FastQCFastQC Report
Sat 24 Jun 2023
ERR6133655_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133655_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16337764
Total Bases473.3 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT8748975.355059603015444No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT4399742.692987853172564No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT2146371.3137477074586215No Hit
ATGTACACGGCGAAGTCCCGCAACGCGA1966331.203549029108267No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1568020.9597518975056807No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA1251770.7661819573351653No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA1053280.6446904239772346No Hit
ATACGCGAAACTCAGGTGCTGCAATCT904490.553619210070607No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC779250.4769624533687719No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC763960.4676037675657452No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT725400.4440020066393418No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG584090.35750914262196465No Hit
TAGGCCACCATCGAAAGGGAATCCGGTT505070.30914267093097925No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC471350.2885033716976203No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT444900.2723138857924499No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT413310.2529783145355754No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTGT412280.25234787330751013No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT407150.24920790874442797No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC368840.22575916753357436No Hit
ATGCGAGTGTTTGGGTGTAAAACCCGT365180.22351895889792506No Hit
AATGAAAGTGAACGTAGGTGAGGGCCCG363390.22242333773458842No Hit
ACGCGAAACTCAGGTGCTGCAATCT360500.22065442982283257No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCC349700.21404397811108056No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGATCC338900.20743352639932855No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT328490.20106178544383432No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC303950.1860413701654645No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT294120.1800246349500458No Hit
ATGCCGACTAGGGATCGGGTGGTGT284660.17423436891364083No Hit
GCGAAAAATCTGGTCAAACTTGGTCATTT271240.1660202705829268No Hit
ATCAGTTTTAGCGGTTGGATAAATCCTCGGG258400.1581611779922883No Hit
TATGACCCACTCGGCACCTTACGAGAAATC255490.15638002850328847No Hit
AGCGAAAAATCTGGTCAAACTTGGTCATTT255480.15637390771466647No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC246630.15095700978420304No Hit
ATGACCCACTCGGCACCTTACGAGAAATC243770.14920646423831316No Hit
AGTTTTAGCGGTTGGATAAATCCTCGGG242590.14848421118091804No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATC235470.1441262096820593No Hit
TAGGCCACCATCGAAAGGGAATCCGGTTAAT232250.1421553157457777No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCC211730.12959545749344892No Hit
AGAATCCATGCTAGAACGCGATGATTTC204100.12492529577486859No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT196760.12043263692632603No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCT195330.11955736415338108No Hit
ATCGATGAAGAACGCAGCGAATTGCGAT191930.1174762960219036No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCCT191260.1170662031842301No Hit
AAATCAGTTATCGTTTATTTGATAGTT190280.11646636589927484No Hit
TATGGCGTCGCTGAACCATAGCAGGCTGGC186320.11404253360496577No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGATCCC183030.11202879414833021No Hit
CAGGGGGCATGCCTGTTTGAGCGTCATTT180440.11044350989523413No Hit
CTTTTACTTTGAAAAAATTAGAGTGTTC175210.10724233744593202No Hit
ATTGCGCCCTCTGGTATTCCAGGGGGCATGC172900.10582843527425173No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACTT172830.10578558975389778No Hit
TTGCAGTTAAAAAGCTCGTAGTTGAACTTTGG167360.10243751837766785No Hit
TTGATGATTCATAATAACTTTTCGAATCGC167280.1023885520686919No Hit
ATAGAGGGTGAGAATCCCGTGTGGCGAGGATC166820.10210699579208024No Hit
AAGTCAGAATCCATGCTAGAACGCGATGATTT166400.10184992266995656No Hit
TCGACGAGTCGAGTTGTTTGGGAATGCAGCTC165380.1012256022305133No Hit
AGGGGATCGAAGATGATCAGATACCGTCGT163560.10011161870131066No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)