FastQCFastQC Report
Thu 29 Jun 2023
ERR6109864_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6109864_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62921893
Total Bases4.7 Gbp
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGC10354791.6456577363303422RNA PCR Primer, Index 10 (100% over 50bp)
GGATACACAATTCTGAGGGATCATAGCATCTCCATTTTGGAAAAAAGTTA2961370.47064222940654377No Hit
TCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCT2633530.41853953758193513RNA PCR Primer, Index 10 (100% over 50bp)
CTGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG1724810.2741192163433481RNA PCR Primer, Index 10 (100% over 49bp)
GGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGC1003440.15947390521133875RNA PCR Primer, Index 10 (100% over 50bp)
ATGGAATTCTCGGGTGCCAAGGAACTCCAGTCACTAGCTTATCTCGTATG944150.15005111178075967RNA PCR Primer, Index 10 (100% over 49bp)
TACACATGGCATGGATGAACTATACAAACCCGGGATTTTGGAAAAAAGTT859160.13654388942176296No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)