FastQCFastQC Report
Sat 24 Jun 2023
ERR6133652_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133652_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19720309
Total Bases584 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT209071010.601811563906022No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT18294629.277045303904721No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT8599354.36065682337939No Hit
ATGTACACGGCGAAGTCCCGCAACGCGA7881753.9967680019618355No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC2248381.1401342646304375No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT1914190.9706693743997623No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT1904930.9659737076127964No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1534370.7780659015028618No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA1506290.7638267737082619No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC1185450.6011315542773695No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT948410.48093059799417953No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT922330.4677056530909328No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC770240.390582115117973No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA630220.3195791708943303No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCC593900.3011616095873549No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT581630.29493959754890253No Hit
ATACGCGAAACTCAGGTGCTGCAATCT487480.2471969379384471No Hit
AATGAAAGTGAACGTAGGTGAGGGCCCG442510.22439303562636873No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT419760.21285670523722525No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTT410980.20840444234418437No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT402810.20426150523300624No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC363410.18428210227334674No Hit
AATACAGCCAGCTGGGACTGAGGATTGCGACT340660.17274577188420323No Hit
ATGACCCACTCGGCACCTTACGAGAAATC337520.17115350474477858No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC337470.17112815017249478No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGATCG335530.1701443927678821No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTGT331910.16830872173453265No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG328790.16672659642402154No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGA325880.16525096031710254No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT321850.16320738179102567No Hit
ATGTACACGGCGAAGTCCCGCAACGCG315690.16008369848565762No Hit
TTTGATGATTCATAATAACTTTTCGAATCGCA311850.15813646733425932No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATTT304140.15422679228809244No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCAT289370.1467370516354485No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCA288640.14636687488010458No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCC268780.13629603876896657No Hit
ATGCCGACTAGGGATCGGGTGGTGT264780.13426767298626No Hit
TAGGCCACCATCGAAAGGGAATCCGGTT260920.13231030000594818No Hit
TATCTGGTTGATCCTGCCAGTAGTC260740.1322190235457264No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATT254920.12926775133188836No Hit
ATGCGAGTGTTTGGGTGTAAAACCCGT245330.1244047443678494No Hit
AAGCTCGAATATATTAGCATGGAATAATGGAA242350.12289361185973302No Hit
ACGCGAAACTCAGGTGCTGCAATCT234430.11887744760997407No Hit
TTAGGCCACCATCGAAAGGGAATCCGGTT234290.11880645480757933No Hit
TATGACCCACTCGGCACCTTACGAGAAATC225330.11426291545431667No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCCT219110.11110880666220799No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTC209770.1063725725595882No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)