FastQCFastQC Report
Mon 26 Jun 2023
ERR6133624_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133624_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41637465
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length25-32
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGACGAGTCGAGTTGTTTGGGAATGC12111052.9086905266687104No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT5593391.3433550769721452No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG5052461.2134408278698043No Hit
ATGCCGACTAGGGATCGGGTGGTGT3947160.9479827842545169No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT3900430.9367597186812406No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC3747560.9000451876693261No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC3506610.8421766310701191No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT3482440.836371762786231No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGATCG3448950.8283285257639964No Hit
TAGGCCACCATCGAAAGGGAATCCGGTT3087460.7415100799244142No Hit
ATGACCCACTCGGCACCTTACGAGAAATC2890140.6941200671078318No Hit
TTAGGCCACCATCGAAAGGGAATCCGGTT2709510.6507384635447908No Hit
TATGACCCACTCGGCACCTTACGAGAAATC2697230.6477891965805315No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTGT2470790.5934054822982139No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCT2247280.539725461192222No Hit
ATAGCTGTTGGGACCCGAAAGATGGTGAACT2090470.5020646670012212No Hit
AGGGAGGTAGTGACAATAAATAACG2064630.49585871762365935No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATC1878720.4512090253333146No Hit
ATACGCGAAACTCAGGTGCTGCAATCT1789090.42968273885069613No Hit
TTCGACGAGTCGAGTTGTTTGGGAATGC1765710.4240676035392644No Hit
TAGGCCACCATCGAAAGGGAATCCGGTTAAT1741040.41814265109559384No Hit
ATGCCAGGTCGTACTGATAACCGCAGC1708290.41027713862983733No Hit
AAGAGATGGGGAAGCTCCGTTTCAAAGGCCTA1684530.40457073935697097No Hit
ATTGCGATGGTCAGAAAGTGATGTTGACGC1641390.3942098780509332No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT1568970.37681688834802984No Hit
AAGAAGTCCTTGTTGCTTAATTGAACGTGGAC1514050.3636268442375154No Hit
ACGTGAAATTGTTGAAAGGGAAGGGC1477370.35481747027586813No Hit
AGGGTATTGTAAGCAGTAGAGTAGCCT1449440.34810956911041535No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC1448550.3478958193060024No Hit
TTAGGCCACCATCGAAAGGGAATCCGGTTAAT1446270.347348235537394No Hit
ATCAGACACCACAAAAGGTGTTAGTTC1438920.34558299838859063No Hit
AGGGGATCGAAGATGATCAGATACCGTCGT1404270.3372611661156605No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCC1387160.3331518861679019No Hit
ATCAGACACCACAAAAGGTGTTAGTTCATCT1378890.33116569416509867No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCC1370540.32916028869673025No Hit
ATGTACACGGCGAAGTCCCGCAACGCGA1365070.3278465679887092No Hit
AGAATCCATGCTAGAACGCGATGATTTC1356200.32571627499416694No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCCT1353900.3251638878591672No Hit
ATGATGCCCTGACGAGTAGGCAGGCGT1346490.32338424061119No Hit
ATATCAATAAGCGGAGGAAAAGAAACC1332660.3200627127516048No Hit
AGGGTATTGTAAGCAGTAGAGTAGCC1325700.31839114124743184No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCCT1322180.31754574876256275No Hit
