FastQCFastQC Report
Sat 24 Jun 2023
ERR6133650_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133650_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17635445
Total Bases522.2 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGCGAAGTCCCGCAACGCGA266231115.0963641688656No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT188416610.683971966684142No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT15795888.956893347460186No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA11048716.265058806284729No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT11040446.260369386766254No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT4175372.3676011577819556No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT3238911.8365910244964048No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA2173991.232738952717099No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA1872971.0620486185633535No Hit
ATGTACACGGCGAAGTCCCGCAACGCG1652090.9368008575910617No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC1457600.8265172781293583No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC1257210.7128881635819226No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT1122460.63647954446287No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC1075070.6096075262064553No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT1049410.5950572837827456No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT974920.5528184857257642No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGA864030.4899394373093505No Hit
AATACAGCCAGCTGGGACTGAGGATTGCGACT659130.37375297306078753No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT584220.3312760182688897No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAA550010.3118775851700935No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGA441750.25048985154613335No Hit
TTGGGCCAGCATCAGTTTTAGCGGTTGGATA438150.24844850810399172No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG431810.24485347548644223No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC411480.2333255554367922No Hit
TTGATGATTCATAATAACTTTTCGAATCGC377060.21380804397053774No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATT374730.21248684113159608No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCAT372430.21118264948800555No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATT365740.2073891529246923No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTC338360.19186360196751484No Hit
TACACGGCGAAGTCCCGCAACGCGA338240.19179555718611013No Hit
ATGACCCACTCGGCACCTTACGAGAAATC327190.18552976689842532No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAT320970.18200277906228055No Hit
TGTACACGGCGAAGTCCCGCAACGCGA296010.16784946453009833No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT243530.1380912134624332No Hit
TATGTACACGGCGAAGTCCCGCAACGCGA228120.12935312945037677No Hit
GTACACGGCGAAGTCCCGCAACGCGA213680.12116507408800854No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCT201500.11425852877542926No Hit
ATGCCGACTAGGGATCGGGTGGTGT194430.11024955707100105No Hit
GTACAACAGGGTATTGTAAGCAGTAGAGTAGC190920.10825924721491294No Hit
AAGCTCGAATATATTAGCATGGAATAATGGAA184650.10470390738651619No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTT180050.10209552409933519No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTC178320.1011145451674171No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)