FastQCFastQC Report
Tue 27 Jun 2023
ERR618770_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR618770_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194819312
Total Bases4.8 Gbp
Sequences flagged as poor quality0
Sequence length25
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGCTGAGGCGTGATGACGAGGCA45847172.35331751915847No Hit
GAGGCTGAGGCGTGATGACGAGGCA30012191.5405141149456478No Hit
GCGGGAATAGCTCAGTTGGTAGAGC24012471.2325508058461883No Hit
CCTGCGGTTGGATCACCTCCTTAAA17702230.9086486251424603No Hit
AGCGAAGCTCTTGATCGAAGCCCCG16849430.8648747306940494No Hit
ACGGACGTGGGGGTTCAAGTCCCCC15411230.7910524804645651No Hit
GGTTAAGCGACTAAGCGTACACGGT14888590.7642255712308439No Hit
AAATGCCCTGCTTCCAGGAAAAGCC12689560.651350211112541No Hit
AAGTCATCATGGCCCTTACGACCAG10971340.5631546424925267No Hit
AGGTTAATGAGGCGAACCGGGGGAA10739650.5512620843256032No Hit
GCTCAGTTGGTAGAGCAGCGCAAAA9791770.5026077702194124No Hit
ACCGCCAGCTAAGGTCCCAAAGTCA9728350.4993524461271067No Hit
ATCAGGTAACATCAAATCGTACCCC9438150.48445659227048293No Hit
AGGCTGAGGCGTGATGACGAGGCAC8284810.4252560957611841No Hit
GGAGCGGTAGTTCAGTCGGTTAGAA8124140.41700896674966187No Hit
ATGAAAGCTACTAAACTGGTACTGG8032330.41229639492823994No Hit
CCTGCGGTTGGATCACCTCCTAAAA8011000.411201534270894No Hit
GCGAAGGTGGCGGAATTGGTAGACG7277440.3735481829439989No Hit
AGAATGCCACGGTGAATACGTTCCC7081490.3634901451658961No Hit
AGACCGCCAGCTAAGGTCCCAAAGT6979690.35826479050495774No Hit
CCTGCGGTTGGATCACCTCCAAAAA6270850.32188030722539457No Hit
TAGGCTGAGGCGTGATGACGAGGCA6232400.31990668358381225No Hit
AGTAGGTAGCTTAACCTTCGGGAGG5975210.306705220270976No Hit
GGGGCTGATTCTGGATTCGACGGGA5882680.3019556911277872No Hit
GGGGCTATAGCTCAGCTGGGAGAGC5871160.30136437397951593No Hit
CGGACGTGGGGGTTCAAGTCCCCCC5779350.29665180215809406No Hit
GAGTCATCATGGCCCTTACGACCAG5613450.2881362192676258No Hit
TCAAACCGGGAGATAGCTGGTTCTC5476460.2811045755053277No Hit
AATGCCCTGCTTCCAGGAAAAGCCT5412860.2778400120825804No Hit
AGGTAACATCAAATCGTACCCCAAA5282560.27115176343503355No Hit
ACACGGCGGGTGCTAACGTCCGTCG4976620.25544798146089337No Hit
GCATCCGTAGCTCAGCTGGATAGAG4838200.24834293635119706No Hit
AGGCTTGTAGCTCAGGTGGTTAGAG4609080.23658229529113622No Hit
AAGCGTGTAGGCTGGTTTTCCAGGC4427470.22726032417155853No Hit
ATGCGTTGTTGGGTAGGGGAGCGTT4344860.22301998479493657No Hit
TAGGTTAATGAGGCGAACCGGGGGA4228720.21705856347547312No Hit
GGTAGGTAGCTTAACCTTCGGGAGG4188930.21501615815171343No Hit
AATAGTCGCAAACGACGAAAACTAC3851820.19771243212274564No Hit
ATCTAAGTACCCCGAGGAAAAGAAA3744660.19221195073309774No Hit
GATGCCCTGCTTCCAGGAAAAGCCT3689380.18937444969521297No Hit