ACGATCAACTTAGAACTGGTACGGAC1226510.29456884562977115No Hit
ACGGACAAGGGGAATCTGACTGTCT1185980.28483482363779833No Hit
ATTTGAATGAAGAGCTTTTAGTGGGCC1184780.2845466216543202No Hit
TTGATGATTCATAATAACTTTTCGAATCGC1159530.27848237158530187No Hit
ATGGAGAACAGAAATCTCCAGTAGAAC1120930.2692118744500896No Hit
TTGGCGATGGTTCATTCAAATTTCTGCCCT1119920.26896930444732886No Hit
AACGGCGAGTGAAGCGGCAAAAGCTC1097460.2635751239898971No Hit
AAGTCAGAATCCATGCTAGAACGCGATGATTT1090870.2619924147639632No Hit
ATTGGCGAGAGACCGATAGCGAACAAGT1090580.2619227659512893No Hit
ATGCCGACTAGGGATCGGGTGGTGTT1088180.2613463619843331No Hit
TTGCTGGTTAACTCTTCTTAGAGGGACT1062770.2552436849841843No Hit
AGAATCCATGCTAGAACGCGATGATTT1056380.25370900942216346No Hit
AGACACCACAAAAGGTGTTAGTTCATCT1051350.25250096277475104No Hit
AGCAGGTCTCCAAGGTGAACAGCCTCT1036540.24894406996199217No Hit
TCTTGATGATTCATAATAACTTTTCGAATCGC1012500.24317042355964752No Hit
AGACACCACAAAAGGTGTTAGTTCATCTAGAC1009380.24242109840260448No Hit
ATCAGTTTTATGAGGTAAAGCGAATGATTAGA976500.23452436405530455No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGAG965160.23180085531143646No Hit
ATGATTAATAGGGACGGTCGGGGGCATC962960.23127248500839329No Hit
ACCTATACTCCACCGTCAGGGTTAA960900.23077773827008924No Hit
ACAATGTAAATACCTTAACGAGGAAC955640.22951445290917685No Hit
ATGGAAGTCGGAATCCGCTAAGGAGTGTGT952650.22879634963367726No Hit
TAATGATTAATAGGGACGGTCGGGGGCATC948780.22786689823696038No Hit
ATAGAGGGTGTAGCATAAGTGGGAGCTT947580.2275786962534823No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTG933050.2240890505702016No Hit
TATGGCGTCGCTGAACCATAGCAGGCTGGC931250.22365674759498447No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGC923230.22173059767207248No Hit
AAGAGATGGGGAAGCTCCGTTTCAAAGGCCT917550.2203664416169428No Hit
AGGTAGGAGTACCCGCTGAACTTAAGC908280.2181400812945745No Hit
ACCTTAACGAGGAACAACTGGAGGGC898490.21578883344603234No Hit
ACTGATAACCGCAGCAGGTCTCCAAGGTGAAC896640.2153445220548369No Hit
ACTGCGAAAGCATTTGCCAAGGACGTTTTC887780.21321663074349026No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATC881840.21179003092527365No Hit
AGCTGTTGGGACCCGAAAGATGGTGAACT875060.2101616897186224No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA871900.20940275782879675No Hit
ATAGCAGGCTGGCAACGGTGCTCTT871870.2093955527792098No Hit
ACCCGCTGAACTTAAGCATATCAAT871000.20918660634118816No Hit
ATTGGCGAGAGACCGATAGCGAACAAGTAC869260.20876871346514492No Hit
TCGATCAACTTAGAACTGGTACGGAC864830.2077047678094716No Hit
ATTAAAGTTGTTGCAGTTAAAAAGCTCGT858030.20607162323642902No Hit
ATGCGAGTGTTTGGGTGTAAAACCCGT851300.204455290445756No Hit
ATCGATGAAGAACGCAGCGAATTGCGATATGT838240.20131869219223603No Hit
AAAACGGCGGGAGTAACTATGACTCTCTT832960.20005060346493236No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT826690.1985447481012593No Hit
AGGAGTACCCGCTGAACTTAAGCATATC817610.19636401975960832No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCC814110.19552343064113054No Hit
AGGTCGTACTGATAACCGCAGCAGGTCTCC791850.19017728384761176No Hit
AGCTCTAAGTGGGTGGTAAATTCCATCT788670.18941354859139478No Hit
ATCGATGAAGAACGCAGCGAATTGCGAT788160.1892910627484166No Hit
AAATTCAGGGAGGTAGTGACAATAAATAACG786350.18885635809000378No Hit
