FastQCFastQC Report
Sat 24 Jun 2023
ERR6133623_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133623_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7045629
Total Bases204.3 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGCGAAGTCCCGCAACGCGA4864316.904010983263524No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT4351486.176141264321468No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT2876754.083027931218065No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC2386123.3866671094944114No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT1161801.6489656210964272No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA848731.204619204332218No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC702280.9967598350693743No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC697660.9902025780806796No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT624080.8857690349577021No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT546860.7761691681466623No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGA543770.7717834702905872No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA414690.5885776841216022No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT391880.5562030018895403No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT288110.4089201971889238No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT260810.3701727695284551No Hit
ATGTACACGGCGAAGTCCCGCAACGCG254430.3611175098774006No Hit
ATGCCGACTAGGGATCGGGTGGTGT236910.33625102882936353No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC206470.2930469373280938No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGA196870.27942146826067626No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTC192100.2726513133178031No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG189910.2695430031867985No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA174660.24789837784532795No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGATCG153210.2174539703978169No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT151750.21538176364381376No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT142180.2017988741672319No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTT130380.1850509017718645No Hit
AGGGAGGTAGTGACAATAAATAACG127920.18155937532333877No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCT118840.168671952497073No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT117720.16708231443920762No Hit
ATGACCCACTCGGCACCTTACGAGAAATC114270.16218566149310445No Hit
TAGGCCACCATCGAAAGGGAATCCGGTT112930.16028377310244407No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCC112760.16004248875437524No Hit
TTCGACGAGTCGAGTTGTTTGGGAATGC109590.15554324532273842No Hit
ACGCGCGCTACACTGACGGAGCCAGCGAGTA107250.15222203723755537No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTGT105160.149255659076003No Hit
TATCTGGTTGATCCTGCCAGTAGTC104700.14860277201652258No Hit
ACGTGAAATTGTTGAAAGGGAAGGGC103660.14712667953421904No Hit
TTAGGCCACCATCGAAAGGGAATCCGGTT103590.14702732715560243No Hit
AGGGTATTGTAAGCAGTAGAGTAGCC99710.14152036674085453No Hit
AGCCAGCTGGGACTGAGGATTGCGACT95670.13578631517498296No Hit
AAGAGGGCGTGAAACCGTTAAGAGGT95610.1357011559933116No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC92190.1308470826380441No Hit
ATAGCTGTTGGGACCCGAAAGATGGTGAACT90090.1278665112795465No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCC88010.12491432631493939No Hit
TTGATGATTCATAATAACTTTTCGAATCGC87710.12448853040658257No Hit
TAGGGGGCATGCCTGTTTGAGCGTCATTT87660.12441756442185645No Hit
ATGCCAGGTCGTACTGATAACCGCAGC87030.12352339301430716No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATT86710.12306921071205991No Hit
TACACGGCGAAGTCCCGCAACGCGA86470.12272857398537447No Hit
TATGACCCACTCGGCACCTTACGAGAAATC86420.12265760800064834No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT84740.12027315091385027No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTC83580.11862674006820398No Hit
TGTGAAACTCCGTCGTGCTGGGGATAGAGCAT82240.11672485167754364No Hit
ATACGCGAAACTCAGGTGCTGCAATCT80660.11448232656019783No Hit
ATTCCAGGGGGCATGCCTGTTTGAGC77230.10961406000798508No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGT75750.10751346686009154No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAT75280.1068463866036659No Hit
AGGGGATCGAAGATGATCAGATACCGTCGT75230.10677542061893977No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGG73880.10485933903133418No Hit
AAGAGATGGGGAAGCTCCGTTTCAAAGGCCTA73170.1038516220482231No Hit
ACCTATACTCCACCGTCAGGGTTAA70710.10036009559969736No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)