TTAGGTTAATGAGGCGAACCGGGGG3628520.18625052941363432No Hit
AAATTGAAGAGTTTGATCATGGCTC3617140.18566639841126223No Hit
TATAGTCGCAAACGACGAAAACTAC3446770.17692137214815748No Hit
AGGTAGCGCCTCGTGAATTCATCTC3431050.17611447062291236No Hit
CGGAGGTTCGAATCCTCCCGGATGC3393050.1741639453074344No Hit
AAGTAGGTAGCTTAACCTTCGGGAG3373900.17318098320766062No Hit
GCGAAGCTCTTGATCGAAGCCCCGG3348350.17186951158106953No Hit
AGGCGTGTGACTGCGTACCTTTTGT3290300.16888982751360912No Hit
TCCTCTGTAGTTCAGTCGGTAGAAC3267820.1677359378006632No Hit
GGCTGAGGCGTGATGACGAGGCACA3238600.16623608649228777No Hit
GGACCGCCAGCTAAGGTCCCAAAGT3031810.1556216357031381No Hit
GAATGCCCTGCTTCCAGGAAAAGCC2896820.14869265116797045No Hit
TGAAAGCTACTAAACTGGTACTGGG2878640.14775947879335494No Hit
AGGGCTTACGGGTTCAAGTCCCGTC2870100.14732112389350807No Hit
GGAATGCCACGGTGAATACGTTCCC2841420.14584899057645784No Hit
AAATAGTCGCAAACGACGAAAACTA2816040.14454624498417282No Hit
ATGCAAGTCGAACGGTAACAGGAAG2762470.14179651758548453No Hit
TACGGACGTGGGGGTTCAAGTCCCC2759640.141651254779095No Hit
AAGCAGTGGGAGCACGCTTAGGCGT2698780.13852733449751634No Hit
TCCCCATGCGAGAGTAGGGAACTGC2677820.13745146579718956No Hit
ATGCCCTGCTTCCAGGAAAAGCCTC2652350.13614410053968365No Hit
AAATGAATTGACGGGGGCCCGCACA2573710.1321075397289156No Hit
TGATCGAAGCCCCGGTAAACGGCGG2557820.1312919121693644No Hit
AGCAGTGGGAGCACGCTTAGGCGTG2549950.13088794811060622No Hit
AGCGAAATTCCTTGTCGGGTAAGTT2527820.1297520237624081No Hit
ACTTGCCCCGAGATGAGTTCTCCCT2508570.12876392870127784No Hit
GAGCGTGTAGGCTGGTTTTCCAGGC2493100.12796985957942406No Hit
AGGCACGCTGATATGTAGGTGAAGC2448560.1256836385912296No Hit
AAACGATGTCGACTTGGAGGTTGTG2344760.12035562470316084No Hit
TCGAAGCCCCGGTAAACGGCGGCCG2265230.11627338053631973No Hit
ATGCAAGTCGAACGGTAACAGGAAA2243090.11513694289198599No Hit
GCCGATATAGCTCAGTTGGTAGAGC2192870.11255916969874116No Hit
ATGGCCCTTACGACCAGGGCTACAC2179140.11185441410449083No Hit
GATAGTCGCAAACGACGAAAACTAC2132490.10945988763167382No Hit
ACGGTCCAGACTCCTACGGGAGGCA2091540.10735793995617848No Hit
GGGTCGTTAGCTCAGTTGGTAGAGC2075470.10653307306618556No Hit
ATAATGGGTCAGCGACTTATATTCT2030480.10422375375188676No Hit
ACATGCAAGTCGAACGGTAACAGGA2029810.10418936291079808No Hit
TCGCGCTGTGCGGGTTCAAGTCCCG2001510.10273673484690266No Hit
TTCGCCATTTAAAGTGGTACGCGAG2000710.10269567115605048No Hit
TAGGCGTGTGACTGCGTACCTTTTG1995120.10240873861622095No Hit
AGAAGTGCGAATGCTGACATAAGTA1993630.1023322574920088No Hit
GTGTAGGCTGGTTTTCCAGGCAAAT1972060.10122507772740723No Hit
TGAGGCGTGGCTTCCGGAGCTAACG1969310.1010839212901029No Hit
GCTGATATAGCTCAGTTGGTAGAGC1960130.10061271543757427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)