FastQCFastQC Report
Sat 24 Jun 2023
ERR6133651_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133651_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5301901
Total Bases151.3 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTACACGGCGAAGTCCCGCAACGCGA179397433.83642961269929No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT1906223.595351931316711No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC1607253.0314598480809054No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGA1391342.6242285550031963No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT1076792.030950785388109No Hit
ACACGGAGTCGAGCTCAACCCGCAACGCGA482310.9096925800764668No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC476380.898507912539295No Hit
ATGTACACGGCGAAGTCCCGCAACGCG442940.8354361954325439No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT402670.7594823064406522No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT341520.6441463165758847No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA278800.5258491246818829No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC269480.5082705241006952No Hit
ATGTACACGGAGTCGAGCTCAACCCGC199610.3764876032200526No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT175590.3311830982887082No Hit
TGTACACGGCGAAGTCCCGCAACGCGA174180.3285236748102237No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT163420.30822906727228594No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA154880.2921216371260044No Hit
TACACGGCGAAGTCCCGCAACGCGA151840.2863878446617544No Hit
TATGTACACGGCGAAGTCCCGCAACGCGA148160.27944693799450426No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACGC144720.2729586991533791No Hit
ATGTACACGGCGAAGTCCCGCAACGC134460.2536071495865351No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTC134070.252871564369082No Hit
GTACACGGCGAAGTCCCGCAACGCGA119940.22622074610597218No Hit
TACACGGAGTCGAGCTCAACCCGCAACGCGA112150.2115279029163313No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAAC99860.1883475379868466No Hit
ATGTACACGGGCGAAGTCCCGCAACGCGA99410.18749878581286222No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT96870.18270805131970588No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT93180.17574828349303392No Hit
ATGTACACGGAGTCGAGCTCAACCCGCAACG78490.14804124030229912No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG76700.14466509276578346No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGT70450.13287686812711139No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT68870.1298968049384551No Hit
ATTCCAGGGGGCATGCCTGTTTGAGC68670.1295195817500176No Hit
ATGTACACGGCGAAGTCCCGCAACG64130.12095661537248621No Hit
CACGGAGTCGAGCTCAACCCGCAACGCGA61980.11690146609678304No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTT61860.11667513218372053No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA59240.1117335084151892No Hit
ATGTACACGGCGAAGTCCCGCAACACGA54890.10352890406667345No Hit
GTACACGGAGTCGAGCTCAACCCGCAACGCGA54770.10330257015361095No Hit
AATGTACACGGCGAAGTCCCGCAACGCGA54000.10185026087812656No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC53860.1015862046462203No Hit
ATGTACACGGAGTCGAGCTCAACCCGCA53270.10047339624032964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)