FastQCFastQC Report
Mon 26 Jun 2023
ERR6133657_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR6133657_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27960527
Total Bases799.8 Mbp
Sequences flagged as poor quality0
Sequence length25-32
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTCCAGGGGGCATGCCTGTTTGAGCGTCATT11912664.260527707507087No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATTT8581373.069101666073747No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTCATT7153672.5584889726863875No Hit
TCGACGAGTCGAGTTGTTTGGGAATGC5229881.87045115422896No Hit
ATGTACACGGCGAAGTCCCGCAACGCGA2383590.8524839320803932No Hit
ATACGCGAAACTCAGGTGCTGCAATCT2001950.7159915118910313No Hit
ATTAAATCAGTTATCGTTTATTTGATAGTT1907060.682054383309728No Hit
TTGATGATTCATAATAACTTTTCGAATCGCA1650760.5903894443763524No Hit
AAGGGTGCATCATCGACCGATCCTGA1494630.5345500104486586No Hit
ACGCGAAACTCAGGTGCTGCAATCT1453260.5197541519871925No Hit
TATCTGGTTGATCCTGCCAGTAGTCAT1338330.4786497765224525No Hit
ATTCCAGGGGGCATGCCTGTTTGAGCGTC1228260.439283565721061No Hit
TTCCAGGGGGCATGCCTGTTTGAGCGTC1182460.4229033308277773No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATTT1118730.40011048432670815No Hit
AAACTTTCAACAACGGATCTCTTGGTTCTCGC1073520.3839412611929668No Hit
AGTTAAAAAGCTCGTAGTTGAACTTTGG1047640.3746853555371113No Hit
TCCAGGGGGCATGCCTGTTTGAGCGTCATT1032220.36917043802500576No Hit
ATGACCCACTCGGCACCTTACGAGAAATC1006320.3599073794281488No Hit
CTTGATGATTCATAATAACTTTTCGAATCGCA900310.32199321565004835No Hit
AATGAAAGTGAACGTAGGTGAGGGCCCG699540.25018841740715403No Hit
TAGGCCACCATCGAAAGGGAATCCGGTT677290.2422307705430588No Hit
AGGGTATTGTAAGCAGTAGAGTAGCC646710.23129392375186636No Hit
TATCTGGTTGATCCTGCCAGTAGTC646490.23121524140085054No Hit
GTTTTCATGCCAGGTCGTACTGATAACCGC639570.22874032381435444No Hit
AACTGGAGGGCAAGTCTGGTGCCAGC609380.21794295937268993No Hit
TTGATGATTCATAATAACTTTTCGAATCGCAT589860.21096168895529044No Hit
ATGCGAGTGTTTGGGTGTAAAACCCGT588460.21046098308519007No Hit
AAGGGTGCATCATCGACCGATCCTG545970.19526455992764372No Hit
ATGCCAGGTCGTACTGATAACCGCAGC519610.1858369836877538No Hit
ATTTGAATGAAGAGCTTTTAGTGGGCC513750.18374117197433368No Hit
TATGACCCACTCGGCACCTTACGAGAAATC479250.17140234874686017No Hit
AGGGGGCATGCCTGTTTGAGCGTCATT431020.1541530315219023No Hit
ATGCCGACTAGGGATCGGGTGGTGT430290.15389194917534996No Hit
ACCGTTTCCCGTCCGATCAACTGTAGTT425640.15222889039251658No Hit
GCGAAAAATCTGGTCAAACTTGGTCATTT418980.14984696103903908No Hit
GTGAATCATCAAATCTTTGAACGCAC417510.14932121987543368No Hit
ATATGCTTGTCTCAAAGATTAAGCCATGC411040.1470072434614698No Hit
ATCGATGAAGAACGCAGCGAATTGCGAT401070.143441502372255No Hit
ACAGCCAGCTGGGACTGAGGATTGCGACT400600.1432734082587213No Hit
AGTTTTAGCGGTTGGATAAATCCTCGGG397950.1423256435760313No Hit
ACCATACGCGAAACTCAGGTGCTGCAATCT396590.14183924358793382No Hit
AGGGGGCATGCCTGTTTGAGCGTCATTT389220.1392033848289054No Hit
ACAACAGGGTATTGTAAGCAGTAGAGTAGCC383180.13704319664647235No Hit
AGCAGGTCTCCAAGGTGAACAGCCTCT379310.1356591025626949No Hit
TTTCCAGGGGGCATGCCTGTTTGAGCGTCATT379100.13558399668217982No Hit
ATAAACTATGCCGACTAGGGATCGGGTGGTGT378140.13524065551411102No Hit
ACGTGAAATTGTTGAAAGGGAAGGGC373430.13355613790827334No Hit
AAATCAGTTATCGTTTATTTGATAGTT370090.13236159676103387No Hit
AGCGAATTGCGATATGTATTGTGAATTGCAGA366380.13103472620526788No Hit
AGGGTATTGTAAGCAGTAGAGTAGCCT364860.1304911026891589No Hit
AGGGAGGTAGTGACAATAAATAACG363120.12986879682203414No Hit
ATTGCGATGGTCAGAAAGTGATGTTGACGC361100.12914634978088932No Hit
ATGATGCCCTGACGAGTAGGCAGGCGT354800.12689317336543765No Hit
AACAGGGTATTGTAAGCAGTAGAGTAGCC352700.1261421145602871No Hit
ACGATCAACTTAGAACTGGTACGGAC351630.12575943221671038No Hit
AGAGGGTGAGAATCCCGTGTGGCGAGGATCC351240.12561994986718242No Hit
AGAATCCATGCTAGAACGCGATGATTT350840.12547689104715373No Hit
AGAATCCATGCTAGAACGCGATGATTTC349790.12510136164457844No Hit
ATTGCGCCCTCTGGTATTCCAGGGGGCATGC330290.11812724416818036No Hit
ATCGATGAAGAACGCAGCGAATTGCGATATGT327410.11709722066397389No Hit
AAGTGGGTGGTAAATTCCATCTAAAGCT326620.11681467949441725No Hit
TATGGCGTCGCTGAACCATAGCAGGCTGGC313150.11199717372995151No Hit
ATCAGTTTTAGCGGTTGGATAAATCCTCGGG312580.11179331491141065No Hit
ATAGCTGTTGGGACCCGAAAGATGGTGAACT311190.111296185511811No Hit
AAGAAGTCCTTGTTGCTTAATTGAACGTGGAC302610.10822757382219583No Hit
ATGCTTGTCTCAAAGATTAAGCCATGC299380.10707237385046427No Hit
TAGGCCACCATCGAAAGGGAATCCGGTTAAT293540.10498371507804558No Hit
AAAGATTAAGCCATGCATGTCTAAGTATAAG293300.10489787978602835No Hit
TTGATGATTCATAATAACTTTTCGAATCGC290910.104043103336357No Hit
TTGCTGGTTAACTCTTCTTAGAGGGACT287900.1029665857156412No Hit
ATACAGTGAAACTGCGAATGGCTCATTAAATC287610.1028628680711204No Hit
AACTTCGGGATAAGGATTGGCTCTAAGGATCG283530.10140366810682788No Hit
AGCGAAAAATCTGGTCAAACTTGGTCATTT282480.1010281387042526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)