ATTCCGGAACCTGGATATGGATTCTTC783530.18817908342883027No Hit
AAATCAGGTAGGAGTACCCGCTGAACTT783090.18807340936822162No Hit
AAATACCTTAACGAGGAACAACTGGAGGGC776510.18649310182548337No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCTAAAT759100.18231177138185525No Hit
AAAAGCTAAATATTGGCGAGAGACCGAT755670.18148799404574703No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC749790.18007580432670434No Hit
AAATATTGGCGAGAGACCGATAGCGAAC748370.1797347653129219No Hit
ATCAATAAGCGGAGGAAAAGAAACC746590.17930726570409605No Hit
AGTTTTAGCGGTTGGATAAATCCTCGGG745910.1791439512467918No Hit
CTTGATGATTCATAATAACTTTTCGAATCGC740560.1778590507371186No Hit
TAATGATTAATAGGGACGGTCGGGGGC735570.17666061082248882No Hit
AAATCAGGTAGGAGTACCCGCTGAACTTAAGC732430.17590648229905448No Hit
AATTCAGGGAGGTAGTGACAATAAATAACG720430.17302446246427347No Hit
ATCAGTTTTAGCGGTTGGATAAATCCTCGGG720030.17292839513644742No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGA712460.17111032095733975No Hit
ATGATGCCCTGACGAGTAGGCAGGC708620.17018807461020982No Hit
ATTCAATTGTCAGAGGTGAAATTCTTGGATTT703780.16902565994351482No Hit
ATTTGCCAAGGACGTTTTCATTAATC703050.1688503370702323No Hit
AAGGACGTTTTCATTAATCAAGAACGAAAGTT693230.16649188417210317No Hit
AGCGAATTGCGATATGTATTGTGAATTGCAGA681290.16362427443649608No Hit
AATGAAAGTGAACGTAGGTGAGGGCCCG673120.16166209926564934No Hit
ACGCGAAACTCAGGTGCTGCAATCT670330.16099202965406276No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCAT664380.1595630281526505No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGAGG648040.15563867781095703No Hit
AAGGTGAACAGCCTCTAGTTGATAGAAT645800.15510070077513124No Hit
ATAGGGGCGAAAGACTAATCGAACC644620.15481730215804443No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATC628040.15083531141965534No Hit
ATTTTGCGCAAGGATAAATCATTTGT623050.14963687150502558No Hit
ATATTAAAGTTGTTGCAGTTAAAAAGCTCGT621500.1492646106096997No Hit
ATCTAAAGCTAAATATTGGCGAGAGACCGAT620460.149014835557352No Hit
ATGAGGTAAAGCGAATGATTAGAGG615740.14788124108900483No Hit
TCGGGCTGATCCGGGTTGAAGACATTGTC611840.146944584642701No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT598380.14371191906135497No Hit
TACGGGCTGATCCGGGTTGAAGACATTGTC593750.14259993974176863No Hit
TAGTTAAAAAGCTCGTAGTTGAACTTTGG588480.14133425269766065No Hit
ACGGTAACGTAACTGAATGTGGAGAC587220.1410316406150086No Hit
ATGGCGTCGCTGAACCATAGCAGGCTGGC582520.13990284951305273No Hit
ACGCGCGCTACACTGACGGAGCCAGCGAGTAC580120.13932644554609652No Hit
ATTGCTCTTCAACGAGGAATTCCTAGT574750.13803674167003202No Hit
AATGATTAATAGGGACGGTCGGGGGCATC573830.13781578681603215No Hit
AGGAGTACCCGCTGAACTTAAGCAT568840.13661734690140237No Hit
ATTGAAGACTAACTACTGCGAAAGC554380.13314451300049127No Hit
AAGCGCAAGTCATCAGCTTGCGTTGATT549870.13206135387925272No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACTT548330.13169149466712252No Hit
TTATGGCGTCGCTGAACCATAGCAGGCTGGC546190.13117753446325323No Hit
AAATGATTAATAGGGACGGTCGGGGGCATC544470.1307644449536013No Hit
TTGGGCCAGCATCAGTTTTAGCGGTTGGAT543840.13061313891227527No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGATC541670.13009197365881903No Hit
AGATGTCCTAAGGGGGACTCATGGAGAAC541120.12995988108305825No Hit
