FastQCFastQC Report
Sat 24 Jun 2023
ERR6133654_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133654_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14101193
Total Bases406.7 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT4923913.4918393074968903No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC3239622.2974084533131345No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT1792801.2713817901790296No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG1788601.268403318783028No Hit
ATGTACACGGCGAAGTCCCGCAACGCGA1594681.13088303947049No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1545361.0959072753631556No Hit
ATACGCGAAACTCAGGTGCTGCAATCT1455751.0323594606498896No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC1115680.7911954683550535No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC965370.6846016503710005No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC931520.6605965892389389No Hit
ATGACCCACTCGGCACCTTACGAGAAATC886110.6283936401693105No Hit
TATGACCCACTCGGCACCTTACGAGAAATC725830.5147294984190345No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTGT718780.5097299214328886No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT686520.48685242447217053No Hit
TAGGCCACCATCGAAAGGGAATCCGGTT618640.43871465343393284No Hit
ACGCGAAACTCAGGTGCTGCAATCT582820.4133125473851752No Hit
AATGAAAGTGAACGTAGGTGAGGGCCCG561070.3978883205130233No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC502250.35617553777187505No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC481360.34136118837604734No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGC470750.333837002301862No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCT448590.31812201988867184No Hit
AAGAGATGGGGAAGCTCCGTTTCAAAGGCCTA434580.3081866903034375No Hit
ATGCCGACTAGGGATCGGGTGGTGT431870.30626486709316014No Hit
AGGGGATCGAAGATGATCAGATACCGTCGT428310.30374025800512056No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT422680.29974768801476587No Hit
ATGCGAGTGTTTGGGTGTAAAACCCGT394660.2798770288442971No Hit
AGAATCCATGCTAGAACGCGATGATTTC383680.27209045362332107No Hit
ATGCCAGGTCGTACTGATAACCGCAGC382040.27092743145916803No Hit
ATCGATGAAGAACGCAGCGAATTGCGATATGT353960.2510142226973278No Hit
AGCGAAAAATCTGGTCAAACTTGGTCATTT346860.2459791877183725No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGATCG328990.23330650108824127No Hit
ATCGATGAAGAACGCAGCGAATTGCGAT328540.23298737915295534No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT317850.22540646029027472No Hit
AGCGAATTGCGATATGTATTGTGAATTGCAGA317720.2253142695089699No Hit
ACCGTTTCCCGTCCGATCAACTGTAGTT316090.22415833894337875No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCCT313580.22237834770433962No Hit
ATAGCTGTTGGGACCCGAAAGATGGTGAACT304510.21594626780868822No Hit
TAGGCCACCATCGAAAGGGAATCCGGTTAAT302820.21474778765172564No Hit
GCGAAAAATCTGGTCAAACTTGGTCATTT292210.2072236015775403No Hit
AGGGTATTGTAAGCAGTAGAGTAGCCT292170.20719523518329266No Hit
TTGATGATTCATAATAACTTTTCGAATCGC280680.19904698843565932No Hit
AGTTTTAGCGGTTGGATAAATCCTCGGG277620.1968769592757152No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA276700.19622453220801955No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATC273050.1936360987329228No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCC271170.19230287820328393No Hit
TATGGCGTCGCTGAACCATAGCAGGCTGGC263250.18668633214225208No Hit
ATTGCGATGGTCAGAAAGTGATGTTGACGC259030.18369367754912652No Hit
AGGTCGTACTGATAACCGCAGCAGGTCTCC245260.17392854632937796No Hit
AGCAGGTCTCCAAGGTGAACAGCCTCT244400.17331866885305378No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGATCC244180.17316265368469178No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT243500.17268042498248198No Hit
AAGAAGTCCTTGTTGCTTAATTGAACGTGGAC236250.16753901602509802No Hit
ACGTGAAATTGTTGAAAGGGAAGGGC229750.1629294769598572No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCCT229510.16275927859437142No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCC222350.15768169402404464No Hit
