAAAAAGTT977790.17353002258043024No Hit
TCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATGCCGTCTTCT877580.15574558669666697RNA PCR Primer, Index 11 (100% over 50bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACGGCTACATCTCGTATG761080.13507013733574066RNA PCR Primer, Index 11 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)