FastQCFastQC Report
Thu 29 Jun 2023
ERR6109862_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6109862_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60538968
Total Bases4.6 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGC8283711.36832692622048RNA PCR Primer, Index 12 (100% over 50bp)
GGATACACAATTCTGAGGGATCATAGCATCTCCATTTTGGAAAAAAGTTA2339770.3864899051467148No Hit
TCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCT1965620.3246867373094302RNA PCR Primer, Index 12 (100% over 50bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG1417990.2342276465631195RNA PCR Primer, Index 12 (100% over 49bp)
GGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATGCCGTCTTCTGC919200.15183608679949748RNA PCR Primer, Index 12 (100% over 50bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACCTTGTAATCTCGTATG813370.13435478450838476RNA PCR Primer, Index 12 (100% over 49bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)