AGGGTATTGTAAGCAGTAGAGTAGCC221100.156795244203806No Hit
ACAGCCAAGGGAACGGGCTTGGCAGAATC217030.15390896358910908No Hit
ATGATGCCCTGACGAGTAGGCAGGCGT216900.1538167728078043No Hit
ATTGCGCCCTCTGGTATTCCAGGGGGCATGC215190.15260410945371786No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGAG210750.1494554396922303No Hit
AAGTCAGAATCCATGCTAGAACGCGATGATTT210590.14934197411523975No Hit
ATCAGTTTTATGAGGTAAAGCGAATGATTAGA209800.14878173782884896No Hit
ATTTGAATGAAGAGCTTTTAGTGGGCC209780.14876755463172514No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAG207510.1471577617581718No Hit
ACTGCGAAAGCATTTGCCAAGGACGTTTTC206510.14644860190198092No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT205300.14559051847598994No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA205260.1455621520817423No Hit
ATCAGACACCACAAAAGGTGTTAGTTCATCT205090.14544159490618985No Hit
TATCTGGTTGATCCTGCCAGTAGTCAT204780.14522175535077067No Hit
AGCTCTAAGTGGGTGGTAAATTCCATCT204720.14517920575939922No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTG204630.14511538137234203No Hit
CTTGATGATTCATAATAACTTTTCGAATCGC204530.14504446538672297No Hit
ATCGGTTTCAAGCCGATGGAAGTTTGAGGC204250.1448459006269895No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT203260.14414383236936054No Hit
ATCAGTTTTAGCGGTTGGATAAATCCTCGGG200410.1421227267792165No Hit
AAATCAGGTAGGAGTACCCGCTGAACTTAAGC198940.1410802617906159No Hit
AAATATTGGCGAGAGACCGATAGCGAAC197870.14032146074449162No Hit
TAATGATTAATAGGGACGGTCGGGGGCATC197640.1401583539775677No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT193370.1371302413916326No Hit
AGGTAGGAGTACCCGCTGAACTTAAGCATATC190520.1351091358014886No Hit
TACGGGCTGATCCGGGTTGAAGACATTGTC188900.1339602968344593No Hit
AGGAGTACCCGCTGAACTTAAGCATATC188380.13359153370924007No Hit
AAATCAGGTAGGAGTACCCGCTGAACTT183950.13044995554631442No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC182130.129159284608047No Hit
ATAGCAGGCTGGCAACGGTGCTCTT180030.12767004891004613No Hit
AGGTAGGAGTACCCGCTGAACTTAAGC178530.1266063091257598No Hit
ATGATTAATAGGGACGGTCGGGGGCATC178340.12647156875308352No Hit
AAGGTGAACAGCCTCTAGTTGATAGAAT173700.12318106702035778No Hit
ATTGGCGAGAGACCGATAGCGAACAAGT173650.12314560902754823No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCTAAAT173480.12302505185199579No Hit
ATATCAATAAGCGGAGGAAAAGAAACC173340.12292576947212906No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGATCCC169630.12029478640566085No Hit
ATTGGCGAGAGACCGATAGCGAACAAGTAC166010.11772762772624983No Hit
TATCTGGTTGATCCTGCCAGTAGTC165670.11748651337514493No Hit
TTTTCATGCCAGGTCGTACTGATAACCGC165210.11716029984129711No Hit
AAGTCTGGTGCCAGCAGCCGCGGTAATTCC160650.11392653089706666No Hit
AAATCAGTTATCGTTTATTTGATAGTT160060.11350812658191402No Hit
AAAACGGCGGGAGTAACTATGACTCTCTT157020.11135228061909372No Hit
TTGCTGGTTAACTCTTCTTAGAGGGACT153810.10907587748072095No Hit
AGACACCACAAAAGGTGTTAGTTCATCTAGAC153780.10905460268503522No Hit
AGTTTTATGAGGTAAAGCGAATGATTAGA152670.10826743524466334No Hit
AGCGAATTGCGATATGTATTGTGAATTGCAG151810.10765755776833917No Hit
AACGGCGAGTGAAGCGGCAAAAGCTC149780.10621796326027166No Hit
ATCTACCAGAAAGCACCGTTTCCCGTCCGATC149460.10599103210629057No Hit
AATGATTAATAGGGACGGTCGGGGGCATC149090.10572864295949995No Hit
AGCTGTTGGGACCCGAAAGATGGTGAACT148730.10547334541127122No Hit
AGAATCCATGCTAGAACGCGATGATTT148720.10546625381270933No Hit
ACGCGCGCTACACTGACGGAGCCAGCGAGTAC148460.10528187225009968No Hit
TTCGACGAGTCGAGTTGTTTGGGAATGC147870.10486346793494707No Hit
AGTAAGCGCAAGTCATCAGCTTGCGTTGATT146870.10415430807875618No Hit
TGGCGATGGTTCATTCAAATTTCTGCCCTATC146170.10365789617942255No Hit
TAATGATTAATAGGGACGGTCGGGGGC145220.10298419431604121No Hit
AAGGACGTTTTCATTAATCAAGAACGAAAGTT144680.10260124799369812No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT144310.1023388588469075No Hit
AGGGAGGTAGTGACAATAAATAACG141650.10045249362943971No Hit
AGTGTAGTGGGTGACCATACGCGAAACTC141150.10009791370134428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)