FastQCFastQC Report
Sat 24 Jun 2023
ERR6133653_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133653_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15920966
Total Bases463.1 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT10170506.388117404433877No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT6946444.363076964048538No Hit
ATGTACACGGCGAAGTCCCGCAACGCGA5212253.2738277313072586No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT3641942.287511951222055No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC2402811.5092111873111218No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC2058301.2928235635953247No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG1340490.8419652425612868No Hit
ATACGCGAAACTCAGGTGCTGCAATCT1206650.757899991746732No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1180360.7413871746224444No Hit
ATGACCCACTCGGCACCTTACGAGAAATC924150.580461009715114No Hit
AATGAAAGTGAACGTAGGTGAGGGCCCG909240.5710960000793921No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC833580.5235737580244817No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT819970.5150252817574008No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA765290.4806806320671748No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT735100.46171821483696407No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC715410.44935087481500813No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT643630.4042656708141956No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC635430.39911522956584417No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTGT548180.3443132784782029No Hit
TAGGCCACCATCGAAAGGGAATCCGGTT529520.3325928841252472No Hit
TATGACCCACTCGGCACCTTACGAGAAATC482200.3028710695067121No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA461060.28959298072742573No Hit
ATGCCGACTAGGGATCGGGTGGTGT458850.28820487400073586No Hit
ACGCGAAACTCAGGTGCTGCAATCT455690.2862200698123468No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGATCG448610.28177310346620926No Hit
ATGCGAGTGTTTGGGTGTAAAACCCGT427100.2682626167281558No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT421200.2645568114397079No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC399300.25080136469106207No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCC371380.2332647403430169No Hit
AGGGGATCGAAGATGATCAGATACCGTCGT356960.22420750097701359No Hit
ATAGCTGTTGGGACCCGAAAGATGGTGAACT345100.21675820424464196No Hit
AGCGAATTGCGATATGTATTGTGAATTGCAGA344680.21649440115631174No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCT341840.21471058979712662No Hit
ATCGATGAAGAACGCAGCGAATTGCGATATGT340550.21390033745439818No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCC335130.21049602140975615No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT329480.2069472417691238No Hit
AGGGTATTGTAAGCAGTAGAGTAGCC325650.20454160884458894No Hit
AAGAGATGGGGAAGCTCCGTTTCAAAGGCCTA321900.20218622412735507No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT314220.19736239622646012No Hit
ATTGCGATGGTCAGAAAGTGATGTTGACGC312760.19644536644321708No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGC298020.18718713424800984No Hit
ATGATTAATAGGGACGGTCGGGGGCATC278800.17511500244394718No Hit
TAGGCCACCATCGAAAGGGAATCCGGTTAAT277420.17424822086800512No Hit
ATGCCAGGTCGTACTGATAACCGCAGC276500.17367036648404374No Hit
AAGAAGTCCTTGTTGCTTAATTGAACGTGGAC275870.17327466185154847No Hit
GCGAAAAATCTGGTCAAACTTGGTCATTT275560.17307995004825713No Hit
ACCGTTTCCCGTCCGATCAACTGTAGTT261220.16407295888955484No Hit
ATCGATGAAGAACGCAGCGAATTGCGAT256720.16124649722887419No Hit
TATGGCGTCGCTGAACCATAGCAGGCTGGC248210.15590134417723145No Hit
AAGTCAGAATCCATGCTAGAACGCGATGATTT247230.15528580363779435No Hit
ACGTGAAATTGTTGAAAGGGAAGGGC244420.15352083535634709No Hit
AGCGAAAAATCTGGTCAAACTTGGTCATTT240180.15085768036939468No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT237890.14941932543540384No Hit
TTGATGATTCATAATAACTTTTCGAATCGC234150.1470702217440826No Hit
AGTTTTAGCGGTTGGATAAATCCTCGGG229310.14403020520237275No Hit
ATTTGAATGAAGAGCTTTTAGTGGGCC222140.13952670962302163No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTG220890.13874158138394369No Hit
AGGGTATTGTAAGCAGTAGAGTAGCCT220210.13831447162188523No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGATCC219340.137768022367487No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCCT218050.13695777002475854No Hit
AGAATCCATGCTAGAACGCGATGATTTC217290.13648041205539915No Hit
AGGGAGGTAGTGACAATAAATAACG216370.13590255767143777No Hit
TGGCGATGGTTCATTCAAATTTCTGCCCT213650.13419411862320416No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAG213550.1341313083640779No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATC213230.13393031553487394No Hit
ATCAGTTTTATGAGGTAAAGCGAATGATTAGA213010.13379213296479622No Hit
ATGTACACGGCGAAGTCCCGCAACGCG211800.13303212882936877No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTT210050.13193294929465962No Hit
AGGTCGTACTGATAACCGCAGCAGGTCTCC207970.1306264959048339No Hit
ACGGAGTCGAGCTCAACCCGCAACGCGA206870.12993558305444533No Hit
TATCTGGTTGATCCTGCCAGTAGTC206190.1295084732923869No Hit
TGGCGATGGTTCATTCAAATTTCTGCCCTATC201710.12669457368353151No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT200640.12602250391088077No Hit
ATAGAGGGTGTAGCATAAGTGGGAGCTT198970.12497357258347264No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATC194570.12220992118191823No Hit
ATTGGCGAGAGACCGATAGCGAACAAGT193460.121512727305617No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCC190260.11950279901357744No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGAG186350.11704691788174158No Hit
AATGATTAATAGGGACGGTCGGGGGCATC185920.11677683376749878No Hit
ATTGGCGAGAGACCGATAGCGAACAAGTAC185260.11636228605726562No Hit
TAATGATTAATAGGGACGGTCGGGGGCATC183310.11513748600430401No Hit
AAATATTGGCGAGAGACCGATAGCGAAC182950.11491136907144955No Hit
AGCAGGTCTCCAAGGTGAACAGCCTCT182850.11484855881232331No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGC177010.11118043967935111No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCCT176040.11057118016582661No Hit
ATCAGTTTTAGCGGTTGGATAAATCCTCGGG175540.11025712887019544No Hit
AAATCAGGTAGGAGTACCCGCTGAACTTAAGC174000.1092898508796514No Hit
ACGCGCGCTACACTGACGGAGCCAGCGAGTAC172530.10836654007049572No Hit
ACTGCGAAAGCATTTGCCAAGGACGTTTTC171850.10793943030843732No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGA169700.10658900973722324No Hit
ATAGCAGGCTGGCAACGGTGCTCTT165720.10408916142399902No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATC163460.10266964956774607No Hit
AGGAGTACCCGCTGAACTTAAGCATATC163410.10263824443818297No Hit
AGCTCTAAGTGGGTGGTAAATTCCATCT161650.1015327838775612No Hit
CCAGGGGGCATGCCTGTTTGAGCGTCATT160970.1011056741155028No Hit
ATTGCGCCCTCTGGTATTCCAGGGGGCATGC160210.1006283161461434No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)