AAAAGTCTTTGGGTTCTGGGGGGAGT534900.12846603413536342No Hit
AAATTGTCAGAGGTGAAATTCTTGGATTT534590.12839158195629824No Hit
ATTCAAATGAGAACTTTGAAGACTGAAGT533810.1282042506670375No Hit
ATGATTAATAGGGACGGTCGGGGGCATCAGT532270.12783439145490724No Hit
ATGAGGTAAAGCGAATGATTAGAGGT529910.12726759422073367No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT527200.12661673807471227No Hit
AGATCCGTAACTTCGGGATAAGGATTGGCTCT519900.12486350934188717No Hit
AGTAACGGCGAGTGAAGCGGCAAAAGCTC515840.12388842596445292No Hit
AAATATGTAAGAAGTCCTTGTTGCTT513950.12343450784047492No Hit
ACAACCTTGGCCGAGAGGTCTGGGT511520.12285089882393176No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT510560.1226203372371493No Hit
ATTTGTATACGACTTAAATGTACAACAGGGT508680.12216882079636694No Hit
ATGATTAATAGGGACGGTCGGGGGC503880.12101601286245453No Hit
TTTGACCTCAAATCAGGTAGGAGTACCC499730.12001931433625941No Hit
AAAGTCTTTGGGTTCTGGGGGGAGT496680.11928680096158592No Hit
ACAAAAGGTGTTAGTTCATCTAGAC493200.11845101520949944No Hit
AAAAAGCTCAAATTTGAAATCTGGCGT491780.118109976195717No Hit
ATTTGTATACGACTTAAATGTACAAC490230.11773771530039112No Hit
AATGTAAATACCTTAACGAGGAACAACT487420.11706284232241324No Hit
ATCAGACACCACAAAAGGTGTTAGTTCA486560.11685629756758727No Hit
ACCAGGTCCAGACACAATAAGGATTGAC482010.11576353171356614No Hit
AAATTACCCAATCCTAATTCAGGGAGGT480910.11549934656204454No Hit
AAGCGTGTTACCTATACTCCACCGTCAGGGTT472000.11335944683471964No Hit
AGATAAGGGAAGTCGGCAAAATAGATCCGT470850.1130832532672198No Hit
GGAATGAGTACAATGTAAATACCTT465520.1118031561239379No Hit
AGCCGCGGTAATTCCAGCTCCAGTAGCGT464440.1115437743388076No Hit
AGTAAGCGCAAGTCATCAGCTTGCGTTGATT463220.11125076898893821No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT462300.11102981413493834No Hit
AGGTCTCCAAGGTGAACAGCCTCTAGTTGAT461540.11084728621206888No Hit
AACGATCAACTTAGAACTGGTACGGAC460700.1106455448236342No Hit
ATTGCGCCCTCTGGTATTCCAGGGGGCATGC459760.11041978660324302No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAG459040.11024686541315615No Hit
AGTGACGAAGCCTAGGCTGTAAAGCTGGGT458730.11017241323409098No Hit
AGCCAAGGGAACGGGCTTGGCAGAATC455140.10931020896685234No Hit
AAGCATATCAATAAGCGGAGGAAAAGAAACC450250.10813578588417906No Hit
ATTGAAGACTAACTACTGCGAAAGCATTTGCC446380.1072063344874622No Hit
ATAGGGGCGAAAGACTAATCGAACCATCT445060.10688931230563628No Hit
ATCATACCGAAGCAGAATTCGGTAAGC444230.10668997260039727No Hit
AGGCGTGGAGGTCAGTGACGAAGCCT443930.10661792210452774No Hit
AAAATCAGTTATCGTTTATTTGATAGTT443400.10649063289515824No Hit
ATCCGCTGGATTATGGCTGAACGCCTCT443080.10641377903289741No Hit
TTGACCTATTCTCAAACTTTAAATATGT442500.10627448140754966No Hit
TGACCTATTCTCAAACTTTAAATATGT435840.1046749603992462No Hit
AAATGCCTCGTCATCTAATTAGTGACGCGC433690.10415859851218127No Hit
AAGCGTGTTACCTATACTCCACCGTC429540.10316189998598618No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA427740.10272959701076903No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT426800.10250383879037786No Hit
TTAGGCCACCATCGAAAGGGAATCC425190.10211716779587807No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGAGGT418560.10052485183716156No Hit
TTTTTCATGCCAGGTCGTACTGATAACCGC417480.10026547005203128No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)