FastQCFastQC Report
Sat 24 Jun 2023
ERR6133656_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133656_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16450824
Total Bases472.9 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT7916074.81195957114367No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT4446982.7031959007038187No Hit
ATGTACACGGCGAAGTCCCGCAACGCGA2368301.4396239361627114No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT1963201.1933748728939049No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC1609600.9784312323808217No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC1569980.954347332388943No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1421970.8643761552612806No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC1318730.8016194204010693No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG1029650.6258956998141856No Hit
ATACGCGAAACTCAGGTGCTGCAATCT1004330.610504373519527No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT750780.4563783552726599No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA733040.44559470090981457No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC669870.40719540857041564No Hit
AATGAAAGTGAACGTAGGTGAGGGCCCG605020.367774890789665No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC549540.3340501363335964No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA547620.3328830215434801No Hit
ACGCGAAACTCAGGTGCTGCAATCT482610.2933652441968864No Hit
TAGGCCACCATCGAAAGGGAATCCGGTT429490.26107506833700245No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT427590.2599201109926166No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT407950.24798149928538532No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT355970.2163842978321329No Hit
ATGACCCACTCGGCACCTTACGAGAAATC354040.21521110431915141No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTGT338940.20603223279271604No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT294890.17925545857155847No Hit
TATCTGGTTGATCCTGCCAGTAGTCAT290640.17667200135385314No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGATCC281110.17087897846332803No Hit
TAGGCCACCATCGAAAGGGAATCCGGTTAAT278220.1691222275552884No Hit
TATCTGGTTGATCCTGCCAGTAGTC273420.1662044405799977No Hit
AAGGGTGCATCATCGACCGATCCTGA271040.1647577045380827No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCC263590.1602290560035169No Hit
AGCGAATTGCGATATGTATTGTGAATTGCAG262950.1598400177401448No Hit
ATGCGAGTGTTTGGGTGTAAAACCCGT261390.15889173697317532No Hit
AAATCAGTTATCGTTTATTTGATAGTT251790.1530561630225939No Hit
AGCGAATTGCGATATGTATTGTGAATTGCAGA250940.15253947157905282No Hit
AAGAGATGGGGAAGCTCCGTTTCAAAGGCCTA250880.1525029992418617No Hit
GCGAAAAATCTGGTCAAACTTGGTCATTT243450.14798650815302625No Hit
ATGCCAGGTCGTACTGATAACCGCAGC232810.14151874702446515No Hit
AGGGTATTGTAAGCAGTAGAGTAGCC230800.14029692372856217No Hit
AGCGAAAAATCTGGTCAAACTTGGTCATTT229230.13934256423872748No Hit
AGAATCCATGCTAGAACGCGATGATTTC226230.13751894737917078No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCC225980.13736697930754108No Hit
AGTTTTAGCGGTTGGATAAATCCTCGGG224750.13661929639512282No Hit
TATGACCCACTCGGCACCTTACGAGAAATC220650.13412702002039534No Hit
ATGCCGACTAGGGATCGGGTGGTGT217880.13244321378673798No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATC213640.12986583529189785No Hit
AGGGTATTGTAAGCAGTAGAGTAGCCT213200.12959837148582953No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC206500.1255256271661529No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAG200970.12216409342170338No Hit
ATGATGCCCTGACGAGTAGGCAGGCGT200780.12204859768726478No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT197620.12012772126186505No Hit
ATCGATGAAGAACGCAGCGAATTGCGAT192040.1167357939030896No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGATCG190760.11595771737634541No Hit
TATGGCGTCGCTGAACCATAGCAGGCTGGC187930.1142374388054969No Hit
ATCGATGAAGAACGCAGCGAATTGCGATATGT184830.11235303471728833No Hit
TTGATGATTCATAATAACTTTTCGAATCGC183780.11171476881644347No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTG182320.11082727527812587No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT177570.1079398819171611No Hit
AAACTATGCCGACTAGGGATCGGGTGGTGT172230.10469384390715018No Hit
ATCAGTTTTAGCGGTTGGATAAATCCTCGGG172050.10458442689557677No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCCT171340.10415283757214835No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGC170910.10389145248894524No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)